Azin1 (NM_001301688) Mouse Tagged ORF Clone
CAT#: MR229499
- TrueORF®
Azin1 (myc-DDK-tagged) - Mouse antizyme inhibitor 1 (Azin1), transcript variant 3
"NM_001301688" in other vectors (1)
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CNY 3,610.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1700085L02Rik; AZI; O; Oazi; Oazin |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR229499 representing NM_001301688
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGGATTTATTGACGATGCGAACTACTCCGTTGGCCTGTTGGATGAAGGAACAAACCTTGGAAATG TTATTGATAACTATGTTTATGAACATACCCTGACAGGAAAAAATGCATTTTTTGTGGGGGATCTTGGGAA GATCGTGAAGAAGCACAGTCAGTGGCAGACCGTGGTGGCTCAGATAAAGCCGTTTTACACGGTGAAGTGC AACTCCACTCCAGCCGTGCTTGAGATCTTGGCAGCTCTTGGAACTGGGTTTGCTTGTTCCAGCAAAAATG AAATGGCTTTAGTGCAAGAATTGGGTGTATCTCCAGAAAACATCATTTTCACAAGTCCTTGTAAGCAAGT GTCTCAGATAAAGTATGCAGCAAAAGTTGGAGTAAATATTATGACATGTGACAATGAGATTGAATTAAAG AAAATTGCAAGGAATCACCCAAATGCCAAGGTCTTACTACATATTGCAACAGAAGATAATATTGGAGGTG AAGATGGTAACATGAAGTTTGGCACTACACTGAAGAATTGTAGGCATCTTTTGGAATGTGCCAAGGAACT TGATGTCCAAATAATTGGGGTTAAATTTCATGTTTCAAGTGCTTGCAAAGAATATCAAGTATATGTACAT GCCCTGTCTGATGCTCGATGTGTGTTTGACATGGCTGGAGAGTTTGGCTTTACAATGAACATGTTAGACA TCGGTGGAGGCTTCACAGGAACTGAAATTCAGTTGGAAGAGGTTAATCATGTTATCAGTCCTCTGTTGGA TATTTACTTCCCTGAAGGATCTGGCATTCAGATAATTTCAGAACCTGGAAGCTACTATGTATCTTCTGCG TTTACACTTGCAGTCAATATTATTGCTAAGAAAGTTGTTGAAAATGATAAATTTTCCTCTGGAGTAGAAA AAAATGGGAGTGATGAGCCAGCCTTCGTGTATTACATGAATGATGGTGTTTATGGTTCTTTTGCGAGTAA GCTTTCTGAGGACTTAAATACCATTCCAGAGGTTCACAAGAAATACAAGGAAGATGAGCCTCTGTTTACA AGCAGCCTTTGGGGTCCATCCTGTGATGAGCTTTTCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR229499 representing NM_001301688
Red=Cloning site Green=Tags(s) MKGFIDDANYSVGLLDEGTNLGNVIDNYVYEHTLTGKNAFFVGDLGKIVKKHSQWQTVVAQIKPFYTVKC NSTPAVLEILAALGTGFACSSKNEMALVQELGVSPENIIFTSPCKQVSQIKYAAKVGVNIMTCDNEIELK KIARNHPNAKVLLHIATEDNIGGEDGNMKFGTTLKNCRHLLECAKELDVQIIGVKFHVSSACKEYQVYVH ALSDARCVFDMAGEFGFTMNMLDIGGGFTGTEIQLEEVNHVISPLLDIYFPEGSGIQIISEPGSYYVSSA FTLAVNIIAKKVVENDKFSSGVEKNGSDEPAFVYYMNDGVYGSFASKLSEDLNTIPEVHKKYKEDEPLFT SSLWGPSCDELFH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001301688 |
ORF Size | 1089 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001301688.1, NP_001288617.1 |
RefSeq Size | 4620 bp |
RefSeq ORF | 1092 bp |
Locus ID | 54375 |
UniProt ID | O35484 |
MW | 40.3 kDa |
Gene Summary | The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 1, the first member of this gene family that is ubiquitously expressed, and is localized in the nucleus and cytoplasm. Overexpression of antizyme inhibitor 1 gene has been associated with increased proliferation, cellular transformation and tumorigenesis. Gene knockout studies showed that homozygous mutant mice lacking functional antizyme inhibitor 1 gene died at birth with abnormal liver morphology. RNA editing of this gene, predominantly in the liver tissue, has been linked to the progression of hepatocellular carcinoma. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2014] |
Documents
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC227288 | Azin1 (untagged) - Mouse antizyme inhibitor 1 (Azin1), transcript variant 3 |
CNY 3,520.00 |