Mpl (NM_001285497) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	c-mpl; CD110; hlb219; TPO-R
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230049 representing NM_001285497 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTCTTGGGCCCTCTTCATGGTCACCTCCTGCCTCCTCTTGGCCCTTCCAAACCAGGCACAAGTCA CCAGCCAAGATGTCTTCTTGCTGGCCTTGGGCACAGAGCCCCTGAACTGCTTCTCCCAAACATTTGAGGA CCTCACCTGCTTCTGGGATGAGGAAGAGGCAGCACCCAGTGGGACATACCAGCTGCTGTATGCCTACCGA GGAGAGAAGCCCCGTGCATGCCCCCTGTATTCCCAGAGTGTGCCCACCTTTGGAACCCGGTATGTGTGCC AGTTTCCAGCCCAGGATGAAGTGCGCCTCTTCTTTCCGCTGCACCTCTGGGTGAAGAATGTGTCCCTCAA CCAGACTTTGATCCAGCGGGTGCTGTTTGTGGATAGTGTGGGCCTGCCAGCTCCCCCCAGGGTCATCAAG GCCAGGGGTGGGAGCCAACCAGGGGAACTTCAGATCCACTGGGAGGCCCCTGCTCCTGAAATCAGTGACT TCCTGAGGCATGAACTCCGCTATGGCCCCACGGACTCCAGCAACGCCACTGCCCCCTCCGTCATTCAGCT GCTCTCCACAGAAACCTGCTGCCCCACTTTGTGGATGCCGAACCCAGTCCCTGTTCTTGACCAGCCTCCG TGTGTTCATCCGACAGCATCCCAACCGCATGGACCAGTGAGGACCTCCCCAGCTGGAGAAGCTCCATTTC TGACAGTGAAGGGTGGAAGCTGTCTCGTCTCAGGCCTCCAGGCTGGCAAATCCTACTGGCTCCAGCTACG CAGCCAACCCGACGGGGTCTCCCTTCGTGGCTCCTGGGGACCCTGGTCCTTCCCTGTGACTGTGGATCTT CCAGGAGATGCAGTGACAATTGGACTTCAGTGCTTTACCTTGGATCTGAAGATGGTCACCTGCCAGTGGC AGCAACAAGACCGCACTAGCTCCCAAGGCTTCTTCCGCCACAGCAGGACGAGGTGCTGCCCCACAGACAG GGACCCCACCTGGGAGAAATGTGAAGAGGAGGAACCGCGTCCAGGATCACAGCCCGCTCTCGTCTCCCGC TGCCACTTCAAGTCACGAAATGACAGTGTTATTCACATCCTTGTAGAGGTGACCACAGCGCAAGGTGCCG TTCACAGCTACCTGGGCTCCCCTTTTTGGATCCACCAGGCTGTGCTCCTTCCCACCCCGAGCCTGCACTG GAGGGAGGTCTCAAGTGGAAGGCTGGAGTTGGAGTGGCAGCACCAGTCATCTTGGGCAGCTCAAGAGACC TGCTACCAGCTCCGGTACACGGGAGAAGGCCGTGAGGACTGGAAGGAGACTGAGGCATGCTTTGTGGCCC TCGCTTCCAGACCTACACCGGGTCCTAGGCCAGTACCTCAGAGACACTGCAGCCCTAAGTCCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230049 representing NM_001285497 Red=Cloning site Green=Tags(s) MPSWALFMVTSCLLLALPNQAQVTSQDVFLLALGTEPLNCFSQTFEDLTCFWDEEEAAPSGTYQLLYAYR GEKPRACPLYSQSVPTFGTRYVCQFPAQDEVRLFFPLHLWVKNVSLNQTLIQRVLFVDSVGLPAPPRVIK ARGGSQPGELQIHWEAPAPEISDFLRHELRYGPTDSSNATAPSVIQLLSTETCCPTLWMPNPVPVLDQPP CVHPTASQPHGPVRTSPAGEAPFLTVKGGSCLVSGLQAGKSYWLQLRSQPDGVSLRGSWGPWSFPVTVDL PGDAVTIGLQCFTLDLKMVTCQWQQQDRTSSQGFFRHSRTRCCPTDRDPTWEKCEEEEPRPGSQPALVSR CHFKSRNDSVIHILVEVTTAQGAVHSYLGSPFWIHQAVLLPTPSLHWREVSSGRLELEWQHQSSWAAQET CYQLRYTGEGREDWKETEACFVALASRPTPGPRPVPQRHCSPKSF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001285497
ORF Size	1395 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001285497.1, NP_001272426.1
RefSeq Size	2701 bp
RefSeq ORF	1398 bp
Locus ID	17480
UniProt ID	Q08351
MW	52.4 kDa
Gene Summary	Receptor for thrombopoietin that acts as a primary regulator of megakaryopoiesis and platelet production. May represent a regulatory molecule specific for TPO-R-dependent immune responses.[UniProtKB/Swiss-Prot Function]

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