Spata7 (NM_001289573) Mouse Tagged ORF Clone
CAT#: MR229790
- TrueORF®
Spata7 (myc-DDK-tagged) - Mouse spermatogenesis associated 7 (Spata7), transcript variant 3
"NM_001289573" in other vectors (1)
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CNY 4,180.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI661438; B230306G18Rik; HSD3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR229790 representing NM_001289573
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGGCAGCCGGAGAGTCAGAGCAACCTCTGTCCTCCCCAGATACAGCCCTCCATGCTTGTTTACAG GACACCTGAGCACCAAGAGCAACGCTTTTTGCACTGATTCCTCTTCTCTCAGACTAAGCACCCTGCAGCT GGTCAAGAACCACATGGCTATTCACTATAATAAAATCCTTTCAGCCAAAGCCGCAGTGGACTGCTCAATT CCAGTAAGTGTGAATACTAGCATCAAATATGCTGACCAACAACGAAGAGAAAAACTGAGGAAGGAGTTGG CACGGTGTGAAAAGGAGTTTAAATTAAGTAAATCTGCTATGCAGACCAATTCTAAGATGAATTCCAAGTT CTTTGTAAATTCTCTACAGAAGGTACCTAATTACACGAGAAATGGTGCTGAAGACAAAATAGCTCCTTTA CCCTCACAAGGACAAAACTTAGCATGGGACAGTATTCAAGATGGGATTCTGCAGCAGTCCTCAGAAAGGG CATCCTGTAAGCTCTCCACAGAGTTTTCTCCAGACAGTAAAATCTACTCTGATGAAGAAGAGCTGCTGTA CCTGAGTTTCATGGAAAATGTGACGGATGAAATCTTGAAACTTGGTTTATTTTCTAACAGGTTTCTAGAG CGACTGTTTGAGCGACATATAAAGAAAAATAAACATCATTTGGAGGAGGGAAAGATGCGCTACCTGCTGC ATGGGCTGAAGGTTGACTTAGGCTGCATATCTGAGGAAGACCCAGCAAAGCAAAAACATTTCAGAATGTT GAATCAACTTCATTTTCAAAAGGCTCTGATTTCAAGAGAAAATGAGTTTGTAAGTGATGAAGAAACAGTG AGTCACCACGAGCGCCAGCAGTACCAGGAGGCCCTGGACATGCTGTCGGCTGTGCCGAAGGACGAAAACA AGATGTTCTCTTTACCGGGCGAATTTTTAATACCCGCCCATAAAGTCAAGCATTCAGAGGGTGTTATAAT TCAACAGGTCAATGACGAAACAGATAATGAAGCCTCACCCTGGAATGAAAACAATCCAAGTGTTTCTGAT AGTGTAATAGACCAGGAAACCTCTGTGGATGTCATTGAAGGTGACAGTGACTTTGAAAGGGCTGAGACTT CCAGGGAACTCTGTTGTCTGAGCACATCACTGTCCCCATCTGGTCCATTCCCCAGCATCAATGGTGGCAG TAATCATGGTAAGGAATTATCGACTCTACGAATCATGGGAATGAGCATTGAAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR229790 representing NM_001289573
Red=Cloning site Green=Tags(s) MDGSRRVRATSVLPRYSPPCLFTGHLSTKSNAFCTDSSSLRLSTLQLVKNHMAIHYNKILSAKAAVDCSI PVSVNTSIKYADQQRREKLRKELARCEKEFKLSKSAMQTNSKMNSKFFVNSLQKVPNYTRNGAEDKIAPL PSQGQNLAWDSIQDGILQQSSERASCKLSTEFSPDSKIYSDEEELLYLSFMENVTDEILKLGLFSNRFLE RLFERHIKKNKHHLEEGKMRYLLHGLKVDLGCISEEDPAKQKHFRMLNQLHFQKALISRENEFVSDEETV SHHERQQYQEALDMLSAVPKDENKMFSLPGEFLIPAHKVKHSEGVIIQQVNDETDNEASPWNENNPSVSD SVIDQETSVDVIEGDSDFERAETSRELCCLSTSLSPSGPFPSINGGSNHGKELSTLRIMGMSIED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001289573 |
ORF Size | 1245 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001289573.1, NP_001276502.1 |
RefSeq Size | 1570 bp |
RefSeq ORF | 1248 bp |
Locus ID | 104871 |
MW | 47.4 kDa |
Gene Summary | Involved in the maintenance of both rod and cone photoreceptor cells (PubMed:25398945, PubMed:29100828, PubMed:29899041). Required for photoreceptor-specific localization of proximal connecting cilium (CC) proteins RPGR, AHI1, NPHP1, NPHP4, and RPGRIP1 at the distal CC, a photoreceptor-specific extension of the primary cilium transition zone (PubMed:25398945, PubMed:29100828, PubMed:29899041). Maintenance of protein localization at the photoreceptor-specific distal CC is essential for normal microtubule stability and to prevent photoreceptor degeneration (PubMed:25398945, PubMed:29899041).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC227579 | Spata7 (untagged) - Mouse spermatogenesis associated 7 (Spata7), transcript variant 3 |
CNY 3,900.00 |