Dyrk1b (NM_001271370) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Mirk
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230891 representing NM_001271370 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGCCGCTCGCCCACCGCACTGGGGTCCCCATCGCGCTCCAGCCCCCCGTGGGCCCAGCGCCATCC CTGACCCGGGTCTCAGCGGCGGTGGCAGCCGAGGTGCAGGATGCGAGAAGGCGCCCCCCGGCCGGGCTCC CGCTCCAGGCCTCACGCCCCTGCGGCCCTCTGAGCCCACCATGGCCGTCCCACCAGGCCATGGTCCTTTC TCTGGCTTTCCGGGGCCCCAGGAACACACACAGGTACTACCTGATGTGCGGCTCCTGCCCCGGAGACTGC CCCTGGCCTTCCGGGATGCGGCCTCAGCCCCGCTGCGCAAGCTCTCGGTGGACCTCATCAAGACCTACAA GCACATCAATGAGGTATACTATGCGAAGAAGAAGCGGCGGGCCCAACAGGCGCCACCCCAGGACTCGAGC ACCAAAAAGGAGAAGAAGGTCCTGAACCACGGTTATGATGACGACAACCACGACTACATTGTGCGCAGTG GCGAGCGCTGGCTAGAGCGCTATGAGATTGACTCTCTTATTGGCAAAGGCTCCTTTGGCCAGGTGGTGAA AGCCTATGATCACCAGACTCAGGAGCTGGTGGCCATCAAGATCATCAAGAACAAAAAGGCCTTCCTGAAC CAGGCACAGATTGAGCTACGGCTGTTGGAGCTGATGAACCAGCATGATACAGAGATGAAGTACTACATAG TACACCTTAAGCGGCACTTCATGTTCCGGAATCACCTGTGCCTGGTGTTTGAGCTGCTGTCCTACAACCT GTACGACCTCCTCCGCAACACACACTTTCGGGGTGTCTCACTGAACCTGACGAGGAAGCTGGCACAGCAG CTCTGCACAGCTCTGCTCTTTCTGGCCACCCCCGAGCTCAGCATCATCCACTGCGACCTCAAGCCTGAGA ACATCCTGCTCTGCAACCCCAAGCGCAGTGCCATCAAGATCGTGGACTTCGGCAGTTCCTGCCAGCTTGG CCAGCGGATCTACCAGTATATCCAGAGCCGCTTCTACCGCTCACCCGAGGTGCTCCTGGGTACACCCTAT GACCTGGCCATTGACATGTGGTCCCTGGGCTGCATCCTCGTGGAGATGCACACCGGAGAGCCCCTCTTCA GTGGCTCTAATGAGGTGGACCAGATGAGCCGTATTGTGGAGGTGTTGGGCATCCCTCCCGCACCCATGCT GGAACAGGCACCCAAGGCTCGAAAGTACTTTGAGCGGCTGCCTGGGGGTGGCTGGACCCTACGAAGGACA AAGGAACTCAGGAAGGATTACCAGGGCCCTGGGACACGGCGGCTGCAGGAGGTGCTGGGCGTGCAGACGG GCGGGCCCGGGGGCCGGCGGGCGGGGGAGCCCGGCCACAGCCCCGCCGACTACCTCCGCTTCCAGGACCT GGTGCTGCGCATGCTGGAATATGAGCCCGCCGCCCGCATCAGCCCTCTGGGCGCTCTGCAGCATGGCTTC TTCCGCCGCACGGCCGACGAGGCCACCAACACGGGCCCGGCAGGCAGCAGTGCCTCCACCTCGCCGGCGC CCCTTGACACCTGCCCCTCCTCTAGCACCGCCAGCTCCATCTCCAGCTCTGGAGGTTCCAGTGGCTCCTC CAACGACAACAGAGCCTACCGATACAGCAACCGATATTGTGGGGGCCCAGGGCCCCCCATCACTGACTGT GAGATGAACAGCCCCCAGGTCCTACCCTCCCAGCCTCTGCGCCCCTGGGCAGGGGGTGATGTGCCCCACA AGACACATCAAGCCCCTATCTCTGCCTCAACATTGCCGGGGACTGGGGCTCAGTTACCCCCATTGCCCCG TTGCCTTGGACGACCCCCATCACCAACATCACCACCACCCCCAGAGTTGATGGATGTGAGCCTGGTGGGC AGCCCTCCAGACTGCTCTCCACCTCCTCCAGCACCTGCCCCCCAGCACCCGGCTGCCTCAGCCCTCCGGA CTCGGATGACAGGAGGTCGACCACCTCTCCCACCCCCTGATGACCCTGCCACTCTGGGGCCTCGCCTGGG TCTCCATGGTGTACCCCAGAGCACAGCAGCCAGCTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230891 representing NM_001271370 Red=Cloning site Green=Tags(s) MLAARPPHWGPHRAPAPRGPSAIPDPGLSGGGSRGAGCEKAPPGRAPAPGLTPLRPSEPTMAVPPGHGPF SGFPGPQEHTQVLPDVRLLPRRLPLAFRDAASAPLRKLSVDLIKTYKHINEVYYAKKKRRAQQAPPQDSS TKKEKKVLNHGYDDDNHDYIVRSGERWLERYEIDSLIGKGSFGQVVKAYDHQTQELVAIKIIKNKKAFLN QAQIELRLLELMNQHDTEMKYYIVHLKRHFMFRNHLCLVFELLSYNLYDLLRNTHFRGVSLNLTRKLAQQ LCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGTPY DLAIDMWSLGCILVEMHTGEPLFSGSNEVDQMSRIVEVLGIPPAPMLEQAPKARKYFERLPGGGWTLRRT KELRKDYQGPGTRRLQEVLGVQTGGPGGRRAGEPGHSPADYLRFQDLVLRMLEYEPAARISPLGALQHGF FRRTADEATNTGPAGSSASTSPAPLDTCPSSSTASSISSSGGSSGSSNDNRAYRYSNRYCGGPGPPITDC EMNSPQVLPSQPLRPWAGGDVPHKTHQAPISASTLPGTGAQLPPLPRCLGRPPSPTSPPPPELMDVSLVG SPPDCSPPPPAPAPQHPAASALRTRMTGGRPPLPPPDDPATLGPRLGLHGVPQSTAASS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271370
ORF Size	2067 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271370.1, NP_001258299.1
RefSeq Size	2477 bp
RefSeq ORF	2070 bp
Locus ID	13549
UniProt ID	Q9Z188
MW	75.5 kDa
Gene Summary	Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activity. Enhances the transcriptional activity of TCF1/HNF1A and FOXO1. Inhibits epithelial cell migration. Mediates colon carcinoma cell survival in mitogen-poor environments. Inhibits the SHH and WNT1 pathways, thereby enhancing adipogenesis. In addition, promotes expression of the gluconeogenic enzyme glucose-6-phosphatase (G6PC).[UniProtKB/Swiss-Prot Function]

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