Bco1 (NM_001163028) Mouse Tagged ORF Clone
CAT#: MR227449
- TrueORF®
Bcmo1 (Myc-DDK-tagged) - Mouse beta-carotene 15,15'-monooxygenase (Bcmo1), transcript variant 2
ORF Plasmid: tGFP
"NM_001163028" in other vectors (4)
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CNY 3,920.00
CNY 4,560.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Bcd; Bcdo; Bcdo1; Bcmo; Bcmo1; beta-C; beta-CD; betaCM; betaCMOOX; Cm; CMO1; Cmoi |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR227449 representing NM_001163028
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGATAATATTTGGCCAGAATAAGAAAGAACAGCTGGAGCCAGTTCAGGCCAAAGTGACAGGCAGCA TTCCAGCATGGCTGCAGGGGACCCTGCTCCGAAACGGGCCCGGGATGCACACAGTGGGAGAGAGCAAGTA CAACCATTGGTTTGATGGCCTGGCCCTTCTCCACAGTTTCTCCATCAGAGATGGGGAGGTCTTCTACAGG AGCAAATACCTGCAGAGTGACACCTACATCGCCAACATTGAGGCCAACAGAATCGTGGTGTCTGAGTTCG GAACCATGGCCTACCCGGACCCCTGCAAAAACATCTTTTCCAAAGCTTTCTCCTACTTGTCTCACACCAT CCCCGACTTCACAGACAACTGTCTGATCAACATCATGAAATGTGGAGAAGACTTCTATGCAACCACGGAG ACCAACTACATCAGGAAAATCGACCCCCAGACCCTAGAGACCTTGGAGAAGGTTGATTACCGGAAGTATG TGGCGGTAAACCTGGCTACCTCGCACCCTCATTATGACGAGGCTGGGAATGTCCTTAACATGGGCACATC CGTCGTGGACAAAGGGAGGACAAAATACGTGATATTTAAGATCCCTGCCACAGTGCCAGACAGCAAGAAG AAAGGGAAGAGTCCCGTGAAGCACGCGGAAGTTTTCTGCTCCATTTCCTCCCGCTCGTTGCTCTCTCCCA GCTACTACCACAGCTTTGGTGTCACGGAGAACTATGTGGTGTTTCTGGAGCAGCCTTTTAAGTTGGATAT CCTCAAGATGGCCACCGCATACATGAGGGGAGTGAGCTGGGCTTCCTGTATGTCATTCGACAGGGAGGAC AAGACATACATTCATATCATCGACCAGAGGACCAGGAAGCCTGTGCCTACCAAGTTCTACACAGATCCCA TGGTGGTCTTCCATCATGTCAATGCCTACGAGGAGGACGGCTGTGTGCTGTTTGATGTGATCGCCTATGA GGACAGCAGCCTCTATCAGCTCTTCTACCTGGCCAACCTGAACAAGGACTTCGAGGAGAAGTCCAGGCTG ACCTCAGTGCCTACCCTCAGGAGGTTTGCTGTGCCCCTCCATGTGGACAAGGATGCAGAAGTGGGCTCAA ATTTAGTCAAGGTGTCATCTACAACTGCAACAGCCCTGAAGGAGAAAGACGGCCATGTCTATTGCCAGCC CGAGGTCCTCTACGAAGGCCTAGAGCTCCCTCGGATAAATTATGCTTACAACGGGAAGCCATATCGCTAC ATCTTTGCAGCTGAAGTACAGTGGAGTCCAGTCCCAACCAAGATACTGAAATATGACATTCTCACAAAGT CCTCCTTAAAGTGGTCTGAGGAGAGCTGCTGGCCAGCAGAGCCTCTGTTTGTTCCCACGCCAGGTGCGAA GGATGAAGATGATGTCCCTTGCAGGCCAAGCAGAGACTGTGGAGTCCAAGGGCCCCATCGTAAATCACAT TGTCAGCTGAGCATAGCATGTGTGCCTGAGATCTCAGCATTTGGGAGGCAGACAGAAGACAATCAGGGGT CTACAGTCATTCTCAGATATACAGAGAATTTGAACTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227449 representing NM_001163028
Red=Cloning site Green=Tags(s) MEIIFGQNKKEQLEPVQAKVTGSIPAWLQGTLLRNGPGMHTVGESKYNHWFDGLALLHSFSIRDGEVFYR SKYLQSDTYIANIEANRIVVSEFGTMAYPDPCKNIFSKAFSYLSHTIPDFTDNCLINIMKCGEDFYATTE TNYIRKIDPQTLETLEKVDYRKYVAVNLATSHPHYDEAGNVLNMGTSVVDKGRTKYVIFKIPATVPDSKK KGKSPVKHAEVFCSISSRSLLSPSYYHSFGVTENYVVFLEQPFKLDILKMATAYMRGVSWASCMSFDRED KTYIHIIDQRTRKPVPTKFYTDPMVVFHHVNAYEEDGCVLFDVIAYEDSSLYQLFYLANLNKDFEEKSRL TSVPTLRRFAVPLHVDKDAEVGSNLVKVSSTTATALKEKDGHVYCQPEVLYEGLELPRINYAYNGKPYRY IFAAEVQWSPVPTKILKYDILTKSSLKWSEESCWPAEPLFVPTPGAKDEDDVPCRPSRDCGVQGPHRKSH CQLSIACVPEISAFGRQTEDNQGSTVILRYTENLNY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001163028 |
ORF Size | 1578 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001163028.1, NP_001156500.1 |
RefSeq Size | 2532 bp |
RefSeq ORF | 1581 bp |
Locus ID | 63857 |
MW | 60.3 kDa |
Gene Summary | Vitamin A metabolism is important for vital processes such as vision, embryonic development, cell differentiation, and membrane and skin protection. The protein encoded by this gene is a key enzyme in beta-carotene metabolism to vitamin A. It catalyzes the oxidative cleavage of beta,beta-carotene into two retinal molecules. Two alternatively spliced variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217460 | Bcmo1 (untagged) - Mouse beta-carotene 15,15'-monooxygenase (Bcmo1), transcript variant 2, (10ug) |
CNY 6,750.00 |
|
MG227449 | Bcmo1 (tGFP-tagged) - Mouse beta-carotene 1515'-monooxygenase (Bcmo1) transcript variant 2, (10ug) |
CNY 5,040.00 |
|
MR227449L3 | Lenti ORF clone of Bcmo1 (Myc-DDK-tagged) - Mouse beta-carotene 15,15'-monooxygenase (Bcmo1), transcript variant 2 |
CNY 6,460.00 |
|
MR227449L4 | Lenti ORF clone of Bcmo1 (mGFP-tagged) - Mouse beta-carotene 15,15'-monooxygenase (Bcmo1), transcript variant 2 |
CNY 6,460.00 |