Btbd8 (NM_001255991) Mouse Tagged ORF Clone
CAT#: MR229579
- TrueORF®
Btbd8 (myc-DDK-tagged) - Mouse BTB (POZ) domain containing 8 (Btbd8)
"NM_001255991" in other vectors (1)
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CNY 3,800.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | EG627196; Gm16115; OTTMUSG00000030610 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR229579 representing NM_001255991
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCACTGTGGAGCGGGAGGTGCGGCCCCGGTGGCCCTTCTGAGTTCCCCGGGGCATGGCAGGAAGG GGCTGCCCAGGAAGGGGCCCGGGGAGCGGCGCCGGCTGAAGGCGGCGGTGTCGGAGCAGCTCAGCCGCGA CGTGCTCAGGCTTTTAAGGGAGGAAATCCACACAGACACCATTCTTTCTGTCAGTGGCTCGCTGTTCAAA GTGCACAAGGCAGTCTTGTTAGCCAGAGCTCCTGGCTTTCATTCCCATATCATTGAACACACATCAAGTG ACCTAACAAATGAGCTTGTTCCTGTGGATGGTGTTGAAGCTTCAGAATTTAAAGCATTTTTACAGATTGT ATATTCATCAAACAAAAACATAAAAAACTATGAAGAGGAAATTGTTAAAAAACTGAAAGTGGGAAGCTTA ATGCCAGAAAAGGGGCCAGACGTCAGCTTCCCGCGGTATAGAACTTCGTCTGACTGTTTTCTTGGGAAAG GTGAAATTCCAGAGGATATTACTGGTGGAGGTGACTGCTTTATTTCCAAGGCTGATTCTGACCTGGAGCC TGCGTCTGAGTTAGGAGGAGATTTGTTGAAGCTTTACCTGAGTCAGCACTACCCTGACATTGACATTTGT GTTGATGGGAGGAGCTTCCGAGCCCACAGGGCCATTTTAAGTGCCAGGTCGAGTTATTTTGCTGCAATGC TGAGTGGCTGCTGGGCTGAAAGCTCCCAAGAGTGTATTACTCTTCAAGGAATAACCCATGTAGAAATGAA TGTTATGATGCACTTTATATATGGAGGAACTTTGGATTTTCCCGAAAAGGCTAATGTTGGTCAGATACTG AACGTGGCTGATATGTATGGACTGGAAGGATTGAGAGAAGTAGCAATCTATGTTTTAAGAAGAGATTACT GTAATTTCTTTCAGAAGCCTGTTCCCAGAACGTGGGCATCAATTCTAGAATGCTTAATCATTGCTCATTC AGTCGGAGTGGAAAGCCTTTTTGCTGACTGCATGAACTGCATTATAAGCCATTTTGCAAGGTTTTGGTCG GAAAGAAGCTTTGCAAATGTTCCTCCTGAGATTCAGAAAACCTGTCTCAGTATGCAGATTCAGTCCCTAG TGAGTATAGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR229579 representing NM_001255991
Red=Cloning site Green=Tags(s) MAHCGAGGAAPVALLSSPGHGRKGLPRKGPGERRRLKAAVSEQLSRDVLRLLREEIHTDTILSVSGSLFK VHKAVLLARAPGFHSHIIEHTSSDLTNELVPVDGVEASEFKAFLQIVYSSNKNIKNYEEEIVKKLKVGSL MPEKGPDVSFPRYRTSSDCFLGKGEIPEDITGGGDCFISKADSDLEPASELGGDLLKLYLSQHYPDIDIC VDGRSFRAHRAILSARSSYFAAMLSGCWAESSQECITLQGITHVEMNVMMHFIYGGTLDFPEKANVGQIL NVADMYGLEGLREVAIYVLRRDYCNFFQKPVPRTWASILECLIIAHSVGVESLFADCMNCIISHFARFWS ERSFANVPPEIQKTCLSMQIQSLVSIV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001255991 |
ORF Size | 1131 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001255991.1, NP_001242920.1 |
RefSeq Size | 1134 bp |
RefSeq ORF | 1134 bp |
Locus ID | 100503185 |
UniProt ID | D3YUB6 |
MW | 42 kDa |
Gene Summary | Involved in clathrin-mediated endocytosis at the synapse. Plays a role in neuronal development and in synaptic vesicle recycling in mature neurons, a process required for normal synaptic transmission.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC227368 | Btbd8 (untagged) - Mouse BTB (POZ) domain containing 8 (Btbd8) |
CNY 3,610.00 |