Bscl2 (NM_001290823) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2900097C17Rik; AI046355; Gng3lg
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229624 representing NM_001290823 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCAATGACCCACCAGTCCCCGCCTTACTGTGGGCCCAGGAAGTGGGCCACGTCTTGGCAGGCCGTG CCCGCAGGCTGATGCTGCAGTTTGGGGTGCTCTTCTGCACCATCCTTCTTTTGCTTTGGGTGTCTGTGTT CCTCTATGGCTCCTTCTACTACTCCTACATGCCGACGGTCAGCCACCTCAGCCCTGTGCACTTCCACTAC AGGACAGACTGTGATTCCTCCACCGCCTCTCTCTGCTCTTTCCCTGTTGCCAATGTCTCACTGGCTAAGA GTGGACGTGATCGGGTACTGATGTATGGACAGCCATATCGAGTCACCTTAGAGCTTGAACTGCCCGAATC CCCTGTGAATCAAGACTTGGGCATGTTCTTAGTCACTGTTTCATGTTATACCAGAGGTGGCCGAATCATC TCCACTTCTTCACGCTCAGTGATGCTGCATTACCGCTCGCAGCTGCTCCAGGTGCTCGACACGCTGCTGT TCTCCAGCCTCCTGCTGTTTGGCTTCGCTGAACAGAAGCAGCTCCTGGAAGTAGAACTCTACTCTGACTA TAGGGAGAATTCGTATGTGCCAACAACTGGAGCAATTATTGAGATCCACAGCAAGCGCATCCAGATGTAT GGAGCCTACCTCCGGATCCATGCCCACTTCACTGGGCTCAGGTACCTGCTGTACAACTTCCCCATGACCT GCGCCTTCGTGGGTGTGGCCAGCAACTTCACGTTCCTCAGCGTCATCGTGCTCTTCAGCTATATGCAGTG GGTGTGGGGTGCTGTCTGGCCCCGCCACCGCTTCTCTCTGCAGGTTAATATCCGACAAAGGGATAACTCA CACCACGGGGCCCCGCGTCGGATCTCGCGCCATCAGCCAGGTCAGGAATCTACCCAGCAGTCGGATGTGA CGGAGGATGGTGAGAGCCCTGAAGATCCCTCAGGAACAGAGGGCCAGCTGTCTGAGGAAGAGAAACCAGA GAAGCGGCCCCTGAATGGAGAGGAGGAGCAAGAGCCAGAGGCTAGTGATGGCTCCTGGGAAGATGCAGCT TTGCTGACAGAGGCCAACCCACCTACCTCTGCCTCAGCATCTGCCCTTGCCCCTGAGACTCTGGGTAGCC TCAGGCAACGCCCGACCTGCTCCAGTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229624 representing NM_001290823 Red=Cloning site Green=Tags(s) MVNDPPVPALLWAQEVGHVLAGRARRLMLQFGVLFCTILLLLWVSVFLYGSFYYSYMPTVSHLSPVHFHY RTDCDSSTASLCSFPVANVSLAKSGRDRVLMYGQPYRVTLELELPESPVNQDLGMFLVTVSCYTRGGRII STSSRSVMLHYRSQLLQVLDTLLFSSLLLFGFAEQKQLLEVELYSDYRENSYVPTTGAIIEIHSKRIQMY GAYLRIHAHFTGLRYLLYNFPMTCAFVGVASNFTFLSVIVLFSYMQWVWGAVWPRHRFSLQVNIRQRDNS HHGAPRRISRHQPGQESTQQSDVTEDGESPEDPSGTEGQLSEEEKPEKRPLNGEEEQEPEASDGSWEDAA LLTEANPPTSASASALAPETLGSLRQRPTCSSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290823
ORF Size	1149 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290823.1, NP_001277752.1
RefSeq Size	1421 bp
RefSeq ORF	1152 bp
Locus ID	14705
UniProt ID	Q9Z2E9
MW	43.6 kDa
Gene Summary	Is a regulator of lipid catabolism essential for adipocyte differentiation. Necessary for correct lipid storage and lipid droplets maintenance; may play a tissue-autonomous role in controlling lipid storage in adipocytes and in preventing ectopic lipid droplet formation in non-adipose tissues. May also be involved in the central regulation of energy homeostasis.[UniProtKB/Swiss-Prot Function]

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