Large2 (NM_001290774) Mouse Tagged ORF Clone
CAT#: MR230735
- TrueORF®
Gyltl1b (myc-DDK-tagged) - Mouse glycosyltransferase-like 1B (Gyltl1b), transcript variant 4
"NM_001290774" in other vectors (1)
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CNY 6,270.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 5730485C17Rik; AI891893; Gyltl1b; Largel; mKIAA4105 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR230735 representing NM_001290774
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTGGGCACAACTGCTACCCTCGATGAAGACCCGTACAGGAGTCGCAACCTCTCCGCCTCCAGCCC GCAGCTTCTACTGCCACCCAAGTGCGAGGAAAAATCCGCTGCACCTCCACCTGATAACTGATGCCGTAGC CAGAAACATCCTGGAGACACTCTTCCGAACATGGATGGTGCCAGCGGTGGTGGTCAGCTTCTATGATGCG GAAGAACTCAAGCCCCTGGTCTCCTGGATCCCCAACAAACACTACTCTGGCCTCTATGGGCTAATGAAGC TAGTACTTCCCAGCATCCTGCCTCCCAGCCTGGCCCGAGTCATCGTCCTGGATACCGACGTCACTTTCTC CTCTGACATTGTGGAGCTCTGGGCACTCTTTGATCATTTTTCTGACAAGCAGGTGGTCGGTCTCGTGGAG AACCAGAGCGACTGGTACCTGGGCAACCTCTGGAAGAACCATAGGCCCTGGCCTGCCTTGGGCAGGGGAT TTAACACAGGTGTGATCCTGCTGTGGCTGGACAGGCTCCAGCAAACTGGCTGGGAGCAGATGTGGAAGGT GACAGCCAAACGAGAGCTGCTCACTCTGATGGCTACTTCCTTGGCTGACCAGGACATCTTCAATGCGGTC ATCAAGGAGCACCCCCATCTGGTGCACCCCCTGCCCTGTGTCTGGAACGTGCAGCTGTCAGACCACACTC GGGCTGAGCGCTGCTACCTGGAAGCAGCTGACCTCAAAGTGATCCACTGGAATTCACCAAAGAAGCTTCG AGTGAAGAACAAGCACGCAGAATTCTTCCGTAATCTGCACTTGACCTTTCTGGGGTATGATGGGAAGCTA CTGCGAAGAGAGCTCTTTGGATGCCCCAACCAGTTCCCTCCTGGGGCCGAGCAGTTGCAACAGGCCCTAA CACAGCTGGATGAGGAAGAGCCCTGCTTTGAGTTCCGCCAACAGCAGCTCACTGTGCACCGGGTGCACAT CACCTTCCTGCCCCACCAGCCGCCACCTCCCCAGCCTCACGATGTCACCTTGGTGGCCCAACTCTCTATG GACCGGCTGCAGATGCTGGAAGCCCTGTGCAGGCACTGGCCAGGCCCCATGAGCCTGGCCTTGTACCTGA CAGATGAAGAGGCTCAACAATTTCTTCATTTTGTGGAAACGTCGCCAGTGCTCTCTATGAGGAAGGATGT GGCCTACCATGTAGTGTACCGGGACGGTCCACTCTATCCAGTCAACCAGCTCCGCAACGTGGCCTTGGCC CAGGCTCTCACACCCTACGTCTTCCTCAGTGATATTGACTTCTTACCTGCCTACTCCCTCTACGACTACC TCAGGGCTTCTATCGAGCAGCTGGAGCTGGACAGTCGGCGCAAGACTGCTTTGGTGGTGCCTGCATTTGA GACCCTACACTACCGGTTCAGCTTCCCAAACTCTAAGGCAGAGCTGTTGACGTTACTGGATGCCGGCTCC CTTCACACCTTTAGGTACCACGAGTGGCCACAGGGTCACTCATCCACAGACTATTCCCGCTGGCGGGAAG CCCAGGCACCATACAGTGTGCAGTGGTCAGCTGACTATGAACCCTACGTGGTGGTACCCCGTGACTGCCC CCGTTATGATCCTCGCTTTGTGGGATTTGGCTGGAACAAGGTGGCCCACATCATAGAGTTGGATGCTCAG GAATATGAATTCCTGGTACTTCCTGAGGCCTTCTCTATCCACTTGCCCCACGCTCCAAGTCTTGACATCT CCCGCTTCCGCTCCAGCCCCACCTACCGCAACTGTCTCCAGGCCCTCAAGGAAGAGTTCCACCAGGACTT GTCAAGGCGCTATGGGTCTGCAGCCCTGAAATACCTCACTGCCCTGCAGCAGGCCCGAAGTCGGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR230735 representing NM_001290774
Red=Cloning site Green=Tags(s) MDWAQLLPSMKTRTGVATSPPPARSFYCHPSARKNPLHLHLITDAVARNILETLFRTWMVPAVVVSFYDA EELKPLVSWIPNKHYSGLYGLMKLVLPSILPPSLARVIVLDTDVTFSSDIVELWALFDHFSDKQVVGLVE NQSDWYLGNLWKNHRPWPALGRGFNTGVILLWLDRLQQTGWEQMWKVTAKRELLTLMATSLADQDIFNAV IKEHPHLVHPLPCVWNVQLSDHTRAERCYLEAADLKVIHWNSPKKLRVKNKHAEFFRNLHLTFLGYDGKL LRRELFGCPNQFPPGAEQLQQALTQLDEEEPCFEFRQQQLTVHRVHITFLPHQPPPPQPHDVTLVAQLSM DRLQMLEALCRHWPGPMSLALYLTDEEAQQFLHFVETSPVLSMRKDVAYHVVYRDGPLYPVNQLRNVALA QALTPYVFLSDIDFLPAYSLYDYLRASIEQLELDSRRKTALVVPAFETLHYRFSFPNSKAELLTLLDAGS LHTFRYHEWPQGHSSTDYSRWREAQAPYSVQWSADYEPYVVVPRDCPRYDPRFVGFGWNKVAHIIELDAQ EYEFLVLPEAFSIHLPHAPSLDISRFRSSPTYRNCLQALKEEFHQDLSRRYGSAALKYLTALQQARSRA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001290774 |
ORF Size | 1887 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001290774.1, NP_001277703.1 |
RefSeq Size | 2370 bp |
RefSeq ORF | 1890 bp |
Locus ID | 228366 |
UniProt ID | Q5XPT3 |
MW | 73.2 kDa |
Gene Summary | Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Has a higher activity toward alpha-dystroglycan than LARGE (PubMed:15958417).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC228524 | Gyltl1b (untagged) - Mouse glycosyltransferase-like 1B (Gyltl1b), transcript variant 4 |
CNY 6,180.00 |