St7 (NM_001289625) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	9430001H04Rik; Fam4a2; HELG; RAY1; SEN4; TSG7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230552 representing NM_001289625 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGGCGGGCGCGGGCTTCCTGGAGCAGCTCAAGTCCTGCATCGTGTGGTCGTGGACCTATCTGT GGACCGTGTGGTTCTTCCTCGTGCTCTTCCTGGTCTACATCCTGAGGGTGCCCCTGAGGATCAACGACAA CCTGAGCACAGTGAGCATGTTTTTGAACACGTTAACCCCAAAGTTCTACGTGGCCCTGACAGGCACTTCC TCGCTAATATCGGGACTTATTTTGATATTTGAATGGTGGTATTTTCGCAAGTATGGAACATCTTTCATTG AACAAGTCTCCGTAAGCCACTTGCGCCCCCTTCTGGGAGGGGTTGACAACAACTCTTCCAATAATTCTAA TTCCAGTAATGGAGACTCAGATTCCAACAGACAAAGTGTCTCAGAATGCAAAGTATGGCGAAATCCACTG AATCTGTTTAGGGGTGCGGAATATAATCGGTACACTTGGGTCACAGGACGAGAGCCTCTGACCTACTATG ACATGAACCTCTCTGCCCAGGACCACCAGACATTCTTTACTTGTGATTCAGATCATCTTCGCCCTGCAGA TGCAATAATGCAAAAAGCTTGGAGAGAAAGGAACCCTCAAGCCAGGATTTCTGCAGCTCATGAAGCCTTG GAGATAAACGAAATTAGGTCCAGAGTTGAAGTTCCCCTAATTGCTTCCTCTACCATCTGGGAGATAAAAT TGTTACCAAAGTGTGCAACTGCGTACATTCTCTTGGCGGAAGAAGAAGCGACAACTATTGCTGAAGCAGA AAAACTCTTTAAGCAGGCCCTGAAGGCTGGAGATGGTTGTTACCGGCGCTCTCAGCAGCTACAACATCAT GGGTCCCAGTATGAAGCTCAACACAGACGAGACACCAATGTCTTAGTCTATATCAAAAGAAGGCTGGCGA TGTGTGCCCGGAGACTTGGAAGGACCAGAGAAGCAGTGAAGATGATGAGAGATTTAATGAAGGAGTTTCC CCTCCTAAGCATGTTCAATATCCATGAGAACCTTCTAGAAGCTCTTCTGGAACTCCAAGCTTATGCTGAT GTTCAGGCAGTCTTAGCAAAGTATGATGATATAAGCTTACCAAAGTCGGCGACAATATGCTACACAGCCG CCCTGCTCAAAGCAAGAGCTGTCTCTGACAAATTCTCTCCAGAGGCTGCGTCTCGGAGGGGGCTGAGCAC AGCAGAGATGAATGCAGTAGAAGCCATCCACAGAGCTGTGGAATTTAATCCACACGTGCCAAAATATCTA CTAGAAATGAAAAGCTTAATCCTCCCACCAGAACACATCCTGAAGAGAGGAGACAGTGAAGCGATAGCAT ATGCATTCTTTCATCTTGCACACTGGAAGAGGGTGGAAGGGGCTTTGAATCTCTTGCATTCTTTCCGGAT GATCCCGTATCCCCTGGAGAAGGGACACCTATTTTATCCATACCCAATCTGTACAGAAACAGCTGACCGG GAGCTGCTTCCCTCTTTCCATGAAGTCTCAGTTTACCCAAAGAAGGAACTTCCCTTCTTCATCCTCTTCA CTGCTGGACTGTGCTCCTTCACAGCCATGCTGGCCCTCCTGACACATCAGTTTCCGGAACTTATGGGAGT CTTCGCAAAAGCTTTCCTCAGCACTTTGTTTGCCCCCTTGAACTTTGTTATGGAGAAAGTGGAAAGCATC CTCCCCTCCAGTTTGTGGCATCAGCTGACACGGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230552 representing NM_001289625 Red=Cloning site Green=Tags(s) MAEAGAGFLEQLKSCIVWSWTYLWTVWFFLVLFLVYILRVPLRINDNLSTVSMFLNTLTPKFYVALTGTS SLISGLILIFEWWYFRKYGTSFIEQVSVSHLRPLLGGVDNNSSNNSNSSNGDSDSNRQSVSECKVWRNPL NLFRGAEYNRYTWVTGREPLTYYDMNLSAQDHQTFFTCDSDHLRPADAIMQKAWRERNPQARISAAHEAL EINEIRSRVEVPLIASSTIWEIKLLPKCATAYILLAEEEATTIAEAEKLFKQALKAGDGCYRRSQQLQHH GSQYEAQHRRDTNVLVYIKRRLAMCARRLGRTREAVKMMRDLMKEFPLLSMFNIHENLLEALLELQAYAD VQAVLAKYDDISLPKSATICYTAALLKARAVSDKFSPEAASRRGLSTAEMNAVEAIHRAVEFNPHVPKYL LEMKSLILPPEHILKRGDSEAIAYAFFHLAHWKRVEGALNLLHSFRMIPYPLEKGHLFYPYPICTETADR ELLPSFHEVSVYPKKELPFFILFTAGLCSFTAMLALLTHQFPELMGVFAKAFLSTLFAPLNFVMEKVESI LPSSLWHQLTRI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289625
ORF Size	1716 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289625.1, NP_001276554.1
RefSeq Size	2195 bp
RefSeq ORF	1719 bp
Locus ID	64213
UniProt ID	Q99M96
MW	66 kDa

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