Gsn (NM_001206369) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ADF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231015 representing NM_001206369 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGTGGAGCACCCCGAATTCCTGAAGGCAGGGAAGGAGCCTGGCCTGCAGATCTGGCGTGTGGAGA AGTTTGACCTGGTGCCTGTGCCCCCCAACCTCTATGGAGACTTCTTCACGGGTGATGCCTATGTCATCCT AAAGACTGTGCAGCTGAGGAATGGGAATCTGCAGTATGACCTCCACTATTGGCTGGGCAATGAATGCAGC CAGGATGAGAGCGGGGCTGCTGCCATCTTTACTGTGCAACTGGATGACTACCTGAACGGCCGGGCTGTAC AGCACCGTGAGGTTCAGGGCTTTGAGTCGTCCACCTTCTCCGGCTACTTCAAGTCTGGACTTAAGTACAA GAAAGGAGGTGTGGCATCTGGATTCAAACACGTGGTACCCAATGAGGTGGTGGTCCAGAGGCTCTTCCAG GTCAAAGGACGCCGTGTAGTCCGTGCTACTGAGGTACCTGTGTCCTGGGACAGTTTCAACAATGGCGACT GCTTCATTCTGGACCTGGGAAACAATATCTATCAGTGGTGTGGCTCTGGCAGCAACAAATTTGAAAGGCT GAAGGCCACACAGGTGTCCAAGGGCATCCGGGACAACGAGAGGAGTGGCCGTGCTCAAGTACACGTGTCT GAAGAGGGAGGAGAGCCAGAAGCCATGCTGCAGGTGCTGGGCCCCAAGCCAGCTCTGCCTGAAGGTACCG AGGACACAGCCAAGGAAGATGCAGCCAACCGAAGGCTGGCCAAGCTCTACAAGGTCTCCAACGGTGCAGG TAGCATGTCAGTCTCCCTAGTGGCTGATGAGAACCCCTTCGCCCAGGGCGCCCTGAGATCTGAGGACTGC TTCATCCTGGACCATGGCAGAGATGGGAAAATCTTTGTTTGGAAAGGCAAGCAGGCCAACATGGAGGAGC GGAAGGCTGCCCTCAAAACAGCCTCTGACTTCATCTCCAAGATGCAGTACCCCAGGCAGACCCAGGTTTC AGTTCTCCCAGAGGGCGGTGAGACCCCTCTCTTTAAGCAGTTCTTCAAGAACTGGCGGGACCCAGACCAG ACAGACGGCCCCGGCCTGGGCTACCTCTCCAGCCACATTGCCAACGTGGAGCGCGTACCTTTCGATGCTG CTACGCTGCACACCTCCACCGCCATGGCCGCTCAGCACGGCATGGATGATGATGGAACTGGCCAGAAACA GATCTGGAGAATTGAAGGTTCCAACAAGGTGCCAGTGGACCCTGCCACATACGGGCAGTTCTATGGAGGC GACAGCTACATCATTCTGTACAACTACCGCCACGGTGGCCGCCAGGGACAGATCATCTACAACTGGCAGG GTGCCCAGTCTACCCAGGATGAGGTTGCTGCTTCTGCCATCCTGACTGCCCAGCTGGATGAGGAGCTGGG AGGAACTCCTGTCCAGAGCCGAGTGGTCCAAGGCAAAGAGCCTGCACACCTCATGAGCTTGTTTGGCGGG AAGCCCATGATCATCTACAAGGGTGGCACCTCCCGTGATGGTGGGCAGACAGCTCCTGCCAGTATCCGCC TCTTCCAAGTGCGAGCCAGCAGCTCTGGAGCCACCAGGGCTGTGGAGGTGATGCCTAAGTCTGGTGCTCT GAACTCCAACGATGCCTTTGTGCTGAAAACCCCCTCCGCTGCCTACCTGTGGGTGGGCGCAGGAGCCAGT GAGGCGGAGAAGACCGGCGCCCAGGAGCTTCTGAAGGTGCTTCGGTCCCAGCATGTGCAGGTGGAAGAAG GCAGTGAGCCAGATGCCTTCTGGGAGGCTCTGGGCGGGAAGACTGCCTACCGCACATCCCCCAGGCTTAA GGACAAGAAGATGGATGCCCATCCTCCTCGACTCTTTGCCTGCTCCAACAGGATCGGACGCTTTGTGATC GAAGAGGTTCCTGGCGAGCTTATGCAGGAAGACCTGGCTACTGATGACGTCATGCTCCTGGACACCTGGG ACCAGGTCTTTGTCTGGGTTGGAAAAGACTCCCAGGAAGAGGAAAAGACGGAAGCCTTGACTTCTGCTAA GCGGTACATCGAGACAGATCCAGCAAATCGGGACAGGCGGACCCCCATCACAGTCGTTAGGCAGGGCTTT GAGCCTCCTTCCTTCGTGGGCTGGTTCCTCGGCTGGGATGACAACTACTGGTCGGTGGATCCTTTGGACC GGGCCTTGGCTGAGCTGGCTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231015 representing NM_001206369 Red=Cloning site Green=Tags(s) MVVEHPEFLKAGKEPGLQIWRVEKFDLVPVPPNLYGDFFTGDAYVILKTVQLRNGNLQYDLHYWLGNECS QDESGAAAIFTVQLDDYLNGRAVQHREVQGFESSTFSGYFKSGLKYKKGGVASGFKHVVPNEVVVQRLFQ VKGRRVVRATEVPVSWDSFNNGDCFILDLGNNIYQWCGSGSNKFERLKATQVSKGIRDNERSGRAQVHVS EEGGEPEAMLQVLGPKPALPEGTEDTAKEDAANRRLAKLYKVSNGAGSMSVSLVADENPFAQGALRSEDC FILDHGRDGKIFVWKGKQANMEERKAALKTASDFISKMQYPRQTQVSVLPEGGETPLFKQFFKNWRDPDQ TDGPGLGYLSSHIANVERVPFDAATLHTSTAMAAQHGMDDDGTGQKQIWRIEGSNKVPVDPATYGQFYGG DSYIILYNYRHGGRQGQIIYNWQGAQSTQDEVAASAILTAQLDEELGGTPVQSRVVQGKEPAHLMSLFGG KPMIIYKGGTSRDGGQTAPASIRLFQVRASSSGATRAVEVMPKSGALNSNDAFVLKTPSAAYLWVGAGAS EAEKTGAQELLKVLRSQHVQVEEGSEPDAFWEALGGKTAYRTSPRLKDKKMDAHPPRLFACSNRIGRFVI EEVPGELMQEDLATDDVMLLDTWDQVFVWVGKDSQEEEKTEALTSAKRYIETDPANRDRRTPITVVRQGF EPPSFVGWFLGWDDNYWSVDPLDRALAELAA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001206369
ORF Size	2193 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001206369.1, NP_001193298.1
RefSeq Size	2601 bp
RefSeq ORF	2196 bp
Locus ID	227753
MW	81.2 kDa
Gene Summary	Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.[UniProtKB/Swiss-Prot Function]

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