Spn (NM_009259) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	A630014B01Rik; Cd43; Galgp; Ly-48; Ly48
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229682 representing NM_009259 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTTGCACCTTCTCCTCCTCTTTGGGGCATGCTGGGTCCAGGTGGCGAGCCCAGACAGTCTGCAGA GGACGACGATGCTACCATCTACCCCACATATCACAGCTCCAAGTACCTCTGAAGCCCAGAATGCCAGCCC ATCAGTTTCTGTGGGGTCAGGGACAGTGGACTCGAAGGAGACCATCAGCCCCTGGGGGCAGACCACAATC CCAGTTTCTTTGACCCCCTTGGAAACTACTGAATTGTCTTCTTTGGAGACTTCTGCTGGTGCCAGCATGA GCACCCCTGTACCTGAGCCTACTGCCTCTCAGGAAGTTTCCAGCAAGACATCAGCACTGCTTCCAGAACC ATCAAATGTAGCCAGTGACCCTCCTGTCACTGCAGCCAATCCTGTGACAGACGGACCTGCAGCCAATCCT GTGACAGACGGAACTGCAGCATCTACATCTATCTCTAAAGGAACCAGTGCACCCCCCACCACTGTGACAA CAAGCTCTAACGAGACCAGTGGACCCTCTGTGGCTACAACAGTAAGCTCCAAGACCAGTGGTCCCCCTGT CACCACAGCTACTGGGTCTCTGGGGCCCTCGAGTGAGATGCATGGACTCCCTGCTACCACAGCAACCAGT TCTGTGGAGAGTTCCAGTGTGGCCCGTGGCACCTCAGTTTCCAGCAGAAAAACATCCACGACGTCTACCC AAGATCCCATAACCACCAGGTCACCAAGCCAAGAATCAAGTGGCATGTTACTGGTGCCCATGCTTATTGC CTTGGTGGTGGTTTTGGCCCTCGTGGCGCTACTGCTGTTGTGGCGCCAGAGGCAAAAGCGGAGGACTGGG GCCCTGACCCTGAGCGGAGGAGGAAAACGAAATGGGGTGGTGGATGCCTGGGCTGGGCCAGCCCGGGTTC CTGATGAAGAGGCCACAACCACATCAGGGGCAGGGGGCAACAAGGGCTCTGAGGTACTGGAGACAGAAGG CTCCGGGCAGAGACCCACGCTCACTACGTTCTTCAGCAGACGGAAGTCTCGCCAGGGCTCTTTAGTACTA GAAGAGCTGAAGCCTGGGTCGGGTCCCAACCTGAAGGGGGAGGAAGAGCCGCTTGTGGGCAGTGAGGATG AAGCTGTGGAAACCCCAACTTCTGACGGTCCACAAGCCAAAGATGAGGCCGCACCTCAATCTCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229682 representing NM_009259 Red=Cloning site Green=Tags(s) MALHLLLLFGACWVQVASPDSLQRTTMLPSTPHITAPSTSEAQNASPSVSVGSGTVDSKETISPWGQTTI PVSLTPLETTELSSLETSAGASMSTPVPEPTASQEVSSKTSALLPEPSNVASDPPVTAANPVTDGPAANP VTDGTAASTSISKGTSAPPTTVTTSSNETSGPSVATTVSSKTSGPPVTTATGSLGPSSEMHGLPATTATS SVESSSVARGTSVSSRKTSTTSTQDPITTRSPSQESSGMLLVPMLIALVVVLALVALLLLWRQRQKRRTG ALTLSGGGKRNGVVDAWAGPARVPDEEATTTSGAGGNKGSEVLETEGSGQRPTLTTFFSRRKSRQGSLVL EELKPGSGPNLKGEEEPLVGSEDEAVETPTSDGPQAKDEAAPQSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_009259
ORF Size	1185 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_009259.5, NP_033285.1
RefSeq Size	3751 bp
RefSeq ORF	1188 bp
Locus ID	20737
UniProt ID	P15702
MW	40 kDa
Gene Summary	Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (PubMed:17638845, PubMed:21289089, PubMed:11728336). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment (PubMed:18490738). Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells. Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (By similarity). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin (PubMed:26700769). Acts as a T-cell counter-receptor for SIGLEC1 (PubMed:11238599).[UniProtKB/Swiss-Prot Function]

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