Ebf2 (NM_001276387) Mouse Tagged ORF Clone
CAT#: MR230561
- TrueORF®
Ebf2 (myc-DDK-tagged) - Mouse early B cell factor 2 (Ebf2), transcript variant 1
"NM_001276387" in other vectors (1)
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CNY 4,280.00
CNY 5,700.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | D14Ggc1e; EBF-2; Mmot1; O/E-3; OE-3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR230561 representing NM_001276387
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGGGATTCAAGATACGCTAGGAAGAGGACCAGCTCTGAAAGACAAGTCGCTGGGTGCCGAGATGG ATTCGGTCAGGTCCTGGGTCCGGAATGTCGGCGTGGTGGACGCCAATGTCGCGGCTCAGAGCGGAGTTGC TCTGTCCCGGGCCCATTTTGAAAAGCAACCTCCTTCAAACTTGAGGAAATCCAACTTCTTTCATTTTGTC CTGGCGCTCTACGACAGGCAGGGACAGCCGGTGGAGATTGAGCGCACAGCCTTCGTGGACTTTGTGGAGA ATGACAAAGAGCAAGGCAATGAGAAGACCAACAATGGTACTCACTACAAGTTACAGCTCCTTTACAGCAA CGGTGTCCGCACGGAGCAGGACCTGTATGTCAGGCTCATCGACTCTGTCACCAAACAGCCCATAGCCTAT GAGGGACAGAATAAGAATCCCGAAATGTGCAGAGTTCTGCTGACACACGAGGTGATGTGCAGTCGATGCT GTGAGAAGAAGAGCTGCGGGAACCGGAACGAGACCCCTTCAGACCCGGTCATCATCGACAGATTCTTTTT AAAATTTTTCCTGAAGTGCAATCAGAATTGTTTGAAAACAGCGGGAAACCCAAGGGACATGAGGCGGTTC CAGGTCGTGTTGTCCACAACAGTAAATGTGGATGGACATGTGCTGGCTGTTTCTGACAACATGTTTGTTC ATAACAATTCTAAGCATGGAAGGAGAGCAAGAAGGCTTGACCCATCTGAAGCTACACCCTGCATCAAAGC CATCAGCCCAAGTGAAGGCTGGACCACAGGAGGGGCCATGGTCATCATCATTGGTGACAACTTTTTTGAT GGTCTCCAGGTTGTGTTTGGTACCATGCTTGTATGGAGTGAGCTCATAACCCCTCATGCCATCAGAGTAC AGACCCCTCCCCGGCATATCCCTGGAGTTGTGGAAGTGACACTATCTTATAAATCCAAACAGTTCTGCAA AGGAGCCCCGGGCAGGTTCATCTACACAGCCTTAAATGAACCCACCATAGACTATGGCTTCCAAAGATTG CAGAAAGTCATCCCAAGGCACCCAGGTGACCCTGAGAGGCTAGCTAAGGAAATGCTGTTGAAAAGAGCTG CGGACCTGGTGGAAGCCCTCTACGGCACACCACACAATAATCAGGACATCATTTTAAAGCGTGCTGCAGA CATTGCTGAAGCCCTGTACAGTGTCCCCAGGAACCCCAGCCAGATTCCAGCTCTTTCCAGCTCCCCAGCT CACAGCGGTATGATGGGTATCAACTCTTACGGCAGCCAGCTCGGCGTCAGCATTTCAGAGTCCACACAAG GAAATAACCAAGGATACATCCGCAACACAAGCAGCATCTCCCCGCGGGGATACTCCTCCAGCTCCACGCC TCAGCAATCTAATTACAGCACCTCCAGCAACAGCATGAACGGCTACAGCAATGTTCCTATGGCCAACCTG GGTGTGCCAGGATCCCCGGGATTTCTGAATGGCTCACCCACGGGCTCCCCATATGGAATCATGTCTTCAA GCCCCACCGTGGGATCCTCCAGCACGTCCTCCATCCTCCCGTTCTCCTCTTCAGTTTTTCCTGCTGTCAA ACAGAAGAGTGCCTTTGCCCCTGTCATCAGGCCCCAAGGCTCCCCATCGCCTGCCTGCTCCAGCGGCAAT GGGAACGGATTCAGAGCCATGACAGGACTTGTTGTTCCCCCGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR230561 representing NM_001276387
Red=Cloning site Green=Tags(s) MFGIQDTLGRGPALKDKSLGAEMDSVRSWVRNVGVVDANVAAQSGVALSRAHFEKQPPSNLRKSNFFHFV LALYDRQGQPVEIERTAFVDFVENDKEQGNEKTNNGTHYKLQLLYSNGVRTEQDLYVRLIDSVTKQPIAY EGQNKNPEMCRVLLTHEVMCSRCCEKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKTAGNPRDMRRF QVVLSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEATPCIKAISPSEGWTTGGAMVIIIGDNFFD GLQVVFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGAPGRFIYTALNEPTIDYGFQRL QKVIPRHPGDPERLAKEMLLKRAADLVEALYGTPHNNQDIILKRAADIAEALYSVPRNPSQIPALSSSPA HSGMMGINSYGSQLGVSISESTQGNNQGYIRNTSSISPRGYSSSSTPQQSNYSTSSNSMNGYSNVPMANL GVPGSPGFLNGSPTGSPYGIMSSSPTVGSSSTSSILPFSSSVFPAVKQKSAFAPVIRPQGSPSPACSSGN GNGFRAMTGLVVPPM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001276387 |
ORF Size | 1725 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001276387.1, NP_001263316.1 |
RefSeq Size | 5532 bp |
RefSeq ORF | 1728 bp |
Locus ID | 13592 |
UniProt ID | O08792 |
MW | 62.6 kDa |
Gene Summary | Transcription factor that, in osteoblasts, activates the decoy receptor for RANKL, TNFRSF11B, which in turn regulates osteoclast differentiation. Acts in synergy with the Wnt-responsive LEF1/CTNNB1 pathway. Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC228350 | Ebf2 (untagged) - Mouse early B cell factor 2 (Ebf2), transcript variant 1 |
CNY 5,610.00 |