Phf23 (NM_001291125) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	JUNE-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229306 representing NM_001291125 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGAAGCCATGGCGGAGCCCAGTCCCGAAGATCCGCCTCCGACTCTTAAACCAGAGACTCAGCCAC CGGAGAAAAGGCGGAGAACCATTGAGGATTTTAACAAATTCTGCAGTTTTGTCTTGGCCTATGCTGGTTA CATCCCCCCTAGCAAAGAGGCTCCTGACAGTGCTACTCTGCTTGAGAAGATGAAGCTCAAGGACTCTCTT TTTGATCTGGATGGGCCCAAAGTGGCATCTCCACTCTCTCCCACATCATTGACGCATACTTCCCGGCCCC CCGCTGCTCTCGCCCCAGTGCCGCTGTCCCAGGGGGACCTCTCCCAGCCTCGAAAGAAGGACCGAAAGAA TAGAAAGTTGGGACCAGGAGGTGGGGCTGGCTTTGGGGTGCTTCGGAGACCTCGACCAGCTCCTGGGGAT GGGGAAAAGCGGTCTCGAATCAAGAAGAGCAAGAAGCGGAAGTTGAAAAAGGCAGATCGGGGAGATAGAC TCCCACCTCCTGGCCCTCCTAGGGCTCCTCCCAGTGATACAGACTCTGAAGAGGAGGAGGAAGAGGAAGA AGAGGAAGATGATGAGGAAGAGATGACAGTGGGGGGTGGAGTCCCAGCCCCTGTGCTCCCAACACCCCCT GAGGCCCCCAGGCCCCCTGTTACAGTGCACTCTGAAGGTGCTCCCCCTACTGACAGTGAAGGCAAAGATG TGGGCAGCACAGAAACAAGCCAAGATGGAGATGCTAGCTCCAGTGAAGGCGAGATGCGGGTCATGGATGA GGACATCATGGTAGAATCAGGTGACGACTCCTGGGATCTGATCACGTGTTACTGCAGAAAGCCCTTTGCG GGGCGGCCCATGATTGAGTGCAGCCTCTGTGGGACGTGGATCCACCTCTCCTGTGCTAAGATTAAGAAGA CCAACGTTCCCGACTTCTTTTATTGCCAGAAATGCAAGGAACTTCGGCCAGAGGCCCGGCGGTTAGGGGG TCTCCCCAAATCTGGAGAACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229306 representing NM_001291125 Red=Cloning site Green=Tags(s) MLEAMAEPSPEDPPPTLKPETQPPEKRRRTIEDFNKFCSFVLAYAGYIPPSKEAPDSATLLEKMKLKDSL FDLDGPKVASPLSPTSLTHTSRPPAALAPVPLSQGDLSQPRKKDRKNRKLGPGGGAGFGVLRRPRPAPGD GEKRSRIKKSKKRKLKKADRGDRLPPPGPPRAPPSDTDSEEEEEEEEEEDDEEEMTVGGGVPAPVLPTPP EAPRPPVTVHSEGAPPTDSEGKDVGSTETSQDGDASSSEGEMRVMDEDIMVESGDDSWDLITCYCRKPFA GRPMIECSLCGTWIHLSCAKIKKTNVPDFFYCQKCKELRPEARRLGGLPKSGEP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291125
ORF Size	1002 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291125.1, NP_001278054.1
RefSeq Size	1818 bp
RefSeq ORF	1005 bp
Locus ID	78246
UniProt ID	Q8BSN5
MW	36.9 kDa
Gene Summary	Acts as a negative regulator of autophagy, through promoting ubiquitination and degradation of LRSAM1, an E3 ubiquitin ligase that promotes autophagy in response to starvation or infecting bacteria.[UniProtKB/Swiss-Prot Function]

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