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Mark2 (NM_007928) Mouse Tagged ORF Clone
CAT＃: MR227294
- TrueORF®
Mark2 (Myc-DDK-tagged) - Mouse MAP/microtubule affinity-regulating kinase 2 (Mark2), transcript variant 1
ORF Plasmid: tGFP
"NM_007928" in other vectors (3)
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CNY 7,700.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Emk; EMK-1; Par-1; Par-1b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227294 representing NM_007928
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGCGCTCGGACCCCCCTACCCACGCTGAACGAAAGGGACACGGAGCAGCCCACCTTGGGACACC TTGATTCCAAGCCCAGCAGTAAGTCCAACATGCTGCGGGGCCGCAACTCAGCCACCTCTGCTGACGAGCA GCCCCATATTGGCAACTACCGGCTCCTTAAGACCATTGGCAAGGGTAACTTTGCCAAGGTGAAGTTGGCC CGGCACATCCTGACGGGGAAAGAGGTAGCTGTGAAGATCATCGACAAGACCCAGCTGAACTCCTCCAGCC TACAGAAACTGTTCCGAGAAGTAAGAATAATGAAGGTTTTGAATCATCCCAACATAGTTAAGTTGTTTGA AGTGATCGAGACTGAGAAGACTCTCTACCTTGTCATGGAGTATGCCAGTGGCGGAGAGGTGTTTGATTAC CTAGTGGCCCATGGCAGGATGAAAGAAAAAGAAGCTCGAGCCAAATTTCGCCAGATAGTGTCTGCTGTGC AGTACTGTCACCAGAAGTTCATTGTTCATAGAGATCTAAAGGCAGAAAACCTGCTCCTGGATGCTGATAT GAACATCAAGATTGCAGACTTTGGCTTTAGCAACGAATTCACCTTTGGGAACAAGCTGGATACTTTCTGT GGCAGTCCTCCTTATGCTGCCCCAGAACTTTTCCAGGGCAAAAAGTATGATGGTCCTGAGGTGGATGTCT GGAGCCTGGGAGTCATCCTCTATACACTGGTCAGCGGATCCCTGCCTTTTGATGGACAGAACCTCAAGGA GCTGCGGGAACGGGTACTGAGGGGGAAATACCGTATTCCGTTCTACATGTCCACGGACTGTGAAAATCTG CTTAAGAAATTTCTCATACTTAATCCTAGTAAGAGAGGCACTTTAGAGCAAATTATGAAAGATCGGTGGA TGAACGTGGGGCATGAGGACGATGAGCTAAAGCCTTATGTGGAACCTCTCCCTGACTACAAGGACCCCCG GCGGACAGAGTTGATGGTGTCAATGGGTTACACACGGGAAGAGATCCAGGACTCGCTGGTAGGCCAGAGG TACAACGAAGTGATGGCTACCTATCTGCTCCTTGGCTACAAGAGCTCTGAGCTGGAAGGTGATACCATCA CTTTGAAGCCCCGGCCTTCAGCTGATCTAACCAACAGCAGTGCCCCATCTCCATCCCACAAGGTTCAGCG CAGCGTCTCTGCCAACCCCAAGCAACGACGCTCCAGTGACCAGGCCGTCCCTGCCATTCCCACCTCGAAT TCCTACTCTAAGAAGACTCAGAGTAACAACGCAGAAAATAAGCGGCCTGAGGAAGAGACAGGGCGGAAAG CCAGCAGCACCGCCAAAGTGCCTGCCAGCCCTCTGCCTGGCCTGGACAGGAAGAAGACCACTCCTGCCCC CTCCACGAACAGCGTCCTTTCCACCAGCACAAACCGAAGCAGGAACTCCCCACTTTTGGACAGGGCCAGC CTTGGCCAGGCCTCCATCCAGAATGGTAAAGACAGCCTAACCATGCCAGGGTCCCGGGCCTCCACGGCTT CTGCTTCTGCCGCAGTCTCTGCGGCCCGGCCCCGCCAGCACCAGAAATCCATGTCTGCCTCCGTACACCC CAACAAGGCCTCTGGGTTGCCCCCCACGGAGAGTAACTGTGAGGTGCCTCGGCCCAGCACAGCCCCCCAG CGCGTCCCTGTCGCCTCCCCCTCCGCCCACAACATCAGCAGCAGTAGTGGAGCCCCAGACCGAACTAATT TCCCACGGGGTGTGTCCAGTCGAAGCACCTTCCATGCTGGGCAGCTCCGACAGGTGCGGGACCAGCAGAA TCTACCCTACGGTGTGACCCCAGCCTCTCCCTCTGGCCATAGCCAGGGCCGGCGGGGGGCCTCTGGCAGC ATCTTCAGCAAGTTCACCTCCAAGTTTGTCCGCAGGAACCTGAATGAACCTGAAAGCAAAGACCGAGTGG AGACGCTCAGACCTCACGTGGTAGGCAGTGGAGGCACTGACAAGGACAAGGAGGAGTTTCGGGAGGCCAA GCCTCGCTCCCTGCGCTTCACCTGGAGCATGAAGACCACGAGCTCTATGGAGCCCAATGAGATGATGCGG GAGATCCGCAAGGTGCTGGACGCCAACAGCTGCCAAAGCGAGCTGCACGAGCGGTACATGCTACTGTGCG TGCATGGCACACCAGGCCACGAGAACTTTGTGCAGTGGGAGATGGAGGTGTGCAAACTGCCCCGGCTGTC TCTCAACGGTGTTCGGTTTAAGCGGATATCGGGCACTTCCATGGCCTTCAAAAACATTGCCTCCAAAATA GCCAATGAGCTGAAGCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227294 representing NM_007928
Red=Cloning site Green=Tags(s) MSSARTPLPTLNERDTEQPTLGHLDSKPSSKSNMLRGRNSATSADEQPHIGNYRLLKTIGKGNFAKVKLA RHILTGKEVAVKIIDKTQLNSSSLQKLFREVRIMKVLNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDY LVAHGRMKEKEARAKFRQIVSAVQYCHQKFIVHRDLKAENLLLDADMNIKIADFGFSNEFTFGNKLDTFC GSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENL LKKFLILNPSKRGTLEQIMKDRWMNVGHEDDELKPYVEPLPDYKDPRRTELMVSMGYTREEIQDSLVGQR YNEVMATYLLLGYKSSELEGDTITLKPRPSADLTNSSAPSPSHKVQRSVSANPKQRRSSDQAVPAIPTSN SYSKKTQSNNAENKRPEEETGRKASSTAKVPASPLPGLDRKKTTPAPSTNSVLSTSTNRSRNSPLLDRAS LGQASIQNGKDSLTMPGSRASTASASAAVSAARPRQHQKSMSASVHPNKASGLPPTESNCEVPRPSTAPQ RVPVASPSAHNISSSSGAPDRTNFPRGVSSRSTFHAGQLRQVRDQQNLPYGVTPASPSGHSQGRRGASGS IFSKFTSKFVRRNLNEPESKDRVETLRPHVVGSGGTDKDKEEFREAKPRSLRFTWSMKTTSSMEPNEMMR EIRKVLDANSCQSELHERYMLLCVHGTPGHENFVQWEMEVCKLPRLSLNGVRFKRISGTSMAFKNIASKI ANELKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007928 |
| ORF Size | 2328 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007928.3 |
| RefSeq Size | 4648 bp |
| RefSeq ORF | 2331 bp |
| Locus ID | 13728 |
| UniProt ID | Q05512 |
| MW | 86.8 kDa |
| Gene Summary | Serine/threonine-protein kinase. Involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4 and RAB11FIP2. Phosphorylates the microtubule-associated protein MAPT/TAU. Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227294 | Mark2 (tGFP-tagged) - Mouse MAP/microtubule affinity-regulating kinase 2 (Mark2) transcript variant 1, (10ug) |
CNY 8,460.00 |
|
| MR227294L3 | Lenti ORF clone of Mark2 (Myc-DDK-tagged) - Mouse MAP/microtubule affinity-regulating kinase 2 (Mark2), transcript variant 1 |
CNY 9,600.00 |
|
| MR227294L4 | Lenti ORF clone of Mark2 (mGFP-tagged) - Mouse MAP/microtubule affinity-regulating kinase 2 (Mark2), transcript variant 1 |
CNY 9,600.00 |
