Zfp219 (NM_001253696) Mouse Tagged ORF Clone
CAT#: MR230871
- TrueORF®
Zfp219 (myc-DDK-tagged) - Mouse zinc finger protein 219 (Zfp219), transcript variant 4
"NM_001253696" in other vectors (1)
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CNY 6,840.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2010302A17Rik; Znf219 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR230871 representing NM_001253696
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGCAGTGGGTTGGTCTGAGACTCGTGCAGGCGAACGGCGCTTCCCTTGTCCTGTGTGCGGAAAGC GCTTCCGATTCAATTCCATCTTGGCTTTGCACCTGAGAGCCCACCCGGGCGCCCAAGCCTTCCAGTGCCC ACATTGCGGCCACCGCGCAGCGCAGCGGGCTCTGCTGCGCTCACACCTCCGAACGCACCAGCCGGAGCGT CCACGCAGCCCTGCTGCACGGCTGTTGCTGGAGTTGGAGGAACGCGCCCTGCTTCGCGAAGCCCGACTCG GGAGAGCCAGAAGCTCAGGCGGCATGCAGTCCAGCCCTGCCGCAGAGGGCCTGGCTCGCCCTCAGGTCCC TTCCTCATCTGCCTTCCGTTGCCCTTTCTGCAAAGGCAAGTTTCGCACCTCAGCGGAGCGGGAACGCCAC CTGCATATCCTGCACAGGCCCTGGAAGTGCAGCTTGTGCAGTTTTGGCTCCAGCCAGGAGGAGGAGTTGC TGCACCACAGTCTGACGGCCCACGGGGCTTCCGAGCGCCCCCTGGCGGCTACATCTACGCCTGAACCCCC GCCTCCACCCCAGCAAGAACCCAGATCTGCCCTTGAGCCTGAGCCTGAGCCTGAACCTAGGCCAGAGCCT GACCGGGAGGCAAACCCTGCCCCAACTCCTGCACCTCCGGAGGAGCCCCCTGCGCCACCTGAATTCCGTT GCCAGGTGTGCGGCCAGAGCTTTACACAGTCCTGGTTTCTCAAGGGTCACATGCGCAAGCACAAGGCCTC CTTTGATCATGCGTGCCCCGTATGTGGTCGCTGCTTCAAGGAGCCCTGGTTCCTTAAGAACCACATGAAG GTGCACACCAGCAAGCTGGGTCCTTTGCGTGCTCCAGGCCCTGGCTCTGCACCTGCCAGGGCCCCTCAGC CTCCCGACCTGAGCCTACTGGCATATGAGCCACTGGGCCCTGCACTCCTCCTGGCCCCAGCACCCGCTCC GGCTGAGCGCCGAGAGCCCCCAAGCCTCTTAGGCTACCTGAGTGTACGAGCTGGGGAAGTACGACCCAAT GGTGAGGGTGCTGACCCTGGAGGTGGCCGAAGCTATGGAGGGTTCCGCCCACTGCCTTCAGCTCTTCCCA ACCGGGCTCGGCGACACCGTACAGAGGAACCAGAAGAGGAAGAAGAAGTGGTGGAGGCTGAAGAGGAGAG CTGGGCCCGAGGCAGGTCGCTGGGCTCTCTGACTTCCCTGCACCCCAACCCAGGTGAGGGGTCAGGACAG CCTGCACCTGCCGCGGGGACCCAGGCGAGATCCACGGCCACCCAAGAAGAAAACGGGCTGCTGGTTGGAG GGACACGATCTGAAGCGGGCCGTGGGGCCACTGGCAAGGACTGCCCCTTCTGTGGAAAATCTTTCCGCTC GGCGCATCACTTAAAAGTGCATCTCCGTGTGCACACAGGTGAGCGTCCCTACAAGTGTCCACACTGCGAC TATGCAGGTACCCAGTCGGGCTCGCTCAAGTATCACCTTCAGCGTCACCACCGAGAGCAGAGGAGCAGTG CGGGTCCTGGGCCTCCCCCAGAACCCCCGCCACCTTCCCAGCGGGGCTCACTGCAGCCGCAGTCAGGAGC CAAGCCAACTCAGGCCTCAGCCACCTGGGTAGAGGGCACTGCAAGTACCCGGCCTCCTTCGAGCAGCACC GGACCAGGGTCCCGTAGGAAGCCTGCTAGCCCTGGGAGGACCCTGCGAAACGGGCGAGGTGGTGAAGCCG AACCCCTGGACCTGTCCCTACGGGCGGGCCCGGGAGGTGAGGCCGGGGCAGGGGGTGCCCTTCACCGCTG CCTCTTCTGCCCCTTTGCCACTGGAGCTCCTGAGCTCATGGCCTTGCATCTGCAAGTACACCATAGCCGT CGTGCTCGGGGCCGCCGGCAGCCCCGAGCCGACACGTCTCCAACCTATGTCCGGGCACCATCAGGAGAGA CCCCTCCCAGTCCTCCACTAGAAGAGGAGGGCAGCCCAGGGCTGTCTAGATCCGGAGAGGCAGGTCTTGG GGGGCAAGAACGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR230871 representing NM_001253696
Red=Cloning site Green=Tags(s) MGAVGWSETRAGERRFPCPVCGKRFRFNSILALHLRAHPGAQAFQCPHCGHRAAQRALLRSHLRTHQPER PRSPAARLLLELEERALLREARLGRARSSGGMQSSPAAEGLARPQVPSSSAFRCPFCKGKFRTSAERERH LHILHRPWKCSLCSFGSSQEEELLHHSLTAHGASERPLAATSTPEPPPPPQQEPRSALEPEPEPEPRPEP DREANPAPTPAPPEEPPAPPEFRCQVCGQSFTQSWFLKGHMRKHKASFDHACPVCGRCFKEPWFLKNHMK VHTSKLGPLRAPGPGSAPARAPQPPDLSLLAYEPLGPALLLAPAPAPAERREPPSLLGYLSVRAGEVRPN GEGADPGGGRSYGGFRPLPSALPNRARRHRTEEPEEEEEVVEAEEESWARGRSLGSLTSLHPNPGEGSGQ PAPAAGTQARSTATQEENGLLVGGTRSEAGRGATGKDCPFCGKSFRSAHHLKVHLRVHTGERPYKCPHCD YAGTQSGSLKYHLQRHHREQRSSAGPGPPPEPPPPSQRGSLQPQSGAKPTQASATWVEGTASTRPPSSST GPGSRRKPASPGRTLRNGRGGEAEPLDLSLRAGPGGEAGAGGALHRCLFCPFATGAPELMALHLQVHHSR RARGRRQPRADTSPTYVRAPSGETPPSPPLEEEGSPGLSRSGEAGLGGQER TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001253696 |
ORF Size | 2043 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001253696.1, NP_001240625.1 |
RefSeq Size | 2844 bp |
RefSeq ORF | 2046 bp |
Locus ID | 69890 |
MW | 73.7 kDa |
Gene Summary | Transcriptional regulator (PubMed:20940257). Recognizes and binds 2 copies of the core DNA sequence motif 5'-GGGGG-3' (PubMed:20940257). Binds to the HMGN1 promoter and may repress HMGN1 expression (By similarity). Regulates SNCA expression in primary cortical neurons (By similarity). Binds to the COL2A1 promoter and activates COL2A1 expression, as part of a complex with SOX9 (PubMed:20940257). Plays a role in chondrocyte differentiation (PubMed:20940257).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC228660 | Zfp219 (untagged) - Mouse zinc finger protein 219 (Zfp219), transcript variant 4 |
CNY 6,560.00 |