Dlk2 (NM_001286028) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI413481; DLK-2; Egfl9
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229623 representing NM_001286028 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAGCGGCTGCCGCTGCCTAAATCTCGTGTGCCTGCTGTGCATCCTGGGGGCAACCAGCCAGCCTG CCAGAGCGGATGACTGCAGCTCCCACTGTGACCTGGCCCACGGCTGCTGCGCTCCTGACGGCTCCTGCAG GTGTGACCCAGGCTGGGAGGGGCTGCACTGTGAGCGCTGTGTGAGGATGCCTGGCTGCCAGCACGGTACC TGCCACCAGCCCTGGCAGTGCATCTGCCACAGTGGCTGGGCAGGAAAGTTCTGTGACAAAGATGAGCACA TCTGTACCTCACAATCACCCTGCCAGAATGGAGGCCAGTGTGTGTATGACGGGGGTGGTGAGTACCACTG TGTGTGCCTGCCAGGCTTCCATGGACGTGGCTGTGAGCGCAAGGCTGGACCTTGTGAGCAGGCAGGCTTC CCGTGCCGGAATGGAGGGCAGTGCCAGGACAACCAGGGTTTTGCCCTCAACTTCACATGCCGCTGCTTGG CAGGATTCATGGGTGCCCACTGTGAGGTCAATGTGGACGACTGTCTGATGCGGCCTTGCGCCAATGGTGC CACATGCATTGATGGCATAAACCGCTTTTCCTGCCTCTGTCCTGAGGGCTTCGCTGGACGCTTCTGCACC ATCAACCTGGATGACTGTGCCAGCCGCCCATGCCAGAGAGGGGCCCGCTGCAGGGACCGTGTCCATGACT TTGACTGCCTGTGCCCCAGTGGCTATGGTGGTAAGACTTGTGAACTTGTCCTACCTGCTCCAGAGCCTGC CTCAGTGGGCACCCCACAAATGCCCACCTCAGCTGTAGTTGTACCTGCCACGGGGCCGGCCCCTCACAGT GCGGGGGCGGGCCTGCTAAGGATCTCAGTGAAGGAGGTGGTACGGAGGCAAGAGTCTGGGCTTGGTGAAT CTAGCCTGGTTGCCCTGGTGGTGTTTGGGTCTCTCACTGCTGCGCTGGTCCTGGCCACGGTGCTGCTGAC CCTGAGGGCATGGCGCCGAGGTATATGCCCCACTGGACCCTGCTGCTACCCAGCCCCACACTATGCCCCA GCTCGGCAGGATCAGGAGTGCCAGGTTAGCATGCTGCCAGCGGGGTTCCCTCTGTCCCCAGACCTGCCCC CTGAGCCTGGTAAGACCACAGCGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229623 representing NM_001286028 Red=Cloning site Green=Tags(s) MPSGCRCLNLVCLLCILGATSQPARADDCSSHCDLAHGCCAPDGSCRCDPGWEGLHCERCVRMPGCQHGT CHQPWQCICHSGWAGKFCDKDEHICTSQSPCQNGGQCVYDGGGEYHCVCLPGFHGRGCERKAGPCEQAGF PCRNGGQCQDNQGFALNFTCRCLAGFMGAHCEVNVDDCLMRPCANGATCIDGINRFSCLCPEGFAGRFCT INLDDCASRPCQRGARCRDRVHDFDCLCPSGYGGKTCELVLPAPEPASVGTPQMPTSAVVVPATGPAPHS AGAGLLRISVKEVVRRQESGLGESSLVALVVFGSLTAALVLATVLLTLRAWRRGICPTGPCCYPAPHYAP ARQDQECQVSMLPAGFPLSPDLPPEPGKTTAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286028
ORF Size	1146 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286028.1, NP_001272957.1
RefSeq Size	1415 bp
RefSeq ORF	1149 bp
Locus ID	106565
UniProt ID	Q8K1E3
MW	40.9 kDa
Gene Summary	Regulates adipogenesis.[UniProtKB/Swiss-Prot Function]

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