Pitpnb (NM_001301643) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PI-TP-beta
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR228925 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTGATTAAGGAATTCCGTGTGGTTTTGCCGTGTTCTGTTCAAGAGTATCAGGTTGGACAGCTTT ACTCTGTTGCAGAAGCGAGTAAGAATGAAACTGGTGGTGGAGAAGGAATCGAAGTCCTGAAGAATGAACC TTATGAAAACGATGGCGAGAAGGGACAGTACACACACAAAATCTACCACCTGAAGAGCAAAGTTCCTGCA TTTGTGAGGATGATTGCTCCCGAGGGCTCCCTGGTGTTTCATGAGAAAGCCTGGAATGCCTACCCCTACT GCAGAACAATTGTAACGAATGAATACATGAAAGATGACTTCTTCATCAAAATTGAAACATGGCATAAACC TGACTTGGGAACATTAGAAAATGTTCACGGTTTAGATCCCAACACTTGGAAAACTGTTGAAATTGTCCAC ATAGACATTGCAGATCGAAGTCAAGTTGAACCAGCAGACTACAAAGCTGATGAAGACCCTGCATTATTCC ATTCAGTCAAGACCAAGAGAGGACCCCTGGGACCTAACTGGAAGAAGGAGCTGGCAAACACCCCTGACTG TCCTAGGATGTGTGCCTATAAGCTGGTGACCATCAAGTTCAAGTGGTGGGGGCTGCAGAGCAAAGTAGAG AACTTTATCCAGAAGCAAGAAAAACGGATATTTACGAACTTACATCGCCAGCTCTTTTGTTGGATTGACA AGTGGATTGACCTGACAATGGAAGACATTAGGCGAATGGAGGATGAGACTCAGAAAGAACTAGAAACACT ACGGAGTCAAGGCCAAGTGAGGGGGACCAGTGCGGCCTGTGATGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR228925 protein sequence Red=Cloning site Green=Tags(s) MVLIKEFRVVLPCSVQEYQVGQLYSVAEASKNETGGGEGIEVLKNEPYENDGEKGQYTHKIYHLKSKVPA FVRMIAPEGSLVFHEKAWNAYPYCRTIVTNEYMKDDFFIKIETWHKPDLGTLENVHGLDPNTWKTVEIVH IDIADRSQVEPADYKADEDPALFHSVKTKRGPLGPNWKKELANTPDCPRMCAYKLVTIKFKWWGLQSKVE NFIQKQEKRIFTNLHRQLFCWIDKWIDLTMEDIRRMEDETQKELETLRSQGQVRGTSAACDD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001301643
ORF Size	819 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301643.1, NP_001288572.1
RefSeq Size	2762 bp
RefSeq ORF	819 bp
Locus ID	56305
MW	31.6 kDa
Gene Summary	This gene encodes a member of the phosphatidylinositol transfer protein family. The encoded protein catalyzes the transfer of phospholipids (phosphatidylinositol and phosphatidylcholine) between membranes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.