Zfp219 (NM_001253696) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2010302A17Rik; Znf219
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230871 representing NM_001253696 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGCAGTGGGTTGGTCTGAGACTCGTGCAGGCGAACGGCGCTTCCCTTGTCCTGTGTGCGGAAAGC GCTTCCGATTCAATTCCATCTTGGCTTTGCACCTGAGAGCCCACCCGGGCGCCCAAGCCTTCCAGTGCCC ACATTGCGGCCACCGCGCAGCGCAGCGGGCTCTGCTGCGCTCACACCTCCGAACGCACCAGCCGGAGCGT CCACGCAGCCCTGCTGCACGGCTGTTGCTGGAGTTGGAGGAACGCGCCCTGCTTCGCGAAGCCCGACTCG GGAGAGCCAGAAGCTCAGGCGGCATGCAGTCCAGCCCTGCCGCAGAGGGCCTGGCTCGCCCTCAGGTCCC TTCCTCATCTGCCTTCCGTTGCCCTTTCTGCAAAGGCAAGTTTCGCACCTCAGCGGAGCGGGAACGCCAC CTGCATATCCTGCACAGGCCCTGGAAGTGCAGCTTGTGCAGTTTTGGCTCCAGCCAGGAGGAGGAGTTGC TGCACCACAGTCTGACGGCCCACGGGGCTTCCGAGCGCCCCCTGGCGGCTACATCTACGCCTGAACCCCC GCCTCCACCCCAGCAAGAACCCAGATCTGCCCTTGAGCCTGAGCCTGAGCCTGAACCTAGGCCAGAGCCT GACCGGGAGGCAAACCCTGCCCCAACTCCTGCACCTCCGGAGGAGCCCCCTGCGCCACCTGAATTCCGTT GCCAGGTGTGCGGCCAGAGCTTTACACAGTCCTGGTTTCTCAAGGGTCACATGCGCAAGCACAAGGCCTC CTTTGATCATGCGTGCCCCGTATGTGGTCGCTGCTTCAAGGAGCCCTGGTTCCTTAAGAACCACATGAAG GTGCACACCAGCAAGCTGGGTCCTTTGCGTGCTCCAGGCCCTGGCTCTGCACCTGCCAGGGCCCCTCAGC CTCCCGACCTGAGCCTACTGGCATATGAGCCACTGGGCCCTGCACTCCTCCTGGCCCCAGCACCCGCTCC GGCTGAGCGCCGAGAGCCCCCAAGCCTCTTAGGCTACCTGAGTGTACGAGCTGGGGAAGTACGACCCAAT GGTGAGGGTGCTGACCCTGGAGGTGGCCGAAGCTATGGAGGGTTCCGCCCACTGCCTTCAGCTCTTCCCA ACCGGGCTCGGCGACACCGTACAGAGGAACCAGAAGAGGAAGAAGAAGTGGTGGAGGCTGAAGAGGAGAG CTGGGCCCGAGGCAGGTCGCTGGGCTCTCTGACTTCCCTGCACCCCAACCCAGGTGAGGGGTCAGGACAG CCTGCACCTGCCGCGGGGACCCAGGCGAGATCCACGGCCACCCAAGAAGAAAACGGGCTGCTGGTTGGAG GGACACGATCTGAAGCGGGCCGTGGGGCCACTGGCAAGGACTGCCCCTTCTGTGGAAAATCTTTCCGCTC GGCGCATCACTTAAAAGTGCATCTCCGTGTGCACACAGGTGAGCGTCCCTACAAGTGTCCACACTGCGAC TATGCAGGTACCCAGTCGGGCTCGCTCAAGTATCACCTTCAGCGTCACCACCGAGAGCAGAGGAGCAGTG CGGGTCCTGGGCCTCCCCCAGAACCCCCGCCACCTTCCCAGCGGGGCTCACTGCAGCCGCAGTCAGGAGC CAAGCCAACTCAGGCCTCAGCCACCTGGGTAGAGGGCACTGCAAGTACCCGGCCTCCTTCGAGCAGCACC GGACCAGGGTCCCGTAGGAAGCCTGCTAGCCCTGGGAGGACCCTGCGAAACGGGCGAGGTGGTGAAGCCG AACCCCTGGACCTGTCCCTACGGGCGGGCCCGGGAGGTGAGGCCGGGGCAGGGGGTGCCCTTCACCGCTG CCTCTTCTGCCCCTTTGCCACTGGAGCTCCTGAGCTCATGGCCTTGCATCTGCAAGTACACCATAGCCGT CGTGCTCGGGGCCGCCGGCAGCCCCGAGCCGACACGTCTCCAACCTATGTCCGGGCACCATCAGGAGAGA CCCCTCCCAGTCCTCCACTAGAAGAGGAGGGCAGCCCAGGGCTGTCTAGATCCGGAGAGGCAGGTCTTGG GGGGCAAGAACGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230871 representing NM_001253696 Red=Cloning site Green=Tags(s) MGAVGWSETRAGERRFPCPVCGKRFRFNSILALHLRAHPGAQAFQCPHCGHRAAQRALLRSHLRTHQPER PRSPAARLLLELEERALLREARLGRARSSGGMQSSPAAEGLARPQVPSSSAFRCPFCKGKFRTSAERERH LHILHRPWKCSLCSFGSSQEEELLHHSLTAHGASERPLAATSTPEPPPPPQQEPRSALEPEPEPEPRPEP DREANPAPTPAPPEEPPAPPEFRCQVCGQSFTQSWFLKGHMRKHKASFDHACPVCGRCFKEPWFLKNHMK VHTSKLGPLRAPGPGSAPARAPQPPDLSLLAYEPLGPALLLAPAPAPAERREPPSLLGYLSVRAGEVRPN GEGADPGGGRSYGGFRPLPSALPNRARRHRTEEPEEEEEVVEAEEESWARGRSLGSLTSLHPNPGEGSGQ PAPAAGTQARSTATQEENGLLVGGTRSEAGRGATGKDCPFCGKSFRSAHHLKVHLRVHTGERPYKCPHCD YAGTQSGSLKYHLQRHHREQRSSAGPGPPPEPPPPSQRGSLQPQSGAKPTQASATWVEGTASTRPPSSST GPGSRRKPASPGRTLRNGRGGEAEPLDLSLRAGPGGEAGAGGALHRCLFCPFATGAPELMALHLQVHHSR RARGRRQPRADTSPTYVRAPSGETPPSPPLEEEGSPGLSRSGEAGLGGQER TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001253696
ORF Size	2043 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001253696.1, NP_001240625.1
RefSeq Size	2844 bp
RefSeq ORF	2046 bp
Locus ID	69890
MW	73.7 kDa
Gene Summary	Transcriptional regulator (PubMed:20940257). Recognizes and binds 2 copies of the core DNA sequence motif 5'-GGGGG-3' (PubMed:20940257). Binds to the HMGN1 promoter and may repress HMGN1 expression (By similarity). Regulates SNCA expression in primary cortical neurons (By similarity). Binds to the COL2A1 promoter and activates COL2A1 expression, as part of a complex with SOX9 (PubMed:20940257). Plays a role in chondrocyte differentiation (PubMed:20940257).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.