Npnt (NM_001287103) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1110009H02Rik; AA682063; AI314031; Nctn; POEM
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230002 representing NM_001287103 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACACTTTCGGCAGCTACAAGTGCTACTGTCTCAACGGATACATGCTGCTGCCAGACGGGTCCTGCT CAAGTGCCCTATCGTGTTCCATGGCAAACTGTCAGTATGGCTGTGACGTGGTCAAAGGACAGGTCCGATG TCAGTGTCCATCCCCTGGCCTGCAGCTAGCTCCCGATGGGAGGACCTGTGTGGATATTGATGAATGTGCG ACTGGAAGAGTCTCCTGCCCTCGATTTAGGCAGTGTGTCAACACGTTTGGGAGCTACATCTGCAAGTGTC ACACTGGTTTCGACCTCATGTACATTGGAGGCAAATATCAGTGCCATGACATCGACGAGTGCTCTCTTGG ACAGCACCAGTGTAGCAGCTATGCCCGGTGTTACAACATACATGGGTCCTACAAGTGCCAATGTAGAGAT GGATACGAGGGGGATGGACTGAACTGTGTGTATATCCCCAAAGTCATGATTGAACCTTCAGGTCCAATCC ATATGCCAGAAAGAAATGGTACAATCTCAAAGGGTGATGGAGGACATGCGAATAGGATTCCTGATGCTGG AAGTACAAGGTGGCCCCTGAAGACACCATATATTCCTCCTGTCATTACCAACAGGCCTACTTCCAAGCCA ACAACAAGACCTACACCAAACCCAACACCACAGCCTACTCCACCACCTCCACCACCCCTCCCGACAGAGC CCAGAACAACTCCACTACCACCAACCCCAGAAAGGCCATCTACCAGACCCACCACTATAGCACCTGCTAC CAGTACCACTACACGAGTAATTACGGTTGACAACAGGATACAGACGGATCCTCAGAAACCCAGAGGAGAT GTGTTCATTCCACGGCAGCCGACAAATGACCTGTTTGAGATATTTGAAATCGAAAGAGGGGTCAGCGCGG ATGAGGAAGTAAAGGACGACCCAGGTATTCTCATACACAGCTGCAATTTTGACCATGGACTCTGTGGATG GATCAGAGAAAAAGATAGTGACTTGCACTGGGAGACAGCCAGGGACCCAGCAGGTGGACAGTATCTCACA GTGTCTGCAGCCAAAGCCCCGGGGGGAAAAGCCGCTCGCTTGGTGTTACGTCTCGGCCACCTCATGCATT CAGGGGACCTGTGCCTGTCCTTTAGGCACAAGGTGACTGGGCTGCACTCGGGCACACTGCAGGTGTTTGT GAGGAAACACGGTACCCACGGAGCAGCCCTGTGGGGAAGAAATGGTGGCCATGGCTGGAGGCAAACCCAG ATCACCTTGCGAGGGGCTGACGTCAAAAGCGTCATCTTCAAAGGTGAAAAAAGGCGTGGTCACACGGGGG AGATTGGATTGGATGATGTGAGCTTGAAAAGAGGTCGCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230002 representing NM_001287103 Red=Cloning site Green=Tags(s) MNTFGSYKCYCLNGYMLLPDGSCSSALSCSMANCQYGCDVVKGQVRCQCPSPGLQLAPDGRTCVDIDECA TGRVSCPRFRQCVNTFGSYICKCHTGFDLMYIGGKYQCHDIDECSLGQHQCSSYARCYNIHGSYKCQCRD GYEGDGLNCVYIPKVMIEPSGPIHMPERNGTISKGDGGHANRIPDAGSTRWPLKTPYIPPVITNRPTSKP TTRPTPNPTPQPTPPPPPPLPTEPRTTPLPPTPERPSTRPTTIAPATSTTTRVITVDNRIQTDPQKPRGD VFIPRQPTNDLFEIFEIERGVSADEEVKDDPGILIHSCNFDHGLCGWIREKDSDLHWETARDPAGGQYLT VSAAKAPGGKAARLVLRLGHLMHSGDLCLSFRHKVTGLHSGTLQVFVRKHGTHGAALWGRNGGHGWRQTQ ITLRGADVKSVIFKGEKRRGHTGEIGLDDVSLKRGRC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001287103
ORF Size	1371 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001287103.1, NP_001274032.1
RefSeq Size	4337 bp
RefSeq ORF	1374 bp
Locus ID	114249
MW	50.5 kDa
Gene Summary	Functional ligand of integrin alpha-8/beta-1 in kidney development. Regulates the expression of GDNF with integrin alpha-8/beta-1 which is essential for kidney development. May also play a role in the development and function of various tissues, regulating cell adhesion, spreading and survival through the binding of several integrins.[UniProtKB/Swiss-Prot Function]

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