Tfe3 (NM_001271490) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	bHLHe33; F830016E06Rik; Tcfe3; Tfe-3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230060 representing NM_001271490 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATCTTCTTCATCACGGGTCTTGCTGCGTCAGCAGCTTATGAGGGCCCAGGCTCAGGAACAGGAGA GGCGTGAGCGGCGGGAACAGGCAGCGGCCGCTCCCTTCCCCAGCCCTGCGCCCGCCTCACCAGCCATCTC TGTGATTGGCGTGTCTGCTGGTGGCCACACACTGAGTCGTCCACCTCCTGCTCAGGTGCCCAGGGAGGTA CTCAAGGTTCAGACCCACCTCGAGAACCCTACACGCTACCACCTGCAGCAAGCTCGCCGGCAGCAGGTCA AACAGTACTTGTCTACCACACTTGGGCCCAAGCTGGCTTCCCAGGCTCTCACCCCACCACCAGGGCCTTC CAGTGCCCAGCCACTTCCTGCCCCTGAAACTGCTCATGCCACCGGTCCTACAGGCAGTGCTCCTAACAGC CCCATGGCGCTGCTCACCATTGGGTCCAGCTCAGAAAAGGAGATTGATGATGTCATTGATGAGATCATCA GCCTGGAGTCCAGTTACAACGATGAGATGCTCAGCTATCTTCCAGGAGGCACTGCAGGGCTGCAGCTCCC CAGCACGCTGCCGGTGTCTGGAAATCTACTTGATGTGTACAGCAGTCAGGGAGTGGCTACCCCAGCTATC ACCGTCAGCAATTCCTGTCCAGCTGAGCTGCCTAACATCAAACGCGAGATCTCCGAAACCGAGGCAAAGG CCCTTTTGAAGGAGCGACAGAAGAAAGACAATCACAACCTAATTGAACGACGCAGGCGATTCAACATTAA CGATAGGATCAAAGAGCTGGGCACCCTCATCCCCAAGTCCAATGATCCGGAGATGCGCTGGAACAAGGGC ACCATCCTGAAGGCATCTGTGGATTACATCCGCAAATTACAGAAGGAACAGCAACGCTCCAAAGACCTGG AGAGCCGGCAGCGGTCCCTGGAACAAGCCAACCGAAGCCTGCAGCTCCGGATTCAGGAGCTAGAACTGCA GGCCCAGATCCATGGTCTGCCAGTCCCTCCCAACCCAGGACTGCTCTCCCTAACCACGAGTTCGGTCTCT GACAGCCTCAAGCCAGAACAGCTGGACATTGAGGAGGAGGGCAGGCCTAGCACAACGTTCCATGTATCGG GAGGACCTGCCCAGAACGCTCCTCCACAACAGCCTCCAGCACCACCTTCGGATGCCCTTCTGGACCTACA CTTTCCCAGCGACCACTTGGGAGACCTGGGGGACCCCTTCCACCTAGGGCTAGAGGACATTCTGATGGAG GAGGAGGGGATGGTGGGAGGACTGTCAGGGGGTGCCCTGTCCCCACTGCGGGCTGCTTCTGACCCCCTGC TTTCTTCAGTATCCCCGGCTGTTTCCAAGGCCAGCAGCCGCCGTAGCAGCTTCAGCATGGAAGAGGAGTC C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230060 representing NM_001271490 Red=Cloning site Green=Tags(s) MSSSSSRVLLRQQLMRAQAQEQERRERREQAAAAPFPSPAPASPAISVIGVSAGGHTLSRPPPAQVPREV LKVQTHLENPTRYHLQQARRQQVKQYLSTTLGPKLASQALTPPPGPSSAQPLPAPETAHATGPTGSAPNS PMALLTIGSSSEKEIDDVIDEIISLESSYNDEMLSYLPGGTAGLQLPSTLPVSGNLLDVYSSQGVATPAI TVSNSCPAELPNIKREISETEAKALLKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPEMRWNKG TILKASVDYIRKLQKEQQRSKDLESRQRSLEQANRSLQLRIQELELQAQIHGLPVPPNPGLLSLTTSSVS DSLKPEQLDIEEEGRPSTTFHVSGGPAQNAPPQQPPAPPSDALLDLHFPSDHLGDLGDPFHLGLEDILME EEGMVGGLSGGALSPLRAASDPLLSSVSPAVSKASSRRSSFSMEEES TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271490
ORF Size	1401 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271490.1, NP_001258419.1
RefSeq Size	3446 bp
RefSeq ORF	1404 bp
Locus ID	209446
UniProt ID	Q64092
MW	51 kDa
Gene Summary	Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3') (PubMed:16936731). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF (PubMed:16936731). In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity (PubMed:16936731). Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer (By similarity). It also binds very well to a USF/MLTF site (By similarity). May regulate lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 (PubMed:29146937).[UniProtKB/Swiss-Prot Function]

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