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Agap1 (NM_178119) Mouse Tagged ORF Clone
CAT＃: MR227525
- TrueORF®
Agap1 (Myc-DDK-tagged) - Mouse ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant 1
ORF Plasmid: tGFP
"NM_178119" in other vectors (4)
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CNY 8,550.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Centg2; Ggap1; mKIAA1099; R75364 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227525 representing NM_178119
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTACCAGCAGCAGCTGGCCAACTCGGCTGCCATCCGGGCCGAGATCCAGCGCTTCGAGTCGGTCC ACCCCAACATCTACTCCATCTACGAGCTGCTGGAGCGCGTGGAGGAGCCGGTGCTGCAGAACCAGATTCG GGAGCACGTCATCGCCATCGAAGATGCTTTCGTGAACAGCCAGGAATGGACACTCAGCCGATCGGTACCG GAGCTCAAAGTGGGAATTGTGGGTAACCTGGCCAGTGGCAAGTCTGCGCTGGTGCACCGGTACCTGACAG GCACGTACGTGCAGGAGGAATCTCCAGAAGGTGGCAGGTTCAAGAAGGAGATTGTTGTTGATGGACAGAG TTATCTGCTGCTGATTAGGGATGAAGGGGGCCCCCCGGAGGCACAGTTCGCCATGTGGGTGGACGCGGTC ATCTTTGTCTTCAGCTTGGAGGATGAGATCAGTTTCCAGACTGTCTACCATTACTACAGCCGAATGGCCA ACTACAGGAACACCAGTGAGATCCCATTAGTGCTGGTGGGGACCCAGGATGCCATAAGTTCCACCAACCC GAGGGTCATCGATGACGTCAGGGCTCGGAAGCTCTCCAATGACCTGAAGAGGTGCACGTACTATGAGACG TGCGCCACCTATGGGCTCAACGTGGAGCGGGTCTTCCAGGACGTTGCCCAGAAGATCGTTGCCACAAGGA AGAAGCAGCAGCTATCCATAGGCCCCTGCAAGTCCTTACCCAACTCTCCAAGCCACTCGTCTGTGTGTTC TGCCCAAGTGTCCGCGGTACACATCAGCCAGACAAGTAACGGAGGTGGGAGTTTAAGTGACTACTCATCC TCTGTTCCTTCGACCCCAAGCACCAGCCAGAAAGAGCTTCGGATCGACGTCCCTCCCACTGCCAACACTC CAACGCCTGTCCGGAAGCAGTCCAAGCGCAGGTCCAACCTATTCACATCTAGGAAAGGGAGCGACCCAGA CAAAGAGAAGAAAGGCCTGGAGAGCCGTGCGGACAGCATCGGGAGCGGGCGAGCCATCCCAATTAAACAG GGCATGCTGCTGAAGCGAAGTGGCAAGTCACTGAACAAAGAGTGGAAAAAGAAGTACGTCACCCTGTGCG ACAACGGTGTGCTGACCTACCACCCCAGTTTACATGATTACATGCAGAATGTCCACGGCAAAGAGATAGA CCTCTTGAGGACCACTGTGAAGGTGCCGGGGAAGAGGCCGCCCCGAGCCACGTCTGCCTGTGCACCGATC TCCAGTCCCAAAACCAACGGCCTGGCCAAGGACATGAGCAGTTTACACATCTCACCAAATTCAGGGAATG TCACTAGTGCGTCTGGGTCTCAGATGGCAAGCGGCATCAGCCTGGTCTCCTTCAACAGCCGACCCGATGG CATGCACCAGCGCTCCTACTCAGTCTCCAGTGCCGACCAGTGGAGTGACGCTACAGTCATTGCAAACTCA GCCATCAGCAGTGACACGGGGCTGGGTGACTCGGTGTGTTCCAGCCCAAGTATCTCCAGCAGCACCAGCC CCAAGCTCGACCCACCCCCCTCCCCCCACGCCAACAGAAAGAAGCACCGGAGGAAGAAAAGTACCAGCAA CTTCAAGGCTGACGGGCTCTCAGGCACTGCTGAAGAGCAAGAAGAAAACTTGGAGTTTATCATTGTGTCC CTCACTGGCCAGACGTGGCACTTTGAAGCCACCACATACGAGGAGCGAGACGCATGGGTCCAAGCCATCG AGAGCCAGATCCTCGCCAGCCTGCAGTCGTGCGAGAGCAGCAAGAATAAGTCCCGACTAACCAGCCAGAG CGAAGCCATGGCCCTGCAGTCCATCCGTAACATGAGAGGGAACTCGCACTGCGTGGACTGTGACACCCAA AACCCTAACTGGGCCAGTTTGAACTTGGGAGCCCTCATGTGTATCGAGTGCTCGGGGATCCACCGGAATC TCGGCACCCACCTCTCTCGGGTCAGATCGCTAGACCTGGACGACTGGCCCATGGAGCTGATCAAGGTGAT GTCATCCATCGGGAATGAGCTGGCCAACAGCGTGTGGGAAGAGGGCAGCCAGGGACGGACAAAGCCCTCA CTGGACTCCACCAGGGAAGAAAAGGAAAGGTGGATCCGGGCCAAGTATGAGCAGAAACTCTTCCTGGCCC CACTGCCATGCACAGAGTTCTCCCTGGGCCAGCAGCTGCTGCGTGCCACTGCCGAGGAAGACCTGCGGAC TGTCATCCTACTTCTGGCACATGGGTCCCGGGACGAGGTGAATGAGACCTGTGGGGAAGGAGACGGTCGC ACGGCGCTGCACCTGGCCTGTCGCAAGGGCAACGTGGTCCTGGCTCAGCTGCTGATCTGGTACGGAGTGG ACGTCATGGCCCGAGACGCCCACGGGAACACAGCACTGGCCTATGCCCGGCAGGCCTCCAGCCAGGAGTG TATTGACGTGCTTCTACAGTATGGCTGCCCCGACGAGCGCTTTGTGCTCATGGCTACCCCTAACCTGTCC AGGAAGAGCAACAGCAGGAACAACAGTAGCGGGAGGGCGCCCAGTGTCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227525 representing NM_178119
Red=Cloning site Green=Tags(s) MNYQQQLANSAAIRAEIQRFESVHPNIYSIYELLERVEEPVLQNQIREHVIAIEDAFVNSQEWTLSRSVP ELKVGIVGNLASGKSALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAV IFVFSLEDEISFQTVYHYYSRMANYRNTSEIPLVLVGTQDAISSTNPRVIDDVRARKLSNDLKRCTYYET CATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHSSVCSAQVSAVHISQTSNGGGSLSDYSS SVPSTPSTSQKELRIDVPPTANTPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLESRADSIGSGRAIPIKQ GMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSACAPI SSPKTNGLAKDMSSLHISPNSGNVTSASGSQMASGISLVSFNSRPDGMHQRSYSVSSADQWSDATVIANS AISSDTGLGDSVCSSPSISSSTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLSGTAEEQEENLEFIIVS LTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNKSRLTSQSEAMALQSIRNMRGNSHCVDCDTQ NPNWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPMELIKVMSSIGNELANSVWEEGSQGRTKPS LDSTREEKERWIRAKYEQKLFLAPLPCTEFSLGQQLLRATAEEDLRTVILLLAHGSRDEVNETCGEGDGR TALHLACRKGNVVLAQLLIWYGVDVMARDAHGNTALAYARQASSQECIDVLLQYGCPDERFVLMATPNLS RKSNSRNNSSGRAPSVI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178119 |
| ORF Size | 2571 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178119.3, NP_835220.1 |
| RefSeq Size | 9581 bp |
| RefSeq ORF | 2574 bp |
| Locus ID | 347722 |
| UniProt ID | Q8BXK8 |
| MW | 94.9 kDa |
| Gene Summary | GTPase-activating protein for ARF1 and, to a lesser extent, ARF5. Directly and specifically regulates the adapter protein 3 (AP-3)-dependent trafficking of proteins in the endosomal-lysosomal system (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222206 | Agap1 (untagged) - Mouse ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant 1, (10ug) |
CNY 10,930.00 |
|
| MG227525 | Agap1 (tGFP-tagged) - Mouse ArfGAP with GTPase domain ankyrin repeat and PH domain 1 (Agap1) transcript variant 1, (10ug) |
CNY 9,410.00 |
|
| MR227525L3 | Lenti ORF clone of Agap1 (Myc-DDK-tagged) - Mouse ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant 1 |
CNY 10,450.00 |
|
| MR227525L4 | Lenti ORF clone of Agap1 (mGFP-tagged) - Mouse ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant 1 |
CNY 10,450.00 |
