Arhgap9 (NM_001285785) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AU043488
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230784 representing NM_001285785 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTCAGGCCGGTGGTGGCCAAGTATCTGGGGAAATCTAGGGCTGAGTCTACAAAGCCCTTCTCAGG GACCCCAGCTCTGCGCCCTCTATCCGTTCACTTACACTGGGGCAGATGGCCGGCAGGTGTCTCTGGCTGA AGGTGACAGGTTCCTACTGCTTCGAAAGACCAACTCAGACTGGTGGCTGGCGAGGCGTCTGGGCGCACCC CCCAGCTCTCGGCCCATCTTTGTTCCAGCTGCATACATGACAGAGGAATCAATCTGCTGCCACAACCCAG CAGACACTACCTACAACAGCTCCCTCTGGACTCCTGGGACAAAGCTGTATCATGGCTCCCTGGAGGAGCT CTATCTGCCTCAGGAACCCCAGGTTGCTTCTGGGCAGCCTCTTCACCATCCCCACAAGATGTGCAAAAGT GTCAGCACGGGCAATGTGAAGGCCTGCTCCCTGAAATCCTTCAAGGAAGGGCCAGGAGAAAGGTCTCACT GGTGGATGACAGAAGTTGCTGTGAAACCGGGCTCCATGGAGCAGACAGAGACTTTGACTAGGGACAGCAG TGCCCTACAGCCTCAGGAATGGCTCGATCCACAGGGTTTACCAAGCCTCAGCCAAAGCGTCCCATGGCTT GCTCTCTCTGAACAGCCGGGAGCCTTGAGGCCCTGTTCAGCTCTGCCACAGCTCCTGGATGACCCCCACG TGGAAGAAAGGTCAGGCCTGCTCAACATGACCAAGATTGCTCAAGGAGGGCGCAAACTCAGGAAGAACTG GGGCCCTGCTTGGGTGGTGTTAACTGGTAGCAGCCTTGTGTTTTACCGAGAGCGGCCACCGCAGTCTGCA TCCCTCCAAGGCTGGGCGCGGGCTGGGAGCCGGCCGGAGAGTAGCGTGGACCTGCGCGGGGCGGCCCTGG CCAGCGGGCGCCAGCTGTCCAGCCGCCGGAACGTCCTGCACATCCGCACGGTCCCGGGACATGAGTTCCT GCTGCAGTCGGACGAGGAGACCGAGCTGCGAGACTGGCACCGCGCGCTGCGGACTGTCATCGAGCGGCTG GATCGGGAGAACCCCCTGGAGCTGCGTCTGTCGGGCTCCGGACCCGCCGAGCTGGCCGAGCTGAGCGCTG GTGAGGATGACGAATTGGAATCGGAACCGGTGTCCAAGTCCCTGATGCGGCTTGGCAGCCGCAGAACTTC CAGTCGGTGTGCAGAGGGCACTGACCAGAAGAACCGCGTGCGGAACAAGTTAAAGCGGCTAATCGCCAAG AGACCAACCTTGCAGAGCCTACAAGAGCGGGGTCTGTTCCGAGACCAGGTTTTTGGCTGCCAGTTGGAGT CGCTGTGCCAACGGGAAGGGGACACCGTGCCCAGCTTTGTCCGGCTTTGTGTTGAGGCCGTGGATAAAAA AGGTCTAGACGTGGATGGTATTTACCGGGTGAGTGGGAACCTGGCCGTGGTCCAGAAACTCCGCTTCCTG GTAGACAGAGAACGGGCAGTGACCTCTGATGGGAGATACATGTTCCCAGAACAGGCAGGACAAGAAGGCA AATTAGATTTAGACAGTGCCGAATGGGATGATATTCACGTGGTCACCGGAGCTTTGAAGCTTTTTTTCCG GGAGCTGCCACAGCCTCTGGTGCCTGCATTGTTGCTGCCTGACTTTCGTGATGCCCTTGAACTTTCTGAG CCAGAGCAGTGCCTCTCCAAAATCCAGAAATTAATAGACTCGCTGCCGAGGCCCAACCATGACACTCTGA AGTACATCCTGGAGCATCTGTGCAGGGTGATAGCACACTCGGATAAGAACCGAATGACCGCCCACAACCT GGGAATTGTGTTTGGACCGACACTGTTTCGACCTGAGCAGGAGGCCTCTGACATGGCGGCCCACGTATTC TACCCCGGGCAGCTGGTCCAGTTGATGCTCAACAACTTTGCCAGCCTCTTCACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230784 representing NM_001285785 Red=Cloning site Green=Tags(s) MFSGRWWPSIWGNLGLSLQSPSQGPQLCALYPFTYTGADGRQVSLAEGDRFLLLRKTNSDWWLARRLGAP PSSRPIFVPAAYMTEESICCHNPADTTYNSSLWTPGTKLYHGSLEELYLPQEPQVASGQPLHHPHKMCKS VSTGNVKACSLKSFKEGPGERSHWWMTEVAVKPGSMEQTETLTRDSSALQPQEWLDPQGLPSLSQSVPWL ALSEQPGALRPCSALPQLLDDPHVEERSGLLNMTKIAQGGRKLRKNWGPAWVVLTGSSLVFYRERPPQSA SLQGWARAGSRPESSVDLRGAALASGRQLSSRRNVLHIRTVPGHEFLLQSDEETELRDWHRALRTVIERL DRENPLELRLSGSGPAELAELSAGEDDELESEPVSKSLMRLGSRRTSSRCAEGTDQKNRVRNKLKRLIAK RPTLQSLQERGLFRDQVFGCQLESLCQREGDTVPSFVRLCVEAVDKKGLDVDGIYRVSGNLAVVQKLRFL VDRERAVTSDGRYMFPEQAGQEGKLDLDSAEWDDIHVVTGALKLFFRELPQPLVPALLLPDFRDALELSE PEQCLSKIQKLIDSLPRPNHDTLKYILEHLCRVIAHSDKNRMTAHNLGIVFGPTLFRPEQEASDMAAHVF YPGQLVQLMLNNFASLFT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001285785
ORF Size	1944 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001285785.2
RefSeq Size	2372 bp
RefSeq ORF	1947 bp
Locus ID	216445
MW	73 kDa

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