Rp1 (NM_011283) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Dcdc3; Gm38717; mG145; O; Orp1; Rp; Rp1h
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR226962 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGACACACCTTCTACTAGTTTCTCCATGATTCATCTGACTTCTGAAGGTCAAGTTCCTTCCCCTC GCCATTCAAATATCACTCATCCTGTAGTGGCTAAACGCATCAGTTTCTATAAGAGTGGAGACCCACAGTT TGGCGGCGTTCGGGTGGTGGTCAACCCTCGTTCCTTTAAGACTTTTGACGCTCTGCTGGACAGTTTATCC AGGAAGGTACCCCTGCCCTTTGGGGTAAGGAACATCAGCACGCCCCGTGGACGACACAGCATCACCAGGC TGGAGGAGCTAGAGGACGGCAAGTCTTATGTGTGCTCCCACAATAAGAAGGTGCTGCCAGTTGACCTGGA CAAGGCCCGCAGGCGCCCTCGGCCCTGGCTGAGTAGTCGCTCCATAAGCACGCATGTGCAGCTCTGTCCT GCAACTGCCAATATGTCCACCATGGCACCTGGCATGCTCCGTGCCCCAAGGAGGCTCGTGGTCTTCCGGA ATGGTGACCCGAAGAATAAGCATGTGGTCCTTCTTAGCCGGAGGATCACTCAGAGCTTCGAAGCCTTTCT GCAGTACCTGACACAGGTTATGCAGTGTCCTGTGGCCAAGCTGTATGCCACAGATGGAAGAAAAGTTCCT AGTCTCCAGGCAGTGATACTCAGCTCTGGAGCTGTGGTGGCAGCTGGAAGGGAGCCATTTAAACCAGGAA ATTACGACATACAAAAGTACTTGCTTCCTGCCAAGTTACCAGGAATCTCTCATCGTGTGCACCAGAAGGG AAAAGCCAAGATAGAAAAAAGAAAGATGAGCACACATATGCCTTCAGACCTAAGACCCCAGACTGATTCC CTGATCTCGGAGAAAACATATGATTGCTTTTCAGATTTTTCTGTAGCCCCTGAGAATTACTTGGCCTTAG AAACACATGAGTCTCAGTCTCTATCAACTTATCCTTCTGAAGATGATGTTGAGAAATCGATTGTTTTTAA TCAAGATGGTACGATGACGGTCGTGATGAAAGTTCGATTCAAGATAAAAGAAGAAGAAACTGTTAAGTGG ACAACAACTGTCAATAGAGCTGGTCTTTCTAATAATGATGAAAAGAATAAGAAAAGTAGTTATCCTGGAA AAACAGATTATGGACCATCTAGTTTAAAGCTTGAAGCATGTTCGTTGCCTGAAGACATCGTAGATACCAC TCAGCAGGGCAGTTTGACAGAGGAAGAAAATACTCAAATGACAGAGCAGCAGGCTCTGTCATGTAGCTCT GCTAGTTGGGAGAATGCCTCTATGGAGACAGACATTCAGGAAAGCCAGAAACAAGTGAAACACTTTTATA GACCCCCAACTCCTGGACCAAGGAGAATGAGACAAAAGAAATCTGTGATAGGGACTGTCACAGTAGTATC TGAAACAGAGGTTCAAGAAAAGCAGTTTTCCTATAGTGAAGAAAGAAAGGGTGGAGAAAAATCTGAGTAT CATATGTTTACACATTCTTGCAGTAAAATGTCATCAGTATCTAATAAACTTGTTCAGATCGGTAGCGATA ATGAGATGGAGTCAGCATTAGAAAGAACAAGGGAAAGTGGATCACTCAAGTCACAGGCCATAAATGCTGG TGCTATTGAAATTACAAGTCAGAAGGTCTTAAAGATGTGCCACAACAATGCTTTACCGTCAACTGCACCA GAGAACTCAGTCGTGGAAGAAGGCACAGACAACAGTGCAGTATCAGGCACAGCCACTATCAAGCACTTCA GAACTTGTGGTAATGCCAATGACAGCTTCAGTTCTATCACAGCAGATTCAACTCCTACCTCAGTTAACAA TTATTCAAATGACAGAAATATTTCTGAGCTTCCTTCTGTAGGATCTCCTGTACTCACCATGAGACTTGTT AATGAATTTGCTCATTGTGGTTTAACAGAAAAACCAGAAAACAGAAAGAAGGTTTTATCTTCTTCTGCTA GCAAGAAGAAGAAAAAGAAATCTCAGCAGCGAATGATAACTTCCAATGATAAGAAAAAAGTGATTGAAAC CAAAGGACCCCCAAATATAGCTGGGAAGATACCCAGAGCTGGAACAACTGCACAGGAAAGACTATTGCAA GAATCAGACTGTCCTGATAAAGGAGGAAGAGTTTGTGAGCAGGGCCTTAATATAAGTCCTATGGCAATTG AATCAAATAATTTTTTTCCCAAAAGTAATCCCACATTTTCTAAGAATTTCTATAAAAATAAATTAAATAC TTTTCAAAATCCTAAGACTCAAAAACTTTTGGCCAAAAGAAAATCAAGGCCACGAAAGATTGTAAGCACA GAAAGACTCAGAAAACAAGAGATTGGTCAAGAAGATAAAATACTTCTCCATAGTGACTCGAAACTCTGTG AAAGTCATTTGGAAAAACAAAGTTTATTTCATGTGTTTAATATCCTTGAAGAAGATCAGAAAGTTTTGCA CAGACCACCGTTCCAAGTGGAGAAAGTAGCTAGAAATTTGAAAGGAATGGCAAAAAAGAGTTTAGTCCCA AAAGTCAATGATTTACACATAATGTTAAGAAACCAGAAAAAACAAATGGGAGTTAAATTAAAGTCAGGTG CTGAGGTAAGTGAACAGCATGTTACAACCAGGGCAGATCCATTAGCTTCTCTGAAAAAACCTGATTTTCC TGAGGGCATTCCCCATCATTCAGGTAAAAGCTATGTAAAGCGATGGTTGCAGAACATAAACTCATATCCA GATTTTGAGCATAGAAAGTCCGGTCCACTATGCCAAAATAGAAGTGATGTGGTAAATTATAACCGCAATG GCTTTCTAGGAAATAATCTCCATACAACTTCTTCAAAAGGAAATGGTTTTTTAATGGAAAGTAATAAGAG CAAAACTAAAAACGACAATTGGTCAGGCAATACAAATCAAGAGACAGGTAAATCTCTTGTTGCTAAAGAT AATGGTGAAGAGCTCAATAAACATCACTGTGAGAGCCAGAATGGGTCTCTGTATGATTCTTACTTGGTTT CTTTGCATGACAATTGTACTTTGTCACAGACCACTATTAATGAGCCTAGTACTAAAAGTCATTTATCTAT TGAAAAGTCAAGACCAGAAGTAAAGCTTGTTTACCAAGAAATGAACTTTGCTACAAAAAGGCAAAGTATT GAAGTTGCCATTCAGGTAGATACTATGGGAGAAAATGTTCTAAAAGACTACTTACCAGCCTTGTTGCTAC GGCACCTGGAAGCTTTTGTTCCTAATAATCAGAAACATCAGAATGGAATTTCTCAAATACCAGGTTCATT AGCAGAGGTTGTCTTCCCTTCTGTAATAGATAATTCATCCACAAATCTCCTTCTGGCTTGGCTTTTGGTA CTAAACCTAAAGAGAACTATGAATAGCTTCTGTCAAAGTGATGCTCATAAGATGACCAACAGACCTTCGG AAACTGCTGCATTGTTGGAAGTTCTGAAGCATGTTGCCATCACAGAGGAAGCTGATGACTTGAAGGCTGC TGTTGCCAACTTGATGGAATCAACCAAAACTTGCAGTGGGTCCAGTGGGAGAGAACAAGATATGCTACCA GTAAATTGTACAGCATCCAGCCTTCATAGTGTGGACGAGTGTAATGAAAATGGGAGTGCTCAGAAGACCC TCTTGGATGAAGGCTATTCTGTCATGGGGGATTGTACCTCAGAAATGGTTAGCAAGTCATGTAACTCCTC ATGTGAGATGCACATGGTGAGCAAAACTAATCCTCCAAAACAGGTAGATGACCAGAGTGATGGCCTTCTT ACTAGCAATAGCTGCACTGTGAGTCAGCGCTCCACAGGGGCCTGTTTCCTTACAGATGGGGTGTATTCCC ATGAGGCTTGTGCTCAGAAGGAGGGCGTCTATGAGGGAGCTTGCCTAAGTGATGAGACTCACATTCCTAT CAGAGACTGCCATACAATTCACTCTGTGCATTCAAAAGAAAACAAATGCACTGATGATCTGGAATCAACT GAAGAACTCAAAACTGTTGACAAAGTTCCAAAAGGTCTAAGCATTCTGGCAGATTCTATGTATAAAAATG ACTCTAATGTATCTACATTTCAAAATGTCAATAAGTTAAGTTCCCAACGCACTCTCCTAAGTAAAACTTA TCTAGACTCTGATAAGGACTATAGTCCTCTAGAAGAATTTCAAAATTGCCCACGTAAGAAAATTGTGAAT AAAAAGAAATCTATTTCTTCTGATAAGGAAGAATCAAGGACTTCTGAGGAACCAAGGTCAATAACCAATA GCATGACATCAAGTGAAAGAAATGCCATTTCAGAATTAGAATCTTTTGAAGAATTAGAAAGCCAAGACAC AAGTATCTTCAATATGAATGTAAGGGCTGAAAAAAAATCTACCAAGGAAACGATGCAAAAACAATCAGAG GCTAGGATGAGTTCAGAATTGATAAACGTATCAGGGAGGAAAATTATAGAACAGGAAAGAAGAAATACTG CCATTCTGGAGACAACCGCTAGGGGGCAGGTGACACCACCATCTTTAGCTTTTTGCTACGATTCTAACAA AAACACCGAAAAAGAGATCAGTGAAGGAGAAACTAAGATGAGAGTTAAGAAGATGGTAGACAGCATGGAA AATGAAAGTTACTCGGAGTCCTCACTTAATTTTAAAAAACATCACAGAAGTCCAGGAACCTTAGACTGGT CAGACTATGGATCAGATAGTGAGAGTGGATATCCTTGTAAAGCATCGAGCAATAGTCACAATGATGACTC TGGCCAAGAGAAAGAGCCCACAAGAGGAATTGTTAAAAGAGCAATAGAAAAGCTTTATGGCAAAGCAGAG ATTATCAAACCACCATTTTTCCATGGGTCTATACACAAATCTCAAGTTTGTCCTTATAATTCTGTGGAAG TTCAGTGTGCTAAGAAAACCAATTTTTATGAGTCTGAATGCCAGTCATTGGTCTCTTCTGAACAGGTATC CAGAAGTTCACTCATATTTCAGGAATTCCCTCAGGTTGATGCTAATGGCATGGGAGACAGTTTTGGGGAC AGCAGCATAGAAAATGTTACAAAATCAAGTGCGCATGACAGGGTCTTTACAGAAAAAGAGAATGGAAAAC TGATTGACAATGGCAAATGGCTCCTGAGAGAAAATCATTTGTGGCGGGTGTCATCGGACAATCCTGGCAT GTACGGCAATGCAGACACCACATCAGTGGATACTCTAATAGATAAGAACAGCATTGAGGTGCCATATTCA CATTTCGGAGAGCTGGCTCCTGGTCCAACAATGGCTGAACTGTCCTCTTCAGAAATAGAGGAAATGACTC AACCCCTTGAAGTGAAATGCAATTATTTTAACTTCCCTCATGGTAGTGACTCTGAGCCTTTTGGTGAAGA TTTTCCAGATGCACAGAATAAAACTTGTCCCAAAGAGAAAATACCCAACCATCACACAGAGGAGAAGGGT AATTATCCATCGGAAAGACTGTGTACATCTGTTACTCAAGCCTTTGTTTCTGCTGGTAATAAAGTCCACC CGGTCTGCAGTGACGCTATTAAAACTCAGCCATTACCTGGGAGTAACATCACTCACGGCGCACTTCAGGA AGGTGACTCTTTGGATAAACTCTATGCTCTTTGTGGTCAACATTGTCCGATACTAACTGTTATTATTCAA CCTGTAAATGAGGAAAGCCGAGGATTTGCTTATCGCAAAGATTCTGATATTGAAAATTCTTTGGATTTTC AGTTATGGATGAAAATATACCCATTTATGCCACAGTCAAAGAAACACGTGTTTAGAAGTGATGGCAGGAA TGTGAGTGTGGGTGAAGAATTTGCTGGTAATGTCATTGGTGATCTCTGTGATCAACTTTATTTCAAAAGC ATGATTGACTTAGTGGATCAAAGAGCTAACTCTTTAGGGAAGGAAATTAATTTGAAGAAATTCCAACTAT ATTTAAAGAAAAGCTTTTCCGATCCTTTGTCCACGTCATTGCTAGTGGTGGAGAATAGGAATTCTGTTTC ACTAAGCCCTAGCAGTTGGACAGATAACTTCAAAAGTATTGATGAGAATAACAACTTCCTTAACAGATTA CCGAACTCAAGTAAAAATCCCAACCAAGTAGTAAGAGAGAACACTAATTTTCAGTTCCACTTAGAACTGT TTGGTCAAGTTTACTTGTTAGATATTTGCCAAGTTGAGAAACCCTTAAATATCAAAACCAGAAGTAAATT AGAAATGTATTACATTTTAGAGGGTGAAGTTCTTTTCATCTGGGAAGAAGAAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR226962 protein sequence Red=Cloning site Green=Tags(s) MSDTPSTSFSMIHLTSEGQVPSPRHSNITHPVVAKRISFYKSGDPQFGGVRVVVNPRSFKTFDALLDSLS RKVPLPFGVRNISTPRGRHSITRLEELEDGKSYVCSHNKKVLPVDLDKARRRPRPWLSSRSISTHVQLCP ATANMSTMAPGMLRAPRRLVVFRNGDPKNKHVVLLSRRITQSFEAFLQYLTQVMQCPVAKLYATDGRKVP SLQAVILSSGAVVAAGREPFKPGNYDIQKYLLPAKLPGISHRVHQKGKAKIEKRKMSTHMPSDLRPQTDS LISEKTYDCFSDFSVAPENYLALETHESQSLSTYPSEDDVEKSIVFNQDGTMTVVMKVRFKIKEEETVKW TTTVNRAGLSNNDEKNKKSSYPGKTDYGPSSLKLEACSLPEDIVDTTQQGSLTEEENTQMTEQQALSCSS ASWENASMETDIQESQKQVKHFYRPPTPGPRRMRQKKSVIGTVTVVSETEVQEKQFSYSEERKGGEKSEY HMFTHSCSKMSSVSNKLVQIGSDNEMESALERTRESGSLKSQAINAGAIEITSQKVLKMCHNNALPSTAP ENSVVEEGTDNSAVSGTATIKHFRTCGNANDSFSSITADSTPTSVNNYSNDRNISELPSVGSPVLTMRLV NEFAHCGLTEKPENRKKVLSSSASKKKKKKSQQRMITSNDKKKVIETKGPPNIAGKIPRAGTTAQERLLQ ESDCPDKGGRVCEQGLNISPMAIESNNFFPKSNPTFSKNFYKNKLNTFQNPKTQKLLAKRKSRPRKIVST ERLRKQEIGQEDKILLHSDSKLCESHLEKQSLFHVFNILEEDQKVLHRPPFQVEKVARNLKGMAKKSLVP KVNDLHIMLRNQKKQMGVKLKSGAEVSEQHVTTRADPLASLKKPDFPEGIPHHSGKSYVKRWLQNINSYP DFEHRKSGPLCQNRSDVVNYNRNGFLGNNLHTTSSKGNGFLMESNKSKTKNDNWSGNTNQETGKSLVAKD NGEELNKHHCESQNGSLYDSYLVSLHDNCTLSQTTINEPSTKSHLSIEKSRPEVKLVYQEMNFATKRQSI EVAIQVDTMGENVLKDYLPALLLRHLEAFVPNNQKHQNGISQIPGSLAEVVFPSVIDNSSTNLLLAWLLV LNLKRTMNSFCQSDAHKMTNRPSETAALLEVLKHVAITEEADDLKAAVANLMESTKTCSGSSGREQDMLP VNCTASSLHSVDECNENGSAQKTLLDEGYSVMGDCTSEMVSKSCNSSCEMHMVSKTNPPKQVDDQSDGLL TSNSCTVSQRSTGACFLTDGVYSHEACAQKEGVYEGACLSDETHIPIRDCHTIHSVHSKENKCTDDLEST EELKTVDKVPKGLSILADSMYKNDSNVSTFQNVNKLSSQRTLLSKTYLDSDKDYSPLEEFQNCPRKKIVN KKKSISSDKEESRTSEEPRSITNSMTSSERNAISELESFEELESQDTSIFNMNVRAEKKSTKETMQKQSE ARMSSELINVSGRKIIEQERRNTAILETTARGQVTPPSLAFCYDSNKNTEKEISEGETKMRVKKMVDSME NESYSESSLNFKKHHRSPGTLDWSDYGSDSESGYPCKASSNSHNDDSGQEKEPTRGIVKRAIEKLYGKAE IIKPPFFHGSIHKSQVCPYNSVEVQCAKKTNFYESECQSLVSSEQVSRSSLIFQEFPQVDANGMGDSFGD SSIENVTKSSAHDRVFTEKENGKLIDNGKWLLRENHLWRVSSDNPGMYGNADTTSVDTLIDKNSIEVPYS HFGELAPGPTMAELSSSEIEEMTQPLEVKCNYFNFPHGSDSEPFGEDFPDAQNKTCPKEKIPNHHTEEKG NYPSERLCTSVTQAFVSAGNKVHPVCSDAIKTQPLPGSNITHGALQEGDSLDKLYALCGQHCPILTVIIQ PVNEESRGFAYRKDSDIENSLDFQLWMKIYPFMPQSKKHVFRSDGRNVSVGEEFAGNVIGDLCDQLYFKS MIDLVDQRANSLGKEINLKKFQLYLKKSFSDPLSTSLLVVENRNSVSLSPSSWTDNFKSIDENNNFLNRL PNSSKNPNQVVRENTNFQFHLELFGQVYLLDICQVEKPLNIKTRSKLEMYYILEGEVLFIWEEEK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_011283
ORF Size	6288 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_011283.2, NP_035413.1
RefSeq Size	7508 bp
RefSeq ORF	6288 bp
Locus ID	19888
UniProt ID	P56716
MW	234.4 kDa
Gene Summary	This gene encodes a member of the doublecortin family. The protein encoded by this gene contains two doublecortin domains, which bind microtubules and regulate microtubule polymerization. The encoded protein is a photoreceptor microtubule-associated protein and is required for correct stacking of outer segment disc. This protein and the RP1L1 protein, another retinal-specific protein, play essential and synergistic roles in affecting photosensitivity and outer segment morphogenesis of rod photoreceptors. Because of its response to in vivo retinal oxygen levels, this protein was initially named ORP1 (oxygen-regulated protein-1). This protein was subsequently designated RP1 (retinitis pigmentosa 1) when it was found that mutations in this gene cause autosomal dominant retinitis pigmentosa. Mutations in this gene also cause autosomal recessive retinitis pigmentosa. [provided by RefSeq, Jun 2019]

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