Mapk7 (NM_001291035) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BMK-1; BMK1; ERK-5; ERK5; Erk5-T; PRKM7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230830 representing NM_001291035 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGCGACCTACACCAGATCATTCACTCTTCACAGCCGCTCACCCTGGAACATGTGAGATACTTCC TGTACCAGCTGCTTCGGGGCCTCAAATACATGCACTCTGCTCAGGTCATCCACCGTGATCTTAAACCCTC TAACCTTCTGGTCAATGAGAACTGTGAGCTCAAGATCGGTGACTTTGGAATGGCCCGTGGCCTCTGTACT TCCCCTGCCGAGCACCAGTACTTCATGACTGAGTATGTGGCTACTCGCTGGTACCGTGCCCCGGAGCTCA TGCTTTCCCTGCACGAGTATACGCAGGCAATCGACCTCTGGTCTGTGGGCTGCATCTTTGGTGAGATGCT GGCTCGGCGCCAGCTCTTCCCAGGCAAAAACTACGTGCACCAGTTACAGCTGATCATGATGGTGTTGGGA ACTCCGTCACCAGCTGTGATTCAGGCTGTGGGGGCTGAAAGGGTGCGAGCCTATATCCAGAGCCTGCCAC CAAGGCAACCTGTGCCTTGGGAGACAGTATACCCAGGTGCTGACCGCCAGGCCCTCTCCCTGCTGGGACG CATGTTGCGATTTGAACCCAGTGCCCGAATCTCAGCTGCTGCTGCCCTTCGCCACCCCTTCCTGGCTAAG TACCATGACCCTGATGATGAGCCTGATTGCGCCCCACCTTTTGACTTTGCTTTTGACCGTGAAGCCCTTA CCAGGGAGCGCATTAAGGAGGCCATTGTGGCTGAGATTGAGGACTTCCATGCACGACGGGAGGGCATCCG CCAACAAATCCGCTTCCAGCCTTCTCTGCAGCCTGTGGCTAGTGAGCCTGTGTGTCCAGATGTTGAGATG CCCAGTCCCTGGGCTCCAAGTGGAGACTGTGCCATGGAGTCGCCTCCTCCAGCACTGCCACCATGCTCTG ATCCTGCACCTGACACCGTTGATCTGACTCTGCAGCCTGCCCCGCCGGCCAGTGAGCTTGCTCCACCAAA AAGAGAGGGTGCCATCTCCGACAATACCAAAGCAGCCCTCAAAGCTGCCCTGCTCAAGTCCCTAAGGAGC AGGCTCAGAGATGGGCCCAGTGCACCCTTGGAGGCGCCTGAGCCTCGAAAGCCCGTGACAGCTCAGGAAC GCCAGCGAGAACGAGAAGAGAAGCGCAGGAGGCGACAAGAGAGAGCCAAGGAGCGGGAGAAGCGACGACA AGAGAGAGAACGCAAGGAGAGGGGGGCTGGTACCTTGGGGGGCCCCTCTACTGACCCTCTGGCTGGACTG GTGCTCAGTGACAATGACCGAAGCCTGCTAGAGCGGTGGACTCGCATGGCTAGGCCTCCTGCCCCTGCCC CTGCCCCAGCGCCAGCACCAGCGCCAGCACCGTCCTCTGCCCAGCCCACTAGTACTCCTACTGGCCCCGT ATCTCAGTCTACTGGTCCTCTACAGCCTGCAGGCTCTATTCCGGGTCCTGCCTCCCAGCCTGTTTGCCCA CCCCCTGGCCCTGTTCCCCAGCCTGCTGGCCCTATCCCTGCTCCGCTCCAGACTGCCCCTTCCACTAGCC TTTTGGCCTCCCAGTCACTTGTGCCACCTAGTGGGTTGCCTGGTTCTGGTGCCCCAGAAGTTCTGCCTTA CTTCCCATCTGGCCCACCACCTCCAGATCCTGGGCTCACCCCTCAGCCTTCTACATCAGAGTCACCTGAT GTCAACCTGGTGACTCAGCAGCTGTCCAAGTCTCAGGTGGAGGACCCCCTGCCTCCTGTGTTCTCTGGCA CTCCAAAGGGCAGTGGGGCTGGCTACGGAGTTGGCTTTGATCTGGAGGAATTCTTAAATCAATCTTTTGA TATGGGTGTGGCTGATGGGCCACAGGATGGCCAGGCAGACTCAGCCTCACTCTCAGCCTCTCTCCTTGCT GACTGGCTTGAGGGCCATGGCATGAACCCTGCTGACATTGAGTCTCTGCAGCGTGAGATCCAGATGGACT CCCCAATGCTGCTGTCTGACCTGCCTGACCTCCAAGAGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230830 representing NM_001291035 Red=Cloning site Green=Tags(s) MESDLHQIIHSSQPLTLEHVRYFLYQLLRGLKYMHSAQVIHRDLKPSNLLVNENCELKIGDFGMARGLCT SPAEHQYFMTEYVATRWYRAPELMLSLHEYTQAIDLWSVGCIFGEMLARRQLFPGKNYVHQLQLIMMVLG TPSPAVIQAVGAERVRAYIQSLPPRQPVPWETVYPGADRQALSLLGRMLRFEPSARISAAAALRHPFLAK YHDPDDEPDCAPPFDFAFDREALTRERIKEAIVAEIEDFHARREGIRQQIRFQPSLQPVASEPVCPDVEM PSPWAPSGDCAMESPPPALPPCSDPAPDTVDLTLQPAPPASELAPPKREGAISDNTKAALKAALLKSLRS RLRDGPSAPLEAPEPRKPVTAQERQREREEKRRRRQERAKEREKRRQERERKERGAGTLGGPSTDPLAGL VLSDNDRSLLERWTRMARPPAPAPAPAPAPAPAPSSAQPTSTPTGPVSQSTGPLQPAGSIPGPASQPVCP PPGPVPQPAGPIPAPLQTAPSTSLLASQSLVPPSGLPGSGAPEVLPYFPSGPPPPDPGLTPQPSTSESPD VNLVTQQLSKSQVEDPLPPVFSGTPKGSGAGYGVGFDLEEFLNQSFDMGVADGPQDGQADSASLSASLLA DWLEGHGMNPADIESLQREIQMDSPMLLSDLPDLQEP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291035
ORF Size	2001 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291035.1, NP_001277964.1
RefSeq Size	3265 bp
RefSeq ORF	2004 bp
Locus ID	23939
UniProt ID	Q9WVS8
MW	72.9 kDa
Gene Summary	Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression (By similarity). Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction (By similarity).[UniProtKB/Swiss-Prot Function]

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