Elf2 (NM_001291063) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2610036A20Rik; A230104O07Rik; AW111824; BB183398; EU32; NERF; NERF-1A; NERF-1B; NERF-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230419 representing NM_001291063 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACGTCTCTGCATGAGGGACCCACGAACCAGCTGGATCTGCTCATCCGGGCCGTGGAAGCATCAG TTCACAGCAGTAATGCTCACTGTACGGATAAGACCATTGAAGCTGCTGAAGCCCTGCTTCATATGGAATC TCCTACCTGCTTGAGGGATTCAAGAAGTCCTGTGGAAGTGTTTGTCCCTCCTTGTATATCAACTCCAGAG TTTATCCATGCTGCGATGAGGCCGGATGTCATCACAGAGACTGTAGTGGAGGTGTCCACAGAAGAGTCTG AGCCGATGGATGCCTCGCCTATCCCTACTTCACCAGACAGCCACGAACCAATGAAAAAGAAAAAAGTTGG CCGTAAACCAAAGACCCAGCAGTCACCAGTTTCCAATGGGTCTCCTGAGTTAGGAATCAAGAAGAAAGCC AGAGAAGGAAAAGGAAACACAACCTATCTGTGGGAGTTTCTTTTAGATTTGCTTCAAGATAAAAATACTT GTCCCAGGTATATTAAATGGACTCAGAGAGAAAAGGGCATATTCAAACTTGTGGACTCAAAGGCTGTCTC GAAGCTCTGGGGGAAGCATAAGAACAAACCAGATATGAACTATGAAACCATGGGGCGAGCGTTAAGATAT TACTACCAAAGGGGAATTCTTGCAAAGGTTGAAGGACAGAGGCTTGTATATCAGTTCAAGGACATGCCCA AAAACATAGTGGTCATTGATGATGACAAAAGTGAGACCTGCCCTGAAGACTTGGCAGCAGCTGCTGATGA CAAGTCTTTAGAACGAGTGTCACTATCTGCAGAAAGTCTCCTGAAGGCAGCAACTGCCGTCCGAGGTGGG AAAAACTCATCTCCTCTGAACTGCTCCAGAGCAGAGAAGGGTGTGGCTAGAGTTGTGAACATCACCTCCC CTACTCACGACGGTTCCTCCAGGTCTCCTACTACCACTGCACCTGTGTCAGCAGCAGCAGCTCCAAGGAC AGTTCGTGTGGCAATGCAAGTCCCTGTGGTAATGACCTCACTGGGCCAAAAGATTTCAGCGGTGGCAGTT CAGTCAGTCAATGCAGGCACAGGCTCGCCGTTAATAACCAGCACCAGTCCAGCCTCGGCCAGCTCTCCAA AAGTAGTCATCCAGACAGTCCCGACCGTGATGCCGGCCTCCACTGAAAATGGAGACAGAATCACCATGCA GCCTGCCAAGATTATCACCATCCCCGCCACCCAGCTCGCACAGTGTCAACTCCAGGCAAAGTCAAACCTG ACGGGGTCAGGAAGCATTAACATTGTTGGAACCCCACTGGCTGTGAGGGCACTCACCCCTGTTTCAATAG CCCACGGTACACCTGTAATGAGACTGTCTGTGCCTGCTCAGCAGGCTTCTGGCCAGACTCCTCCTCGAGT TATCAGTGCGCTCCTAAAAGGGCCGGAGGGGAAATCAGAAGCCAAAAAGCAGGAACACGATGTGAAAACT TTGCAGCTGGTAGAAGAGAAGGGGGCAGACGGCAATAAGACAGTAACCCACGTAGTGGTCGTCAGTGCGC CGTCTGCTATCGCCCTTCCTGTGACTATGAAAACCGAAGGGCTAGTGACGTGTGAGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230419 representing NM_001291063 Red=Cloning site Green=Tags(s) MATSLHEGPTNQLDLLIRAVEASVHSSNAHCTDKTIEAAEALLHMESPTCLRDSRSPVEVFVPPCISTPE FIHAAMRPDVITETVVEVSTEESEPMDASPIPTSPDSHEPMKKKKVGRKPKTQQSPVSNGSPELGIKKKA REGKGNTTYLWEFLLDLLQDKNTCPRYIKWTQREKGIFKLVDSKAVSKLWGKHKNKPDMNYETMGRALRY YYQRGILAKVEGQRLVYQFKDMPKNIVVIDDDKSETCPEDLAAAADDKSLERVSLSAESLLKAATAVRGG KNSSPLNCSRAEKGVARVVNITSPTHDGSSRSPTTTAPVSAAAAPRTVRVAMQVPVVMTSLGQKISAVAV QSVNAGTGSPLITSTSPASASSPKVVIQTVPTVMPASTENGDRITMQPAKIITIPATQLAQCQLQAKSNL TGSGSINIVGTPLAVRALTPVSIAHGTPVMRLSVPAQQASGQTPPRVISALLKGPEGKSEAKKQEHDVKT LQLVEEKGADGNKTVTHVVVVSAPSAIALPVTMKTEGLVTCEK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291063
ORF Size	1599 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291063.1, NP_001277992.1
RefSeq Size	5495 bp
RefSeq ORF	1602 bp
Locus ID	69257
UniProt ID	Q9JHC9
MW	57.5 kDa
Gene Summary	Probably transcriptionally activates the LYN and BLK promoters and acts synergistically with RUNX1 to transactivate the BLK promoter.[UniProtKB/Swiss-Prot Function]

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