Etv3 (NM_001286844) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI414410; METS; Pe1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230330 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGCAGGCTGTAGCATCGTGGAAAAGCCAGAAGGAGGTGGAGGGTATCAGTTTCCGGATTGGGCCT ACAAAGCCGAGTCGTCGCCGGGCTCCCGGCAGATCCAGCTGTGGCACTTCATCCTGGAACTGCTGCAGAA GGAAGAGTTCCGCCATGTCATCGCCTGGCAGCAGGGAGAGTACGGGGAGTTTGTCATCAAGGATCCAGAT GAAGTGGCTCGCCTCTGGGGCCGCAGGAAGTGCAAACCACAGATGAACTATGACAAGCTGAGCCGGGCCC TCAGATACTATTACAACAAGAGGATCCTTCATAAAACAAAAGGGAAAAGGTTTACTTACAAGTTTAACTT CAACAAGCTCGTGATGCCCAACTACCCGTTCATCAACATTCGCTCGAGTGGTGTGGTTCCTCAGAGTGCG CCACCAGTGCCAACAGCCTCCTCCCGGTTCCATTTCCCACCTCTAGACAGCCATTCTCCTACTGGCGATG TGCAACCGGGTCGCTTCTCTGCCAGCTCACTGAGTGCTTCTGGCCCTGAGTCAGGGGTTACCACTGACAG GAAGGTCGAGCCTTCGGACCTGGAAGATGGCTCGGCCTCTGACTGGCACCGGGGCATGGACTTCATGCCC TCCCGAAATGCTCTGGGCGGAGGAGCAGTCGGCCACCAGAAACGCAAGCCTGACATACTGCTTCCTCTCT TCACCCGGCCAGCCATGTACCCTGACCCGCACAGTCCCTTCGCTATCTCTCCGGTCCCTGGCCGCGGAGG GGTCCTTAATGTCCCCATCTCACCAGCCCTGTCCCTGACTCCCACCATGTTCTCCTACAGTCCCTCACCA GGCCTGAGCCCCTTCACCAGCAGCAGTTGCTTCTCCTTCAACCCAGAGGAAATGAAACACTACCTTCATT CTCAAGCCTGTTCGGTGTTCAACTACCATCTGAGTCCCCGGACTTTCCCCCGTTACCCAGGGCTCATGGT CCCACCGCTGCAGTGCCAAATGCATCCTGAGGAGCCTTCCCAGTTCTCCATCAAGCTGCAGCCCCCGCCG GCTGGACGGAAGAACCGCGAGAGGGTAGAGAGCCGGGAGGAGGCTGTTCGCGGCTCTGTGCCCGCCAGCG CTCCTGTTCCCTCTCGGATTAAGGTAGAGCCGGCCACAGAGAAGGATCCTGACAGCCTCCGGCAGTCAAC CCAGGGAAAGGAGGAACAGACCCAAGAAGTGGACAGCATGCGGAGCAGGACCATAGAAGAGGGGAAAGGC ACCGGGTTTGCCCACCCCTCACCCACCTGGCCCTCTGTGTCCATTAGCACTCCCAGTGACGAACCCCTAG AGGGGACCGAAGACAGTGAGGACAGGTCTGTCAGGGAGCCCGGTGTACCCGAGAAGAAAGAAGACGCCCT GATGCCCCCTAAGCTTCGGCTGAAGAGGCGGTGGAATGACGACCCTGAAGCCAGGGAGCTCAACAAAACG GGCAAGTTCCTCTGGAATGGGGCAGGACCCCAGGGCCTGGCCACAACGGCCACTGCTGCCGCTGATGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230330 protein sequence Red=Cloning site Green=Tags(s) MKAGCSIVEKPEGGGGYQFPDWAYKAESSPGSRQIQLWHFILELLQKEEFRHVIAWQQGEYGEFVIKDPD EVARLWGRRKCKPQMNYDKLSRALRYYYNKRILHKTKGKRFTYKFNFNKLVMPNYPFINIRSSGVVPQSA PPVPTASSRFHFPPLDSHSPTGDVQPGRFSASSLSASGPESGVTTDRKVEPSDLEDGSASDWHRGMDFMP SRNALGGGAVGHQKRKPDILLPLFTRPAMYPDPHSPFAISPVPGRGGVLNVPISPALSLTPTMFSYSPSP GLSPFTSSSCFSFNPEEMKHYLHSQACSVFNYHLSPRTFPRYPGLMVPPLQCQMHPEEPSQFSIKLQPPP AGRKNRERVESREEAVRGSVPASAPVPSRIKVEPATEKDPDSLRQSTQGKEEQTQEVDSMRSRTIEEGKG TGFAHPSPTWPSVSISTPSDEPLEGTEDSEDRSVREPGVPEKKEDALMPPKLRLKRRWNDDPEARELNKT GKFLWNGAGPQGLATTATAAADA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286844
ORF Size	1539 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286844.1, NP_001273773.1
RefSeq Size	5459 bp
RefSeq ORF	1542 bp
Locus ID	27049
UniProt ID	Q8R4Z4
MW	57 kDa
Gene Summary	Transcriptional repressor that contribute to growth arrest during terminal macrophage differentiation by repressing target genes involved in Ras-dependent proliferation. Represses MMP1 promoter activity.[UniProtKB/Swiss-Prot Function]

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