Sftpb (NM_147779) Mouse Tagged ORF Clone
CAT#: MR226883
- TrueORF®
Sftpb (Myc-DDK-tagged) - Mouse surfactant associated protein B (Sftpb)
ORF Plasmid: tGFP
"NM_147779" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI562151; SF-B; Sftp-3; Sftp3; SP-B |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226883 representing NM_147779
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAGTCGCACCTACTGCAGTGGCTACTGCTGCTTCCTACCCTCTGCTGCCCAGGTGCAGCTATCA CGTCGGCCTCATCCCTGGAGTGTGCACAAGGCCCTCAATTCTGGTGCCAAAGCCTGGAGCATGCAGTGCA GTGCAGAGCCCTGGGGCACTGCCTGCAGGAAGTCTGGGGGCATGCAGGAGCTAATGACCTGTGCCAAGAG TGTGAGGATATTGTCCACCTCCTCACAAAGATGACCAAGGAAGATGCTTTCCAGGAAGCAATCCGGAAGT TCCTGGAACAAGAATGTGATATCCTTCCCTTGAAGCTGCTTGTGCCCCGGTGTCGCCAAGTGCTTGATGT CTACCTGCCCCTGGTTATTGACTACTTCCAGAGCCAGATTAACCCCAAAGCCATCTGCAATCATGTAGGC CTGTGCCCACGTGGGCAGGCTAAGCCAGAACAGAATCCAGGGATGCCGGATGCCGTTCCAAACCCTCTGC TGGACAAGCTGGTCCTCCCTGTGCTGCCAGGAGCCCTCTTGGCAAGGCCTGGGCCTCACACTCAGGACTT CTCTGAGCAACAGCTCCCCATTCCCCTGCCCTTCTGCTGGCTTTGCAGAACTCTGATCAAGCGGGTTCAA GCCGTGATCCCCAAGGGTGTGCTGGCTGTGGCTGTGTCCCAGGTGTGCCACGTGGTACCCCTGGTGGTGG GTGGCATCTGCCAGTGCCTGGCTGAGCGCTACACAGTTCTCCTGCTAGACGCACTGCTGGGCCGTGTGGT GCCCCAGCTAGTCTGTGGCCTTGTCCTCCGATGTTCCACTGAGGATGCCATGGGCCCTGCCCTCCCTGCT GTGGAGCCTCTGATAGAAGAATGGCCACTACAGGACACTGAGTGCCATTTCTGCAAGTCTGTGATCAACC AGGCCTGGAACACCAGTGAACAGGCTATGCCACAGGCAATGCACCAGGCCTGCCTTCGCTTCTGGCTAGA CAGGCAAAAGTGTGAACAGTTTGTGGAACAGCACATGCCCCAGCTGCTGGCCCTGGTGCCTAGGAGCCAG GATGCCCACATCACCTGCCAGGCCCTTGGCGTATGTGAGGCCCCGGCTAGCCCTCTGCAGTGCTTCCAAA CCCCACACCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226883 representing NM_147779
Red=Cloning site Green=Tags(s) MAKSHLLQWLLLLPTLCCPGAAITSASSLECAQGPQFWCQSLEHAVQCRALGHCLQEVWGHAGANDLCQE CEDIVHLLTKMTKEDAFQEAIRKFLEQECDILPLKLLVPRCRQVLDVYLPLVIDYFQSQINPKAICNHVG LCPRGQAKPEQNPGMPDAVPNPLLDKLVLPVLPGALLARPGPHTQDFSEQQLPIPLPFCWLCRTLIKRVQ AVIPKGVLAVAVSQVCHVVPLVVGGICQCLAERYTVLLLDALLGRVVPQLVCGLVLRCSTEDAMGPALPA VEPLIEEWPLQDTECHFCKSVINQAWNTSEQAMPQAMHQACLRFWLDRQKCEQFVEQHMPQLLALVPRSQ DAHITCQALGVCEAPASPLQCFQTPHL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_147779 |
ORF Size | 1131 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_147779.1, NP_680088.1 |
RefSeq Size | 1887 bp |
RefSeq ORF | 1134 bp |
Locus ID | 20388 |
UniProt ID | P50405 |
MW | 57.3 kDa |
Gene Summary | Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209384 | Sftpb (untagged) - Mouse surfactant associated protein B (Sftpb), (10ug) |
CNY 3,656.00 |
|
MG226883 | Sftpb (tGFP-tagged) - Mouse surfactant associated protein B (Sftpb), (10ug) |
CNY 3,140.00 |
|
MR226883L3 | Lenti ORF clone of Sftpb (Myc-DDK-tagged) - Mouse surfactant associated protein B (Sftpb) |
CNY 4,750.00 |
|
MR226883L4 | Lenti ORF clone of Sftpb (mGFP-tagged) - Mouse surfactant associated protein B (Sftpb) |
CNY 4,750.00 |