Isg20 (NM_001291221) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	20kDa; 1600023I01Rik; 2010107M23Rik; DnaQL; HEM45
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR228325 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGCATCCCAGAGGTGGTGGCCATGGACTGTGAGATGGTGGGGCTTGGGCCTCAAAGGGTGAGTG GCCTCGCCCGCTGCAGCATTGTGAACATCCATGGCGCAGTCCTGTATGACAAGTACATCCGACCCGAGGG AGAGATCACGGACTACAGAACCCAAGTCAGCGGGGTCACGCCTCAGCACATGGTGAGGGCCACGCCATTT GGTGAAGCCAGGCTAGAGATCCTGCAGCTTCTGAAAGGCAAGCTGGTGGTGGGCCATGACCTGAAGCACG ACTTCAATGCCCTGAAGGAGGATATGAGCAAGTACACCATCTATGACACGTCCACAGACAGGCTGCTGTG GCATGAGGCCAAGCTGCAGTACTACAGCCGAGTGTCCCTGAGGCTGCTGTGTAAGCGCCTGCTACACAAG AACATCCAGAACAACTGGCGGGGCCACTGCTCTGTGGAAGATGCCAGGGCCACAATGGAGCTCTACAAAA TCTCTCAGCGACTCAGAGCCCAGCGAGGGCTGCCTTGCCCTGGGACGTCAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR228325 protein sequence Red=Cloning site Green=Tags(s) MAGIPEVVAMDCEMVGLGPQRVSGLARCSIVNIHGAVLYDKYIRPEGEITDYRTQVSGVTPQHMVRATPF GEARLEILQLLKGKLVVGHDLKHDFNALKEDMSKYTIYDTSTDRLLWHEAKLQYYSRVSLRLLCKRLLHK NIQNNWRGHCSVEDARATMELYKISQRLRAQRGLPCPGTSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291221
ORF Size	546 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291221.1, NP_001278150.1
RefSeq Size	891 bp
RefSeq ORF	546 bp
Locus ID	57444
UniProt ID	Q9JL16
MW	20.6 kDa
Gene Summary	Interferon-induced antiviral exoribonuclease that acts on single-stranded RNA and also has minor activity towards single-stranded DNA. Exhibits antiviral activity against RNA viruses in an exonuclease-dependent manner. May also play additional roles in the maturation of snRNAs and rRNAs, and in ribosome biogenesis (By similarity).[UniProtKB/Swiss-Prot Function]

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