Btg3 (NM_001297747) Mouse Tagged ORF Clone
CAT#: MR228798
- TrueORF®
Btg3 (myc-DDK-tagged) - Mouse B cell translocation gene 3 (Btg3), transcript variant 2
"NM_001297747" in other vectors (1)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,810.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ANA; tob; tob5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR228798 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAACGAAATTGCGGCTGTTGTCTTCTTTTTCACAAGGCTAGTTCGAAAGCATGACAAGTTGAAAA AAGAAGCAGTTGAGAGGTTTGCTGAGAAATTAACTCAAATACTTCAAGAGAAATATAAAAATCACTGGTA TCCAGAAAAACCATCCAAAGGTCAGGCCTACAGATGCATTCGTGTCAATAAGTTTCAGAGAGTTGATCCC GATGTCCTGAAAGCCTGTGAGAACAGCTGCATCTTGTACAGCGACCTGGGCTTGCCTAAGGAGCTTACAC TCTGGGTGGATCCGTGTGAGGTGTGCTGCCGGTATGGAGAGAAAAACAATGCGTTCATTGTTGCCAGCTT TGAAAATGAGGACGAGAACAAGGATGAAATCTCCAAGAAAGTTAGCAGGGCTCTGGATAAGGTGACCTCT GATTATCACTCAGGGTCCTCTTCCTCAGATGAAGACACAAGCAAGGAAGTGGACGTGAAACCCAGCTCAG TGGCGGCAACACCAAGCCCCGTGTACCAGATTTCAGAACTGATATTCCCACCTCTTCCAATGTGGCACCC TTTGCCCAGAAAAAAGCCAGGAATGTATCGAGGGAGCGGCCATCAGACTCACTACCCTCCTCCTGTTCCA TTTGCTTATCCAAATCCAGGAAGGAAGAATAAACCATTCCGCCCAATTCCAGTGACATGGGTACCTCCTC CTGGAATGCATTGTGACCGAAATCACTGGATTAATCCTCACATGTTAGCACCTCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR228798 protein sequence
Red=Cloning site Green=Tags(s) MKNEIAAVVFFFTRLVRKHDKLKKEAVERFAEKLTQILQEKYKNHWYPEKPSKGQAYRCIRVNKFQRVDP DVLKACENSCILYSDLGLPKELTLWVDPCEVCCRYGEKNNAFIVASFENEDENKDEISKKVSRALDKVTS DYHSGSSSSDEDTSKEVDVKPSSVAATPSPVYQISELIFPPLPMWHPLPRKKPGMYRGSGHQTHYPPPVP FAYPNPGRKNKPFRPIPVTWVPPPGMHCDRNHWINPHMLAPH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001297747 |
ORF Size | 759 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001297747.1, NP_001284676.1 |
RefSeq Size | 1218 bp |
RefSeq ORF | 759 bp |
Locus ID | 12228 |
UniProt ID | P50615 |
MW | 29 kDa |
Gene Summary | This gene encodes B cell translocation gene 3, a member of the BTG gene family. This family is defined by a conserved N-terminal domain, known to bind transcription factors, and a less conserved C-terminal domain. This protein is thought to have anti-proliferative properties, and may be involved in regulating the G1-S transition to suppress cell cycle progression. Mice deficient for this gene display an increased incidence of lung cancers, and many human lung cancer cells exhibit decreased levels of B cell translocation gene 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 17. [provided by RefSeq, Jul 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC226587 | Btg3 (untagged) - Mouse B cell translocation gene 3 (Btg3), transcript variant 2 |
CNY 2,570.00 |