Vegfb (NM_011697) Mouse Tagged ORF Clone
CAT#: MR227639
- TrueORF®
Vegfb (Myc-DDK-tagged) - Mouse vascular endothelial growth factor B (Vegfb), transcript variant 1
ORF Plasmid: tGFP
"NM_011697" in other vectors (4)
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CNY 2,090.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | VEGF-B; Vrf |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR227639 representing NM_011697
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCCCCTGCTCCGTCGCCTGCTGCTTGTTGCACTGCTGCAGCTGGCTCGCACCCAGGCCCCTGTGT CCCAGTTTGATGGCCCCAGCCACCAGAAGAAAGTGGTGCCATGGATAGACGTTTATGCACGTGCCACATG CCAGCCCAGGGAGGTGGTGGTGCCTCTGAGCATGGAACTCATGGGCAATGTGGTCAAACAACTAGTGCCC AGCTGTGTGACTGTGCAGCGCTGTGGTGGCTGCTGCCCTGACGATGGCCTGGAATGTGTGCCCACTGGGC AACACCAAGTCCGAATGCAGATCCTCATGATCCAGTACCCGAGCAGTCAGCTGGGGGAGATGTCCCTGGA AGAACACAGCCAATGTGAATGCAGACCAAAAAAAAAGGAGAGTGCTGTGAAGCCAGACAGGGTTGCCATA CCCCACCACCGTCCCCAGCCCCGCTCTGTTCCGGGCTGGGACTCTACCCCGGGAGCATCCTCCCCAGCTG ACATCATCCATCCCACTCCAGCCCCAGGATCCTCTGCCCGCCTTGCACCCAGCGCCGTCAACGCCCTGAC CCCCGGACCTGCCGCTGCCGCTGCAGACGCCGCCGCTTCCTCCATTGCCAAGGGCGGGGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227639 representing NM_011697
Red=Cloning site Green=Tags(s) MSPLLRRLLLVALLQLARTQAPVSQFDGPSHQKKVVPWIDVYARATCQPREVVVPLSMELMGNVVKQLVP SCVTVQRCGGCCPDDGLECVPTGQHQVRMQILMIQYPSSQLGEMSLEEHSQCECRPKKKESAVKPDRVAI PHHRPQPRSVPGWDSTPGASSPADIIHPTPAPGSSARLAPSAVNALTPGPAAAAADAAASSIAKGGA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_011697 |
ORF Size | 621 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011697.3, NP_035827.1 |
RefSeq Size | 1298 bp |
RefSeq ORF | 624 bp |
Locus ID | 22340 |
UniProt ID | P49766 |
MW | 22.4 kDa |
Gene Summary | Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. VEGF-B seems to be required for normal heart function in adult but is not required for proper development of the cardiovascular system either during development or for angiogenesis in adults.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209638 | Vegfb (untagged) - Mouse vascular endothelial growth factor B (Vegfb), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
MG227639 | Vegfb (tGFP-tagged) - Mouse vascular endothelial growth factor B (Vegfb) transcript variant 1, (10ug) |
CNY 2,280.00 |
|
MR227639L3 | Lenti ORF clone of Vegfb (Myc-DDK-tagged) - Mouse vascular endothelial growth factor B (Vegfb), transcript variant 1 |
CNY 3,990.00 |
|
MR227639L4 | Lenti ORF clone of Vegfb (mGFP-tagged) - Mouse vascular endothelial growth factor B (Vegfb), transcript variant 1 |
CNY 3,990.00 |