Sept7 (NM_001205367) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Cdc10; E430034N22
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229904 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGTCAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGCGGCACCATGGCTCAACCGA AGAACCTTGAGGGCTATGTGGGCTTTGCCAACCTCCCAAATCAAGTGTACAGAAAATCCGTGAAAAGAGG ATTTGAATTCACTCTTATGGTAGTAGGTGAATCTGGACTGGGAAAGTCGACATTAATCAACTCATTATTC CTCACAGATTTGTATTCTCCAGAGTATCCAGGACCTTCTCATAGAATCAAAAAGACTGTACAGGTGGAGC AATCCAAAGTTTTAATCAAAGAAGGTGGTGTTCAGTTGCTGCTGACAATAGTTGATACTCCAGGATTTGG AGATGCAGTGGATAATAGTAATTGCTGGCAGCCTGTTATCGACTACATTGATAGTAAATTTGAAGATTAC TTAAATGCAGAATCTCGAGTGAACAGACGTCAGATGCCTGATAACAGGGTGCAGTGTTGTTTATACTTCA TTGCTCCTTCAGGACATGGACTTAAACCATTGGACATTGAGTTTATGAAACGTTTGCATGAAAAAGTGAA TATCATCCCATTAATTGCCAAAGCAGACACACTTACACCAGAGGAATGCCAACAGTTTAAAAAGCAGATA ATGAAAGAAATTCAAGAACATAAAATTAAAATATATGAATTTCCAGAAACAGATGATGAAGAAGAGAATA AACTGGTTAAGAAGATAAAGGACCGTTTACCTCTTGCTGTGGTAGGTAGTAATACTATCATTGAAGTTAA TGGCAAAAGAGTCAGAGGAAGGCAGTATCCTTGGGGTGTGGCTGAAGTTGAAAATGGTGAACATTGTGAT TTCACAATTCTAAGAAATATGTTGATAAGAACACATATGCAGGACTTGAAAGATGTTACCAATAACGTAC ACTATGAGAACTACAGAAGCAGAAAGCTAGCAGCAGTGACTTACAATGGAGTGGATAACAACAAGAACAA AGGGCAGCTTACCAAGAGCCCTCTGGCACAGATGGAAGAAGAAAGAAGGGAACATGTAGCCAAAATGAAG AAGATGGAGATGGAGATGGAACAGGTGTTTGAGATGAAGGTCAAAGAAAAAGTTCAAAAACTGAAGGACT CTGAAGCAGAGCTCCAGCGGCGCCATGAGCAAATGAAAAAGAATTTAGAAGCACAGCACAAAGAATTAGA GGAAAAACGTCGTCAGTTTGAAGAAGAAAAGGCAAACTGGGAAGCTCAACAGCGTATTTTAGAGCAACAG AACTCTTCAAGAACCTTGGAAAAGAACAAGAAGAAAGGCAAGATCTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229904 protein sequence Red=Cloning site Green=Tags(s) MSVSARSAAAEERSVNCGTMAQPKNLEGYVGFANLPNQVYRKSVKRGFEFTLMVVGESGLGKSTLINSLF LTDLYSPEYPGPSHRIKKTVQVEQSKVLIKEGGVQLLLTIVDTPGFGDAVDNSNCWQPVIDYIDSKFEDY LNAESRVNRRQMPDNRVQCCLYFIAPSGHGLKPLDIEFMKRLHEKVNIIPLIAKADTLTPEECQQFKKQI MKEIQEHKIKIYEFPETDDEEENKLVKKIKDRLPLAVVGSNTIIEVNGKRVRGRQYPWGVAEVENGEHCD FTILRNMLIRTHMQDLKDVTNNVHYENYRSRKLAAVTYNGVDNNKNKGQLTKSPLAQMEEERREHVAKMK KMEMEMEQVFEMKVKEKVQKLKDSEAELQRRHEQMKKNLEAQHKELEEKRRQFEEEKANWEAQQRILEQQ NSSRTLEKNKKKGKIF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001205367
ORF Size	1308 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001205367.1, NP_001192296.1
RefSeq Size	2530 bp
RefSeq ORF	1311 bp
Locus ID	235072
UniProt ID	O55131
MW	50.5 kDa
Gene Summary	Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.