Myh4 (NM_010855) Mouse Tagged ORF Clone
CAT#: MR226919
- TrueORF®
Myh4 (Myc-DDK-tagged) - Mouse myosin, heavy polypeptide 4, skeletal muscle (Myh4)
ORF Plasmid: tGFP
"NM_010855" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 13,040.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI506973; MHC2B; Minimsc; Minmus; MM; MYH-2B; MyHC-IIb; Myhsf |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226919 representing NM_010855
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCCGACGCTGAGATGGCCGTTTTCGGGGAGGCTGCTCCTTACCTCCGGAAGTCTGAAAAGGAGC GAATCGAGGCCCAGAACAAGCCTTTTGATGCCAAGTCATCGGTGTTTGTGGTGGATGCTAAGGAGTCCTA TGTGAAAGCAACAGTGCAGAGCAGGGAAGGGGGGAAGGTGACAGCCAAGACCGAAGGCGGAGCTACGGTC ACCGTCAAGGATGATCAAGTCTTCTCCATGAACCCTCCCAAGTACGACAAGATTGAGGACATGGCCATGA TGACCCACCTGCACGAGCCCGCTGTGCTGTACAACCTCAAAGAGCGTTATGCAGCCTGGATGATCTACAC TTACTCAGGCCTCTTTTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAACCCTGAGGTGGTG GCAGCCTACCGAGGCAAAAAGCGCCAGGAGGCCCCACCCCACATCTTCTCCATCTCTGATAACGCCTACC AGTTCATGCTGACGGATCGTGAGAATCAGTCCATCCTGATTACTGGAGAATCCGGGGCCGGGAAGACTGT GAACACGAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTCACTGGGGACAAGAAGAAGGAGGAGGCG ACTTCTGGCAAAATGCAGGGGACCCTTGAAGATCAAATTATCAGTGCCAACCCCCTGCTGGAGGCCTTCG GCAACGCCAAGACTGTGAGGAACGACAACTCTTCTCGATTTGGTAAATTCATCAGAATCCATTTCGGTGC CACAGGCAAACTGGCCTCTGCAGATATTGAAACCTATCTGCTAGAGAAGTCCAGAGTCACCTTCCAGCTC AAGGCTGAAAGGAGCTACCACATCTTCTACCAAATCATGTCCAATAAGAAACCAGAGCTTATTGAGATGC TTCTGATCACCACCAACCCATATGACTTTGCTTACGTCAGTCAAGGTGAAATCACAGTGCCCAGCATTGA TGACCAGGAAGAGCTGATGGCCACAGACACTGCTGTTGATATCCTGGGATTCAGTGCAGATGAAAAGGTG GCCATTTACAAGCTCACAGGCGCTGTGATGCATTATGGGAACATGAAATTCAAGCAAAAGCAAAGGGAAG AGCAGGCTGAGCCAGACGGCACCGAAGTTGCTGACAAGGCTGCCTATCTGACAAGTCTGAACTCTGCTGA CCTGCTCAAAGCCCTCTGCTACCCCAGGGTCAAGGTCGGCAATGAGTATGTCACCAAAGGCCAGACGGTG CAGCAGGTGTACAACTCCGTGGGTGCCCTGGCCAAGTCCATGTATGAGAAGATGTTCCTATGGATGGTCA CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCCGGCAGTACTTCATCGGGGTCTTGGACATTGCTGG CTTTGAGATCTTTGACTTCAACACCTTGGAGCAGCTGTGCATCAACTTCACCAATGAGAAACTGCAACAG TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAGGGCATTGATTGGGAGTTCA TTGACTTCGGGATGGACCTGGCGGCCTGCATCGAGCTCATCGAGAAGCCGATGGGCATCTTCTCCATCCT GGAAGAGGAGTGCATGTTCCCTAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTATGAGCAGCATCTT GGAAAGTCCAACAACTTCCAGAAGCCCAAACCCGCCAAAGGCAAGGCTGAGGCTCACTTCTCCCTGGTGC ACTATGCGGGCACCGTGGACTACAACATTATTGGCTGGCTGGACAAGAACAAGGACCCCCTGAATGAGAC CGTGGTGGGGCTGTACCAGAAATCCGGGTTGAAGACTCTGGCTTTCCTATTTTCTGGGGGACAAGCTGCG GAAGCAGAGGGCGGCGGTGGAAAGAAAGGTGGCAAGAAGAAGGGTTCTTCTTTCCAGACCGTGTCAGCTC TCTTCAGGGAGAATTTAAATAAGCTGATGACCAACTTGAAGAGCACCCACCCCCACTTTGTCAGATGCCT CATTCCCAATGAAACTAAGACTCCTGGTGCCATGGAGCATGAACTGGTCTTGCACCAGCTCAGGTGTAAC GGGGTGCTGGAAGGCATCCGCATCTGTAGGAAGGGGTTCCCCAGCAGGATCCTCTATGCAGACTTCAAGC AGAGATACAAAGTCCTGAATGCAAGTGCCATCCCTGAGGGTCAGTTCATTGATAGCAAGAAGGCCTCTGA GAAACTTCTGGGTTCCATTGACATTGACCACACTCAGTACAAATTCGGTCACACTAAGGTTTTCTTTAAA GCCGGCCTGTTGGGAACTCTAGAGGAGATGCGAGATGAAAAGCTGGCTCAGCTCATCACCCGCACTCAAG CCGTGTGCAGAGGGTACCTGATGAGGGTGGAGTTCAAAAAGATGATGGAGAGGAGAGAGTCCATCTTCTG CATTCAGTACAACGTCCGCGCCTTCATGAATGTGAAACACTGGCCCTGGATGAAGCTGTATTTCAAGATC AAACCTCTGCTGAAGAGCGCGGAGACCGAGAAGGAGATGGCCAACATGAAGGAAGATTTTGAGAAGGCCA AGGAGGATCTGGCTAAGTCAGAGGCAAAAAGGAAGGAACTAGAAGAAAAGATGGTAGCTTTGATGCAGGA GAAAAATGACCTGCAGCTCCAGGTTCAAGCTGAAGCAGATGGCTTGGCTGATGCTGAGGAAAGGTGCGAC CAGCTGATCAAAACCAAAATCCAGCTGGAGGCCAAAATCAAAGAGCTGACCGAGAGGGCCGAGGACGAGG AGGAGATCAACGCGGAGCTCACGGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCAGAGCTGAAGAAGGA CATTGACGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACGGAGAACAAGGTGAAA AACCTGACAGAAGAGATGGCAGGCCTGGATGAAAATATCGCCAAGCTGACCAAGGAGAAGAAGGCCCTCC AGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCTGACCAAAGC CAAAACCAAGCTGGAACAGCAAGTGGATGACCTTGAAGGATCACTGGAACAGGAAAAGAAGCTGAGGATG GACTTAGAAAGAGCCAAGAGGAAACTGGAGGGGGACCTGAAACTGGCCCAAGAGTCCACAATGGACATAG AAAACGATAAACAGCAGCTCGATGAGAAACTCAAAAAGAAAGAGTTTGAAATGAGCAATCTGCAGAGCAA GATTGAGGACGAGCAGGCCCTCGGCATGCAGCTGCAGAAGAAGATCAAGGAGTTGCAGGCCCGCATCGAG GAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCAGGGCTAAAGCAGAGAAGCAGCGCTCTGACCTCT CCCGGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCTGGCGGAGCCACTTCAGCCCAGATCGAGAT GAACAAGAAGAGAGAGGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACGCTGCAGCATGAA GCCACAGCAGCCGCTCTTCGGAAGAAGCACGCGGACAGCGTGGCTGAGCTCGGGGAGCAGATCGACAACC TGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGCTGAAGATGGAGATCGATGATCTCGCTAG TAACATGGAGACAGTCTCCAAAGCCAAGGGGAACCTTGAGAAGATGTGCCGCACCCTGGAGGACCAACTG AGTGAAGTGAAGACCAAGGAGGAGGAGCAGCAGCGCCTGATCAATGAACTGTCAACCCAGAAGGCACGCT TGCACACAGAGTCAGGCGAGTTTTCCCGACAGCTGGATGAAAAAGACGCTATGGTTTCTCAGCTTTCCCG AGGCAAACAAGCATTTACACAACAGATTGAGGAACTAAAGAGGCAGCTAGAGGAAGAGTCCAAGGCCAAG AACGCACTGGCCCACGCCCTGCAGTCAGCTCGCCATGACTGTGACCTGCTGCGGGAACAGTATGAAGAGG AGCAGGAAGCCAAGGCTGAGCTGCAGAGGGCCATGTCCAAGGCCAACAGCGAGGTAGCCCAGTGGAGGAC GAAATATGAGACGGATGCCATCCAGCGCACAGAGGAGCTGGAAGAGGCCAAGAAGAAGCTGGCTCAGCGT CTGCAGGATGCTGAGGAACATGTAGAAGCTGTGAATTCCAAATGCGCTTCTCTTGAAAAGACGAAGCAGC GGCTTCAGAATGAAGTGGAGGACCTCATGATTGACGTGGAGAGGTCTAACGCTGCCTGCGCTGCGCTTGA TAAGAAGCAGAGGAACTTTGACAAGGTCCTGGCAGAATGGAAACAGAAGTACGAGGAAACTCAGGCTGAG CTTGAAGCCTCCCAGAAGGAGTCCCGCTCTCTGAGCACTGAGCTGTTCAAAGTGAAGAATGCCTATGAGG AGTCTCTGGATCAACTTGAGACGTTAAAGAGAGAGAACAAGAACCTGCAGCAGGAGATCTCTGACCTGAC CGAGCAGATTGCTGAGGGAGGGAAGCACATCCATGAGCTGGAGAAAATCAAGAAGCAAATTGATCAGGAA AAGAGTGAATTACAGGCTTCTCTGGAGGAAGCGGAGGCATCCCTTGAACATGAAGAAGGTAAAATCCTAC GCATCCAGCTGGAGTTGAACCAGGTGAAATCTGAGATTGACCGCAAAATTGCTGAAAAAGATGAAGAGAT AGATCAGCTGAAGAGAAACCACCTCAGAGTTGTGGAGTCCATGCAGAGCACGCTGGACGCCGAGATCCGC AGCAGGAATGATGCTCTGAGGATTAAGAAGAAGATGGAGGGAGACCTCAATGAGATGGAGATCCAGCTGA ACCATGCCAACCGCCAGGCTGCGGAGGCAATCAGGAACCTTCGGAACACCCAGGGAATGCTGAAGGACAC ACAGCTGCACCTGGACGATGCTCTCAGAGGCCAGGACGACCTGAAAGAGCAGCTGGCCATGGTTGAGCGC AGAGCCAACCTGATGCAGGCTGAGATCGAGGAGCTCAGGGCATCCCTGGAACAGACAGAGAGGAGCAGGA GAGTGGCCGAGCAAGAGCTACTGGATGCCAGTGAGCGCGTGCAGCTCCTCCACACCCAGAACACCAGCCT CATCAACACCAAGAAGAAGCTGGAGACAGACATTTCCCAAATCCAGGGAGAGATGGAAGACATCGTCCAG GAAGCCCGCAACGCAGAAGAGAAGGCCAAGAAAGCCATCACTGATGCCGCCATGATGGCGGAGGAGCTGA AGAAGGAGCAGGACACCAGCGCCCACCTGGAGCGGATGAAGAAGAACATGGAGCAGACCGTGAAGGACCT GCAGCACCGTCTGGACGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATCCAGAAACTGGAG GCTAGGGTGAGGGAGCTTGAAAACGAGGTGGAAAATGAACAGAAGCGCAACATCGAAGCCGTCAAGGGTC TTCGTAAGCACGAGCGCAGAGTGAAGGAACTCACCTACCAGACCGAGGAGGACCGCAAGAACGTGCTGAG GCTGCAGGACTTGGTGGACAAACTACAGACTAAAGTGAAAGCCTACAAGAGACAGGCTGAGGAGGCTGAG GAACAATCCAATGTCAACCTGGCCAAGTTCCGTAAGATCCAGCACGAGCTGGAGGAAGCCGAGGAGCGGG CTGACATCGCGGAGTCCCAGGTCAACAAGCTGCGGGTGAAGAGCCGAGAGGTTCACACTAAAGTCATAAG CGAAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226919 representing NM_010855
Red=Cloning site Green=Tags(s) MSSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVVDAKESYVKATVQSREGGKVTAKTEGGATV TVKDDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVV AAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGDKKKEEA TSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQL KAERSYHIFYQIMSNKKPELIEMLLITTNPYDFAYVSQGEITVPSIDDQEELMATDTAVDILGFSADEKV AIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLTSLNSADLLKALCYPRVKVGNEYVTKGQTV QQVYNSVGALAKSMYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNTLEQLCINFTNEKLQQ FFNHHMFVLEQEEYKKEGIDWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHL GKSNNFQKPKPAKGKAEAHFSLVHYAGTVDYNIIGWLDKNKDPLNETVVGLYQKSGLKTLAFLFSGGQAA EAEGGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLKSTHPHFVRCLIPNETKTPGAMEHELVLHQLRCN GVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKVFFK AGLLGTLEEMRDEKLAQLITRTQAVCRGYLMRVEFKKMMERRESIFCIQYNVRAFMNVKHWPWMKLYFKI KPLLKSAETEKEMANMKEDFEKAKEDLAKSEAKRKELEEKMVALMQEKNDLQLQVQAEADGLADAEERCD QLIKTKIQLEAKIKELTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVK NLTEEMAGLDENIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRM DLERAKRKLEGDLKLAQESTMDIENDKQQLDEKLKKKEFEMSNLQSKIEDEQALGMQLQKKIKELQARIE ELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHE ATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMETVSKAKGNLEKMCRTLEDQL SEVKTKEEEQQRLINELSTQKARLHTESGEFSRQLDEKDAMVSQLSRGKQAFTQQIEELKRQLEEESKAK NALAHALQSARHDCDLLREQYEEEQEAKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQR LQDAEEHVEAVNSKCASLEKTKQRLQNEVEDLMIDVERSNAACAALDKKQRNFDKVLAEWKQKYEETQAE LEASQKESRSLSTELFKVKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKHIHELEKIKKQIDQE KSELQASLEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHLRVVESMQSTLDAEIR SRNDALRIKKKMEGDLNEMEIQLNHANRQAAEAIRNLRNTQGMLKDTQLHLDDALRGQDDLKEQLAMVER RANLMQAEIEELRASLEQTERSRRVAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIVQ EARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQHRLDEAEQLALKGGKKQIQKLE ARVRELENEVENEQKRNIEAVKGLRKHERRVKELTYQTEEDRKNVLRLQDLVDKLQTKVKAYKRQAEEAE EQSNVNLAKFRKIQHELEEAEERADIAESQVNKLRVKSREVHTKVISEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_010855 |
ORF Size | 5820 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 6037 bp |
RefSeq ORF | 5820 bp |
Locus ID | 17884 |
UniProt ID | Q5SX39 |
MW | 222.9 kDa |
Gene Summary | Muscle contraction.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |