Asmt (NM_001199212) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Hio; Hiomt
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229648 representing NM_001199212 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACAGGGGCCGCTCGGCCTCCGCCCGCCAGGAGCGCGACTTCCGGGCCCTCATGGACCTGGCCCACG GCTTCATGGCCTCCCAGGTGCTGTTCGCGGGCTGCGCGCTCCGCGTGTTCGACGCCGCGGCCCTGGGCCC CGTGGACGCCGCGGCGCTGGCGAGGTCGTCGGGCCTGAGCCCCCGGGGGACGCGGCTGCTGCTCGACGCC TGCGCGGGGCTGGGGCTGCTGCGGAGACGCAGGGGGGCGGGGCCTCGCGGCCCCGCCTACACCAACTCCC CCCTGGCGTCCACCTTCCTGGTCGCGGGCAGCCCCCTGTCTCAGCGCAGCCTCCTGCTCTACCTGGCGGG CACCACCTACCTGTGCTGGGGGCACCTGGCGGACGGCGTGAGGGAAGGGCGGAGCCAGTACGCGAGGGCC GTGGGCGTCGACGCGGACGACCCCTTCACCGCCATCTACAGGTCGGAGGCCGAGCGCCTGCTGTTCATGC GGGGCCTGCAGGAGACCTGGAGCCTGTGCGGGGGGCGGGTCCTCACGGCCTTCGACCTCTCGCCCTTCAG GGTCATCTGCGACCTCGGTGGTGGGTCCGGGGCGCTGGCCCGCATGGCCGCCCGGCTCTACCCGGGCAGC GAGGTCACCGTGTTCGAGACGCCCGACGTCGTCGCCGCCGCCCGCGCCCACTTCCCGCCCCCAGCGGACG AGGACGGGGCGGAGCCTCGTGTGCGCTTCCTGTCAGGCGACTTCTTCCGCTCGCCGCTGCCGCCCGCCGA CCTCTACGTCCTGGCCCGGGTCCTGCACGACTGGGCGGACGCCGCCTGCGTGGAGCTGCTGCGGCGCGTG CGGGGCGCCCTGCGGCCAGGCGGCGCGGTGCTGCTGGTGGAGAGCGTGCTGTCCCCGGGAGGGGCGGGGC CGACGCGGACGCTGCTGCTGTCGCTCACGATGCTGCTGCAGGCCCGGGGCCGCGAGCGCACGGAGGCCGA GTACCGGGCGCTGACCGCGCGCGCCGGCTTCTCCCGCCTGCGGCTGCGGCGCCCGCGGGGCCCCTACCAC GCCATGATGGCCGCCCGGGGGGGCGGGGCCGGGGCGCGGAGCGACGGCGGCGGCGGGGACGCGACGTCAC AGACAGGAAGTGGGACAGGAAGTGAGGTCGGCGCCCAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229648 representing NM_001199212 Red=Cloning site Green=Tags(s) MHRGRSASARQERDFRALMDLAHGFMASQVLFAGCALRVFDAAALGPVDAAALARSSGLSPRGTRLLLDA CAGLGLLRRRRGAGPRGPAYTNSPLASTFLVAGSPLSQRSLLLYLAGTTYLCWGHLADGVREGRSQYARA VGVDADDPFTAIYRSEAERLLFMRGLQETWSLCGGRVLTAFDLSPFRVICDLGGGSGALARMAARLYPGS EVTVFETPDVVAAARAHFPPPADEDGAEPRVRFLSGDFFRSPLPPADLYVLARVLHDWADAACVELLRRV RGALRPGGAVLLVESVLSPGGAGPTRTLLLSLTMLLQARGRERTEAEYRALTARAGFSRLRLRRPRGPYH AMMAARGGGAGARSDGGGGDATSQTGSGTGSEVGAQD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199212
ORF Size	1161 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199212.1, NP_001186141.1
RefSeq Size	1273 bp
RefSeq ORF	1164 bp
Locus ID	107626
UniProt ID	D3KU66
MW	41.4 kDa
Gene Summary	This gene belongs to the methyltransferase superfamily and is located in the pseudoautosomal region (PAR) of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin and is abundant in the pineal gland. Two amino acid substitutions (R78G and R242C) are present in the encoded protein derived from the reference strain, C57BL/6J, and this protein shows low enzyme activity relative to the protein derived from other strains. [provided by RefSeq, May 2015]

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