Col2a1 (NM_001113515) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Co; Col2; Col2a; Col2a-1; Del; Del1; Dmm; L; Lpk; M100413; Rgsc4; Rgsc8; Rgsc413; Rgsc856
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR227598 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCCGCCTCGGGGCTCCCCAGTCGCTGGTGCTGCTGACGCTGCTCATCGCCGCGGTCCTACGGTGTC AGGGCCAGGATGCCCGAAAATTAGGGCCAAAGGGGCAGAAAGGAGAACCTGGAGATATCAGAGATATCAT AGGACCCAGAGGACCTCCTGGCCCTCAGGGACCTGCAGGTGAACAAGGACCCAGAGGTGATCGTGGTGAC AAGGGAGAAAAGGGTGCGCCTGGACCCCGTGGCAGAGATGGAGAACCTGGTACCCCTGGAAATCCTGGCC CCGCTGGCCCTCCAGGTCCCCCTGGTCCCCCTGGCCTTAGTGCAGGAAACTTCGCGGCTCAGATGGCTGG AGGGTATGACGAGAAGGCTGGTGGTGCCCAGATGGGAGTCATGCAAGGGCCCATGGGCCCCATGGGACCC CGTGGACCCCCAGGCCCTGCCGGTGCCCCCGGCCCTCAAGGATTTCAAGGCAATCCTGGTGAACCTGGCG AGCCTGGTGTCTCTGGTCCCATGGGTCCCCGAGGTCCTCCTGGCCCTGCTGGAAAACCTGGTGACGACGG TGAAGCTGGGAAGCCCGGAAAGTCTGGGGAAAGAGGCCTCCCTGGCCCTCAGGGTGCTCGTGGATTCCCA GGAACCCCGGGTCTCCCCGGTGTCAAGGGTCACAGAGGTTACCCAGGCCTCGACGGTGCTAAGGGGGAAG CTGGTGCTCCGGGTGTGAAGGGTGAGAGTGGTTCCCCTGGTGAGAACGGATCCCCGGGCCCAATGGGTCC CCGTGGCCTGCCTGGTGAGAGAGGACGGACTGGCCCTGCTGGTGCTGCTGGTGCTCGGGGTAACGATGGC CAGCCAGGCCCCGCTGGACCTCCGGGTCCTGTGGGTCCCGCAGGTGGTCCTGGCTTCCCTGGTGCTCCTG GTGCCAAGGGCGAAGCTGGTCCCACTGGTGCTCGCGGTCCTGAAGGTGCTCAAGGTTCTCGTGGCGAGCC TGGCAATCCTGGGTCCCCTGGGCCTGCAGGTGCTTCTGGTAACCCAGGGACTGATGGTATTCCTGGAGCC AAAGGATCCGCTGGTGCTCCTGGAATTGCTGGTGCCCCTGGCTTCCCTGGGCCCCGTGGCCCTCCCGGTC CTCAAGGTGCAACTGGTCCCCTTGGCCCCAAAGGTCAGGCGGGTGAACCTGGCATTGCTGGCTTTAAAGG TGATCAAGGCCCCAAGGGAGAGACTGGACCTGCTGGGCCCCAAGGAGCCCCTGGCCCCGCTGGTGAAGAA GGCAAACGAGGTGCTCGAGGAGAGCCGGGTGGTGCTGGACCAATCGGACCCCCTGGAGAGAGAGGTGCTC CTGGCAACCGTGGATTCCCAGGTCAAGATGGTCTGGCAGGTCCCAAGGGTGCCCCTGGAGAGCGAGGGCC CAGTGGCTTGACTGGTCCCAAGGGAGCCAACGGTGACCCGGGTCGTCCTGGAGAACCTGGTCTTCCTGGA GCCAGGGGTCTTACCGGTCGCCCTGGTGACGCTGGTCCTCAAGGCAAAGTTGGTCCTTCTGGAGCCCCTG GTGAAGACGGTCGCCCTGGACCTCCTGGTCCTCAGGGAGCTCGTGGGCAGCCTGGCGTCATGGGTTTCCC TGGCCCCAAAGGTGCCAACGGCGAGCCTGGCAAAGCTGGTGAGAAGGGTCTGGCTGGCGCTCCTGGTCTG AGAGGTCTTCCTGGAAAAGACGGTGAGACGGGAGCCGCAGGACCCCCCGGCCCCAGTGGACCTGCTGGTG AACGAGGCGAGCAGGGCGCTCCTGGACCATCAGGGTTCCAGGGACTTCCTGGCCCTCCCGGTCCCCCAGG TGAAGGTGGAAAGCAAGGTGACCAGGGTATTCCTGGTGAAGCTGGAGCTCCTGGCCTTGTGGGTCCTCGG GGCGAGCGAGGTTTCCCAGGTGAACGTGGCTCTCCTGGTGCTCAGGGCCTTCAGGGTCCCCGAGGCCTCC CTGGCACTCCTGGTACTGATGGTCCCAAAGGTGCAGCTGGCCCAGATGGCCCCCCTGGGGCTCAGGGGCC TCCAGGTCTACAGGGAATGCCTGGTGAGAGAGGAGCCGCTGGCATTGCTGGGCCCAAGGGAGACAGAGGC GATGTTGGCGAGAAAGGCCCAGAGGGAGCTCCTGGGAAGGATGGCGGCCGAGGTCTGACTGGGCCCATCG GACCCCCAGGCCCAGCAGGGGCCAACGGCGAGAAGGGAGAAGTCGGACCTCCTGGCCCGTCAGGAAGTAC CGGAGCTCGAGGTGCCCCGGGTGAACGCGGAGAGACCGGGCCACCTGGACCTGCTGGATTCGCTGGCCCT CCTGGTGCTGATGGCCAGCCTGGTGCCAAGGGTGATCAAGGAGAAGCCGGACAGAAAGGAGATGCTGGTG CCCCCGGCCCACAAGGCCCCTCGGGAGCCCCTGGGCCACAGGGTCCTACTGGAGTGACTGGTCCTAAGGG AGCCCGAGGTGCCCAAGGTCCCCCGGGAGCCACCGGATTCCCTGGAGCTGCTGGCCGAGTTGGACCCCCA GGTGCTAATGGCAATCCTGGACCCGCCGGTCCCCCTGGTCCTGCTGGAAAAGATGGTCCCAAAGGTGTTC GAGGAGACAGTGGCCCCCCTGGCAGAGCTGGTGACCCCGGTCTTCAAGGTCCTGCAGGAGCTCCTGGCGA GAAAGGAGAACCTGGAGATGATGGTCCCTCTGGTCTTGATGGTCCTCCAGGTCCCCAGGGGCTGGCTGGT CAAAGGGGCATTGTTGGTCTGCCTGGTCAGCGTGGTGAGAGAGGATTCCCCGGCCTTCCCGGCCCATCGG GTGAGCCCGGCAAGCAGGGTGCACCTGGCGCGTCTGGAGACAGAGGTCCTCCTGGTCCTGTGGGGCCTCC TGGCCTGACAGGGCCTGCAGGTGAACCTGGACGAGAGGGCAGCCCTGGTGCTGATGGACCCCCTGGAAGA GATGGTGCAGCTGGAGTCAAGGGAGATCGTGGTGAGACTGGAGCACTGGGTGCCCCTGGAGCTCCTGGGC CCCCAGGCTCTCCTGGTCCTGCTGGCCCAACTGGCAAACAAGGAGACAGAGGAGAGGCTGGTGCACAAGG TCCTATGGGTCCCTCAGGACCTGCTGGAGCCCGTGGGATTGCAGGCCCTCAAGGCCCCCGAGGTGACAAA GGAGAATCTGGAGAGCAGGGCGAGAGGGGACTGAAGGGACACCGAGGTTTCACTGGACTGCAGGGTCTGC CTGGCCCTCCGGGTCCTTCTGGAGATCAGGGTGCTTCTGGCCCTGCTGGTCCTTCTGGCCCTAGAGGTCC ACCTGGCCCTGTTGGTCCCTCTGGCAAAGATGGCTCTAATGGAATCCCTGGCCCCATCGGGCCTCCAGGT CCCCGTGGACGCTCAGGAGAAACAGGCCCTGTTGGTCCCCCTGGAAGTCCCGGTCCTCCTGGCCCTCCAG GTCCTCCTGGTCCTGGCATCGACATGTCAGCCTTTGCTGGCTTAGGGCAGAGAGAGAAGGGCCCCGACCC CATGCAGTACATGCGGGCCGACGAGGCAGACAGTACCTTGAGACAGCACGACGTGGAGGTGGACGCTACA CTCAAGTCACTGAACAACCAGATTGAGAGCATCCGCAGCCCCGACGGCTCCCGCAAGAACCCTGCTCGCA CTTGCCAAGACCTGAAACTCTGCCACCCCGAGTGGAAGAGCGGAGACTACTGGATTGATCCCAACCAGGG CTGCACCTTGGACGCCATGAAAGTTTTCTGCAACATGGAGACCGGCGAGACTTGCGTCTACCCCAACCCA GCGACTGTCCCTCGGAAAAACTGGTGGAGCAGCAAGAGCAAGGAAAAGAAACACATCTGGTTTGGAGAGA CCATGAACGGTGGCTTCCACTTCAGCTATGGCGATGGCAACCTGGCTCCCAACACCGCTAACGTCCAGAT GACTTTCCTCCGTCTACTGTCCACTGAGGGCTCCCAGAACATCACCTACCACTGTAAGAACAGCATCGCC TACCTGGACGAAGCGGCTGGCAACCTCAAGAAGGCCTTGCTCATCCAGGGCTCCAATGATGTAGAGATGA GGGCCGAGGGCAACAGCAGGTTCACATACACTGCCCTGAAGGATGGCTGCACGAAACACACTGGTAAGTG GGGCAAGACCGTCATCGAGTACCGATCACAGAAGACCTCCCGCCTTCCCATTATTGACATCGCACCCATG GACATTGGAGGGGCTGAACAGGAATTTGGTGTGGACATAGGGCCTGTCTGCTTCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR227598 protein sequence Red=Cloning site Green=Tags(s) MIRLGAPQSLVLLTLLIAAVLRCQGQDARKLGPKGQKGEPGDIRDIIGPRGPPGPQGPAGEQGPRGDRGD KGEKGAPGPRGRDGEPGTPGNPGPAGPPGPPGPPGLSAGNFAAQMAGGYDEKAGGAQMGVMQGPMGPMGP RGPPGPAGAPGPQGFQGNPGEPGEPGVSGPMGPRGPPGPAGKPGDDGEAGKPGKSGERGLPGPQGARGFP GTPGLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLPGERGRTGPAGAAGARGNDG QPGPAGPPGPVGPAGGPGFPGAPGAKGEAGPTGARGPEGAQGSRGEPGNPGSPGPAGASGNPGTDGIPGA KGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQAGEPGIAGFKGDQGPKGETGPAGPQGAPGPAGEE GKRGARGEPGGAGPIGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPSGLTGPKGANGDPGRPGEPGLPG ARGLTGRPGDAGPQGKVGPSGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLAGAPGL RGLPGKDGETGAAGPPGPSGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKQGDQGIPGEAGAPGLVGPR GERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGAAGPDGPPGAQGPPGLQGMPGERGAAGIAGPKGDRG DVGEKGPEGAPGKDGGRGLTGPIGPPGPAGANGEKGEVGPPGPSGSTGARGAPGERGETGPPGPAGFAGP PGADGQPGAKGDQGEAGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPP GANGNPGPAGPPGPAGKDGPKGVRGDSGPPGRAGDPGLQGPAGAPGEKGEPGDDGPSGLDGPPGPQGLAG QRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGASGDRGPPGPVGPPGLTGPAGEPGREGSPGADGPPGR DGAAGVKGDRGETGALGAPGAPGPPGSPGPAGPTGKQGDRGEAGAQGPMGPSGPAGARGIAGPQGPRGDK GESGEQGERGLKGHRGFTGLQGLPGPPGPSGDQGASGPAGPSGPRGPPGPVGPSGKDGSNGIPGPIGPPG PRGRSGETGPVGPPGSPGPPGPPGPPGPGIDMSAFAGLGQREKGPDPMQYMRADEADSTLRQHDVEVDAT LKSLNNQIESIRSPDGSRKNPARTCQDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNP ATVPRKNWWSSKSKEKKHIWFGETMNGGFHFSYGDGNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIA YLDEAAGNLKKALLIQGSNDVEMRAEGNSRFTYTALKDGCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPM DIGGAEQEFGVDIGPVCFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001113515
ORF Size	4257 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001113515.2, NP_001106987.2
RefSeq Size	4944 bp
RefSeq ORF	4260 bp
Locus ID	12824
UniProt ID	P28481
MW	134.7 kDa
Gene Summary	This gene encodes the alpha-1 subunit of the fibril-forming type II collagen, the major component of cartilage and the vitreous humor of the eye. The encoded preproprotein forms homotrimeric, triple helical procollagen that undergoes proteolytic processing during fibirl formation. Mice harboring certain mutations in this gene exhibit severe chondrodysplasia characterized by short limbs and trunch, craniofacial deformities and cleft palate. A complete lack of the encoded protein in mice results in postnatal lethality. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Dec 2015]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.