Chfr (NM_001289579) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	5730484M20Rik; C230082M18; RNF116
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230380 representing NM_001289579 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCATGTGACCAAAGATTGCTCAGGTCCAGGGCAGGGTGATGATCCCCAGGTTCCACTATTGTCAC CCATGGCTCAGACATGCTTAGAGGAACCACAGCCATCAACATCGACATCAGACCTCCTCCCCACGGCCTC TACCTCTTCTACGGAGCCAGAGCTGACCTCTGCAGGGCAAAAGCATTCTTCTAGCTCTGGACCTGGGAAC ACAAGCATCTCCCCAAAAGGACGCAGTTCACTTGTTGCAAATGGCGAACTCTCTAGCCTTTCTCCAGTTT TCCAAGACAAAGAAGCATCCTTTTCTTTGCTGGAAAGTAAAGACCATGAGGAATTGGAGCCTGCCAAAAA AAAGATGAAAGGAGATGGGGAACTTGACACGAACCTCCAGTTATTAGTTTCAGGCCAGCGTGGAAATGCC CAAACCTCAAGTGAAGATGTCAAAGATGCCTCTGTGAAGCCAGACAAGATGGAGGAGACACTAACCTGTA TCATCTGCCAGGACCTTCTGCACGATTGTGTGAGTTTGCAGCCTTGTATGCACACATTTTGTGCGGCTTG CTACTCTGGTTGGATGGAGCGTTCATCTCTGTGCCCTACCTGCCGATGTCCAGTGGAGCGGATTTGCAAA AACCACATCCTGAACAACCTAGTGGAAGCATACCTTATCCAGCACCCAGATAAAAGTCGCAGTGAAGAAG ATGTGAGAAGTATGGATGCAAGGAATAAAATCACTCAAGATATGCTGCAACCCAAAGTCAGGAGGTCTTT CTCTGATGAAGAGGGGAGTTCAGAGGACCTGCTAGAGCTGTCTGATGTCGACAGTGAATCCTCAGATATC AGTCAGCCATACATTGTCTGCAGACAGTGTCCTGAATACAGAAGGCAAGCGGTGCAGTCTCTTCCTTGCC CAGTCCCAGAGAGTGAGCTGGGAGCTACACTGGCCCTTGGTGGGGAGGCACCTTCAACATCTGCCAGCTT GCCAACAGCAGCCCCGGATTACATGTGCCCTCTTCAAGGAAGCCATGCCATATGCACCTGCTGCTTCCAG CCTATGCCTGACCGGAGAGCTGAACGGGAGCAGGATCCCCGCGTCGCCCCTCAGCAGTGTGCGGTGTGCC TGCAGCCCTTCTGCCACCTGTACTGGGGCTGCACGAGGACTGGCTGCTTTGGCTGCTTGGCCCCATTCTG TGAGCTCAACCTGGGGGACAAGTGCTTGGATGGAGTGCTGAACAATAACAATTATGAATCGGACATCCTG AAGAATTACCTGGCAACCAGGGGTCTGACATGGAAAAGTGTGTTGACAGAGAGTCTCCTGGCTCTGCAGC GAGGTGTATTTATGCTGTCTGATTACAGAATCACTGGAAATACTGTGCTGTGTTACTGCTGTGGTCTGCG TAGCTTCCGAGAGCTGACCTACCAGTATCGTCAGAACATTCCTGCTTCTGAGTTGCCAGTGACTGTAACA TCCCGTCCTGACTGCTACTGGGGCCGTAACTGTCGCACTCAGGTGAAGGCTCACCATGCAATGAAATTCA ATCACATCTGTGAGCAAACAAGGTTCAAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230380 representing NM_001289579 Red=Cloning site Green=Tags(s) MFHVTKDCSGPGQGDDPQVPLLSPMAQTCLEEPQPSTSTSDLLPTASTSSTEPELTSAGQKHSSSSGPGN TSISPKGRSSLVANGELSSLSPVFQDKEASFSLLESKDHEELEPAKKKMKGDGELDTNLQLLVSGQRGNA QTSSEDVKDASVKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICK NHILNNLVEAYLIQHPDKSRSEEDVRSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDI SQPYIVCRQCPEYRRQAVQSLPCPVPESELGATLALGGEAPSTSASLPTAAPDYMCPLQGSHAICTCCFQ PMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDIL KNYLATRGLTWKSVLTESLLALQRGVFMLSDYRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPVTVT SRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289579
ORF Size	1572 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289579.1, NP_001276508.1
RefSeq Size	3253 bp
RefSeq ORF	1575 bp
Locus ID	231600
UniProt ID	Q810L3
MW	58.4 kDa
Gene Summary	E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress (By similarity).[UniProtKB/Swiss-Prot Function]

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