Crtam (NM_001281954) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229676 representing NM_001281954 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGTGGGGAGCCCTCAGTTTGCTGTTCTGGGTGCCGGTGCAAGCAGCCTTTCTGAAAATGGAGACCG TCACGGTAGAGGAAGGCCAAACGCTCACTCTAACGTGTGTTACTTCTCAGACAAAAAACGTCTCTCTCCA GTGGCTGGCTCCCTCTGGGTTCACCATTTTTTTAAACCAGCATCCTGCTTTAAAAAGCTCCAAATACCAG CTTCTTCACCATTCAGCTACACAACTCTCCATTAGTGTGTCCAATGTAACTCTCCGAGAGGAAGGTGTGT ATACATGCTTGCACTACGGGAGTTCTGTGAAGACGAAGCAAGTGAGAGTGACCGTGTTAGTGACTCCTTT CCAGCCAACCGTGGAAGCTTTGGTTCTCAGAAGGCAGAATGGAGAGAAATCGGTTGTGCTGAAATGTTCC ACAGAGAGAAGCAAGCCCCCGCCACAAATCACCTGGCTGCTGGGGGAAGGTCTGGAGATCTATGGTGAAC TCAACCATGAATTTGAAGCTGATGGGAAAATATGTAACACCAGCAGCATGCTCATCGCCCGCGCATACGG CAAAAACTCAACCGTGCACTGCATTATCCAGCACGAGGGCTTGCACGGGAGAAAGCTGGTTGCCCCCTTC CAGTTTGAAGATTTGGTTGCAGATCAAGAAACTTCAGATCAAGAAACTTCAGATGCCCCTGAGCAGAGCT CTCTCTCCTCCCAAGCCCTCCAGCAGCCCACAAGTACAGTCTCAATGATGGAAAATTCCAGTATACCAGA GACTGACAAGGAAGAGAAAGAACATGCCACTCAAGACCCTGGCTTGTCCACTGAAGCCAGTGCTCAGCAT ACAGGACTGGCCCGGAGGAAGAGTGGTATCCTGCTGCTCACACTGGTGTCCTTCCTCATTTTCATCCTTT TCATCATCGTTCAGCTCTTCATCATGAAGCTGCGTAAAGCACACGTGGTATGGAAGAAGGAAAGTGAAAT TTCAGAGCAAGCTCTAGAAAGTTACAGATCAAGATCCAACAACGAGGAGACATCTTCCCAGGAGAATAGC AGCCAGGCTCCCCAGTCTAAGCGTTGCATGAACTACATCACGCGGTTATACTCGGGAGCCAAAACAAAGA AGAGTGCCCAGCACTGGAAGCTCGGAGGCAAGCACAGCCGTGTCCCGGAGAGTATTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229676 representing NM_001281954 Red=Cloning site Green=Tags(s) MWWGALSLLFWVPVQAAFLKMETVTVEEGQTLTLTCVTSQTKNVSLQWLAPSGFTIFLNQHPALKSSKYQ LLHHSATQLSISVSNVTLREEGVYTCLHYGSSVKTKQVRVTVLVTPFQPTVEALVLRRQNGEKSVVLKCS TERSKPPPQITWLLGEGLEIYGELNHEFEADGKICNTSSMLIARAYGKNSTVHCIIQHEGLHGRKLVAPF QFEDLVADQETSDQETSDAPEQSSLSSQALQQPTSTVSMMENSSIPETDKEEKEHATQDPGLSTEASAQH TGLARRKSGILLLTLVSFLIFILFIIVQLFIMKLRKAHVVWKKESEISEQALESYRSRSNNEETSSQENS SQAPQSKRCMNYITRLYSGAKTKKSAQHWKLGGKHSRVPESIV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001281954
ORF Size	1179 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001281954.1, NP_001268883.1
RefSeq Size	1939 bp
RefSeq ORF	1182 bp
Locus ID	54698
UniProt ID	Q149L7
MW	44.4 kDa
Gene Summary	Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo.[UniProtKB/Swiss-Prot Function]

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