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Ppp1r15a (NM_008654) Mouse Tagged ORF Clone
CAT＃: MR227514
- TrueORF®
Ppp1r15a (Myc-DDK-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 15A (Ppp1r15a)
ORF Plasmid: tGFP
"NM_008654" in other vectors (4)
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CNY 4,896.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9630030H21; Gadd34; Myd116 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227514 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCGAGCCCAAGACCCCAGCATGTCCTGCACTGGAGGGACGCCCACAACTTCTATCTCCTGTCCC CACTGATGGGCTTGCTCAGTCGGGCCTGGAGCCGCCTGAGGGGCCCAGAAGTCCCAGAGGCATGGCTGGC AAAAACAGTAACAGGAGCAGATCAGATAGAAGCTGCGGCTCTGCTGACACCTACCCCTGTCTCTGGTAAC CTCCTCCCTCATGGGGAGACTGAAGAAAGTGGATCTCCTGAACAGAGTCAAGCAGCCCAGAGGCTCTGCC TTGTGGAAGCTGAAAGTTCCCCTCCTGAAACTTGGGGACTTTCAAATGTTGATGAGTACAATGCAAAGCC AGGACAAGATGACCTTAGAGAGAAGGAAATGGAACGCACAGCTGGCAAGGCCACACTACAGCCCGCTGGC CTGCAAGGGGCTGATAAGAGGCTTGGGGAGGTGGTGGCTAGAGAAGAGGGAGTGGCTGAGCCCGCTTATC CCACATCACAGCTGGAGGGTGGTCCAGCTGAGAATGAAGAGGATGGAGAAACAGTGAAGACTTACCAAGC TTCTGCTGCTTCCATAGCTCCGGGATACAAACCCAGCACCCCTGTGCCTTTCTTGGGGGAGGCAGAACAT CAAGCCACGGAAGAAAAAGGAACAGAAAACAAGGCTGACCCCTCCAACTCTCCTTCTTCAGGCTCCCACT CCAGAGCCTGGGAGTACTACTCTAGAGAGAAGCCTAAGCAGGAGGGAGAAGCCAAGGTAGAGGCACACAG GGCAGGGCAGGGTCACCCTTGTCGGAATGCTGAGGCTGAGGAAGGAGGACCTGAGACAACTTTTGTCTGT ACTGGAAATGCCTTCCTGAAGGCCTGGGTGTATCGCCCAGGAGAGGACACAGAGGAAGAAGACAACAGCG ATTCGGATTCAGCTGAGGAAGACACAGCTCAGACCGGTGCCACCCCCCATACAAGTGCCTTCCTGAAGGC CTGGGTGTATCGCCCAGGAGAGGACACAGAGGAAGAAGACAGCGATTCGGATTCAGCTGAGGAAGACACA GCTCAGACCGGTGCCACCCCCCATACAAGTGCCTTCCTGAAGGCCTGGGTGTATCGCCCAGGAGAGGACA CAGAGGAAGAAAACAGCGATTTGGATTCAGCTGAGGAAGACACAGCTCAGACCGGTGCCACCCCCCATAC AAGTGCCTTCCTGAAGGCCTGGGTGTATCGCCCAGGAGAGGACACAGAGGAAGAAAACAGCGATTTGGAT TCAGCTGAGGAAGACACAGCTCAGACCGGTGCCACCCCACATACAAGTCCCTTCCTGAAGGCCTGGGTGT ATCGCCCAGGAGAGGACACAGAAGATGACACAGAAGAGGAAGAGGACAGTGAGAATGTGGCCCCAGGTGA CTCAGAAACAGCTGACTCAAGCCAGAGTCCCTGCCTTCAGCCCCAGCGTTGTCTACCAGGAGAGAAGACC AAGGGACGTGGGGAAGAGCCCCCTCTCTTCCAGGTGGCCTTCTATTTACCCGGAGAGAAGCCAGAATCAC CTTGGGCTGCACCTAAGCTGCCCCTTCGACTGCAGAGGCGGCTCAGATTGTTCAAAGCCCCCACCCGGGA TCAGGACCCCGAGATTCCTCTAAAAGCTCGGAAGGTACACTTCGCTGAGAAAGTCACAGTCCATTTCCTT GCTGTCTGGGCAGGACCAGCCCAAGCTGCCCGTCGAGGTCCCTGGGAGCAGTTTGCACGAGATCGAAGCC GCTTTGCTCGACGCATTGCCCAGGCAGAGGAGAAGCTGGGTCCCTACCTTACCCCTGATTCCAGGGCCAG AGCATGGGCACGCCTTAGAAACCCATCTCTTCCACAGTCCGAGCCTCGCTCTTCCTCTGAGGCCACTCCC TTGACCCAAGATGTGACCACACCCTCTCCCCTTCCCAGTGAAACCCCTTCGCCCAGCCTGTACTTGGGAG GGAGGCGGGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227514 protein sequence
Red=Cloning site Green=Tags(s) MAPSPRPQHVLHWRDAHNFYLLSPLMGLLSRAWSRLRGPEVPEAWLAKTVTGADQIEAAALLTPTPVSGN LLPHGETEESGSPEQSQAAQRLCLVEAESSPPETWGLSNVDEYNAKPGQDDLREKEMERTAGKATLQPAG LQGADKRLGEVVAREEGVAEPAYPTSQLEGGPAENEEDGETVKTYQASAASIAPGYKPSTPVPFLGEAEH QATEEKGTENKADPSNSPSSGSHSRAWEYYSREKPKQEGEAKVEAHRAGQGHPCRNAEAEEGGPETTFVC TGNAFLKAWVYRPGEDTEEEDNSDSDSAEEDTAQTGATPHTSAFLKAWVYRPGEDTEEEDSDSDSAEEDT AQTGATPHTSAFLKAWVYRPGEDTEEENSDLDSAEEDTAQTGATPHTSAFLKAWVYRPGEDTEEENSDLD SAEEDTAQTGATPHTSPFLKAWVYRPGEDTEDDTEEEEDSENVAPGDSETADSSQSPCLQPQRCLPGEKT KGRGEEPPLFQVAFYLPGEKPESPWAAPKLPLRLQRRLRLFKAPTRDQDPEIPLKARKVHFAEKVTVHFL AVWAGPAQAARRGPWEQFARDRSRFARRIAQAEEKLGPYLTPDSRARAWARLRNPSLPQSEPRSSSEATP LTQDVTTPSPLPSETPSPSLYLGGRRG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008654 |
| ORF Size | 1974 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008654.2, NP_032680.1 |
| RefSeq Size | 2345 bp |
| RefSeq ORF | 1974 bp |
| Locus ID | 17872 |
| UniProt ID | P17564 |
| MW | 71.8 kDa |
| Gene Summary | Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. In case of infection with vesicular stomatitis virus (VSV), impairs viral replication.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220161 | Ppp1r15a (untagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 15A (Ppp1r15a), (10ug) |
CNY 8,460.00 |
|
| MG227514 | Ppp1r15a (tGFP-tagged) - Mouse protein phosphatase 1 regulatory (inhibitor) subunit 15A (Ppp1r15a), (10ug) |
CNY 5,040.00 |
|
| MR227514L3 | Lenti ORF clone of Ppp1r15a (Myc-DDK-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 15A (Ppp1r15a) |
CNY 6,460.00 |
|
| MR227514L4 | Lenti ORF clone of Ppp1r15a (mGFP-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 15A (Ppp1r15a) |
CNY 6,460.00 |
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