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Dnajc3 (NM_008929) Mouse Tagged ORF Clone
CAT＃: MR227024
- TrueORF®
Dnajc3 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 3 (Dnajc3)
ORF Plasmid: tGFP
"NM_008929" in other vectors (4)
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CNY 6,000.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA408985; AU067833; Dnajc3a; Dnajc3b; mp58; p58; p58IPK; Prkri |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227024 representing NM_008929
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGCCCCCGGCTCGGTGGGCAGCCGGCTGGGCGCGGTGTTCCCGTTCCTGCTGGTCCTGGTGGACC TGCAGTACGAAGGCGCTGAGTGTGGAGTAAATGCGGATGTGGAGAAGCATCTTGAATTGGGGAAGAAATT ACTCGCAGCCGGACAGCTAGCCGACGCCTTATCACAGTTTCACGCTGCAGTTGATGGTGACCCCGATAAC TATATCGCATACTATAGGAGAGCTACCGTCTTCTTAGCCATGGGAAAATCCAAAGCAGCGCTGCCCGACC TCACCAAAGTGATTGCGCTGAAGATGGACTTTACTGCCGCAAGACTACAGAGAGGTCACCTACTGCTCAA ACAAGGGAAGCTTGACGAAGCAGAAGACGATTTCAAGAAAGTGCTCAAATCTAACCCCAGTGAGCAGGAG GAGAAGGAAGCCGAGTCTCAGCTTGTAAAAGCTGACGAGATGCAGCGGCTGCGCTCCCAAGCGCTCGACG CCTTTGACGGTGCCGATTACACTGCTGCTATAACCTTCCTTGATAAGATTTTAGAGGTTTGTGTTTGGGA TGCAGAACTGCGTGAACTGCGAGCAGAATGCTTCATAAAGGAAGGAGAGCCCAGGAAAGCCATCAGCGAC TTAAAGGCTGCTTCAAAGCTGAAGAGTGACAACACAGAGGCCTTTTACAAAATCAGCACACTCTACTACC AGCTAGGAGACCATGAGCTGTCTCTCAGTGAAGTTCGTGAATGTCTGAAACTTGACCAGGATCACAAGCG GTGTTTTGCACACTATAAGCAAGTGAAGAAACTTAACAAGCTGATTGAGTCAGCTGAAGAGCTGATCCGA GATGGCAGATACACAGATGCAACCAGCAAATATGAATCAGTCATGAAAACAGAGCCCAGTGTTGCTGAGT ACACAGTGCGCTCGAAGGAGAGGATCTGCCACTGCTTCTCTAAGGATGAGAAGCCCGTGGAAGCCATTAG GATATGTTCTGAAGTTTTACAGATGGAGCCCGACAATGTGAATGCTCTGAAAGACCGAGCCGAGGCCTAC TTAATAGAGGAAATGTACGATGAAGCCATTCAGGATTATGAAGCTGCTCAGGAGCACAATGAAAATGACC AGCAGATTCGGGAAGGCTTAGAGAAAGCCCAGCGGTTACTGAAGCAGTCACAGAAACGAGATTATTATAA AATCTTGGGAGTAAAAAGAAATGCCAAAAAACAAGAAATCATTAAAGCATACCGAAAGTTAGCACTGCAG TGGCATCCAGATAATTTCCAGAATGAAGAAGAAAAGAAAAAAGCAGAGAAAAAGTTCATTGACATAGCAG CTGCTAAAGAAGTCCTCTCAGACCCAGAAATGAGAAAGAAGTTTGATGATGGAGAAGATCCTCTTGATGC AGAGAGTCAACAAGGAGGTGGTGGCAACCCCTTCCACAGAAGTTGGAACTCATGGCAAGGGTTTAACCCC TTCAGCTCAGGCGGACCGTTCAGATTTAAGTTCCACTTCAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227024 representing NM_008929
Red=Cloning site Green=Tags(s) MVAPGSVGSRLGAVFPFLLVLVDLQYEGAECGVNADVEKHLELGKKLLAAGQLADALSQFHAAVDGDPDN YIAYYRRATVFLAMGKSKAALPDLTKVIALKMDFTAARLQRGHLLLKQGKLDEAEDDFKKVLKSNPSEQE EKEAESQLVKADEMQRLRSQALDAFDGADYTAAITFLDKILEVCVWDAELRELRAECFIKEGEPRKAISD LKAASKLKSDNTEAFYKISTLYYQLGDHELSLSEVRECLKLDQDHKRCFAHYKQVKKLNKLIESAEELIR DGRYTDATSKYESVMKTEPSVAEYTVRSKERICHCFSKDEKPVEAIRICSEVLQMEPDNVNALKDRAEAY LIEEMYDEAIQDYEAAQEHNENDQQIREGLEKAQRLLKQSQKRDYYKILGVKRNAKKQEIIKAYRKLALQ WHPDNFQNEEEKKKAEKKFIDIAAAKEVLSDPEMRKKFDDGEDPLDAESQQGGGGNPFHRSWNSWQGFNP FSSGGPFRFKFHFN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008929 |
| ORF Size | 1512 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008929.3, NP_032955.2 |
| RefSeq Size | 5190 bp |
| RefSeq ORF | 1515 bp |
| Locus ID | 100037258 |
| UniProt ID | Q91YW3 |
| MW | 57.5 kDa |
| Gene Summary | Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein synthesis under ER stress, hypothermic and amino acid starving stress conditions (PubMed:25329545). Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217248 | Dnajc3 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 3 (Dnajc3), (10ug) |
CNY 6,560.00 |
|
| MG227024 | Dnajc3 (tGFP-tagged) - Mouse DnaJ (Hsp40) homolog subfamily C member 3 (Dnajc3), (10ug) |
CNY 5,510.00 |
|
| MR227024L3 | Lenti ORF clone of Dnajc3 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 3 (Dnajc3) |
CNY 6,940.00 |
|
| MR227024L4 | Lenti ORF clone of Dnajc3 (mGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 3 (Dnajc3) |
CNY 6,940.00 |
