Marchf8 (NM_001302385) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1300017E09Rik; M; Marc; MARCH-VIII; March8; Mir
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR228758 representing NM_001302385 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCATCCAAGCAACATTTCTAAGGCTGGGAGTAGTCCTCCATCCACGACGGCTCCAGTGTCTGCCT TCTCTCGCACTTCTGTCACACCATCCAACCAGGACATCTGCAGGATCTGCCACTGTGAAGGGGATGACGA GAGCCCTCTGATCACCCCCTGTCACTGCACAGGGAGCCTCCATTTCGTGCATCAGGCTTGCCTGCAGCAG TGGATCAAGAGTTCTGACACACGCTGCTGTGAACTCTGCAAGTACGAGTTCATCATGGAGACCAAGCTGA AACCTTTGAGGAAATGGGAGAAGTTGCAGATGACTGCCAGTGAGCGCAGGAAGATCATGTGCTCAGTGAC CTTCCATGTCATTGCTATCACCTGTGTGGTCTGGTCCTTGTATGTGCTCATTGACCGCACAGCAGAGGAA ATCAAGCAGGGTCAGGTAACAGGAATCCTAGAGTGGCCTTTCTGGACGAAGCTGGTAGTTGTGGCCATCG GCTTCACTGGAGGACTTCTCTTTATGTATGTTCAGTGCAAGGTGTACCTACAGTTATGGAAAAGACTCAA GGCTTACAATAGAGTGATCTATGTTCAGAACTGTCCAGAAACAAGTAAAAAGAATATTTTTGAAAAGTCT GCACTTACAGAGCCCACCCTTGAAAATAAAGAAGGACATGGAATGTGTCATTCCACCACAAATTCTTCTT GCACAGAGCCTGAAGACACTGGAGCAGAAATTATTAACGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR228758 representing NM_001302385 Red=Cloning site Green=Tags(s) MSHPSNISKAGSSPPSTTAPVSAFSRTSVTPSNQDICRICHCEGDDESPLITPCHCTGSLHFVHQACLQQ WIKSSDTRCCELCKYEFIMETKLKPLRKWEKLQMTASERRKIMCSVTFHVIAITCVVWSLYVLIDRTAEE IKQGQVTGILEWPFWTKLVVVAIGFTGGLLFMYVQCKVYLQLWKRLKAYNRVIYVQNCPETSKKNIFEKS ALTEPTLENKEGHGMCHSTTNSSCTEPEDTGAEIINV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001302385
ORF Size	741 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302385.1, NP_001289314.1
RefSeq Size	4313 bp
RefSeq ORF	744 bp
Locus ID	71779
UniProt ID	Q9DBD2
MW	28.3 kDa
Gene Summary	The protein encoded by this gene is a member of the membrane-associated really interesting new gene-CH family of proteins. These proteins are E3 ubiquitin-protein ligases that modulate antigen presentation by downregulating major histocompatibility complex class II surface expression through endocytosis. The transcript is primarily expressed by dendritic cells and macrophages. Overexpression of this gene in antigen presenting cells results in immune defective phenotypes, including resistance to autoimmune disease onset. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

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