Nptn (NM_001293673) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AW554172; Sdfr1; SDR-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR228951 representing NM_001293673 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGGCTCGTCGCTCCCCGGCGCCCTGGCCCTCTCGCTCTTGCTGGTCTCTGGCTCGCTCCTTCCAG GGCCAGGCGCTGCTCAGAACGAACCAAGAATTGTCACCAGTGAAGAGGTCATTATTCGAGAAAGCCTCCT TCCTGTCACCCTGCAGTGTAACCTCACTTCCAGCTCTCACACCCTTATGTACAGCTACTGGACAAGGAAT GGGGTAGAACTCACTGCCACCCGTAAGAATGCCAGCAACATGGAGTACAGGATCAATAAGCCAAGAGCTG AGGATTCAGGCGAATACCACTGTGTATATCATTTTGTCAGCGCTCCTAAAGCAAATGCCACCATTGAAGT GAAAGCTGCTCCTGACATCACTGGCCATAAACGAAGTGAAAACAAAAATGAAGGGCAGGATGCTATGATG TACTGCAAGTCAGTTGGCTACCCCCACCCAGAGTGGATATGGCGCAAGAAGGAGAATGGTGTGTTTGAGG AGATTTCTAATAGCTCTGGTCGCTTCTTCATCACCAACAAGGAGAATTACACTGAATTGAGTATTGTGAA TCTCCAAATCACAGAAGATCCTGGAGAGTATGAATGTAATGCCACCAACTCCATTGGCTCTGCCTCCGTT TCCACCGTCCTCAGGGTACGGAGCCACCTTGCCCCACTTTGGCCTTTCTTGGGAATTCTGGCTGAAATCA TCATCCTTGTGGTGATCATTGTTGTGTATGAGAAGAGGAAGAGGCCAGATGAGGTTCCTGATGCTGGGCC AATGAAAACCAACTCTACCAACAATCACAAAGATAAAAACTTGCGCCAGAGAAACACAAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR228951 representing NM_001293673 Red=Cloning site Green=Tags(s) MSGSSLPGALALSLLLVSGSLLPGPGAAQNEPRIVTSEEVIIRESLLPVTLQCNLTSSSHTLMYSYWTRN GVELTATRKNASNMEYRINKPRAEDSGEYHCVYHFVSAPKANATIEVKAAPDITGHKRSENKNEGQDAMM YCKSVGYPHPEWIWRKKENGVFEEISNSSGRFFITNKENYTELSIVNLQITEDPGEYECNATNSIGSASV STVLRVRSHLAPLWPFLGILAEIIILVVIIVVYEKRKRPDEVPDAGPMKTNSTNNHKDKNLRQRNTN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001293673
ORF Size	831 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293673.1, NP_001280602.1
RefSeq Size	2065 bp
RefSeq ORF	834 bp
Locus ID	20320
UniProt ID	P97300
MW	31.3 kDa
Gene Summary	Probable homophilic and heterophilic cell adhesion molecule involved in long term potentiation at hippocampal excitatory synapses through activation of p38MAPK. May also regulate neurite outgrowth by activating the FGFR1 signaling pathway. May play a role in synaptic plasticity (By similarity).[UniProtKB/Swiss-Prot Function]

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