United States
Germany
Japan
United Kingdom
China
Inppl1 (NM_001122739) Mouse Tagged ORF Clone
CAT＃: MR226853
- TrueORF®
Inppl1 (Myc-DDK-tagged) - Mouse inositol polyphosphate phosphatase-like 1 (Inppl1), transcript variant 2
ORF Plasmid: tGFP
"NM_001122739" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 9,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 51C; SHIP2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226853 representing NM_001122739
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCAGTGTGTGGGACACCGAGTCCCGGGGGTGCGCTAGGCAGCCCGGCCCCAGCCTGGTATCACC GTGACCTGAGCCGCGCTGCTGCGGAGGAGCTCCTGGCTCGGGCGGGCCGCGATGGCAGCTTCCTGGTGCG AGATAGCGAGAGCGTGGCGGGGGCCTTCGCACTCTGCGTCCTGTATCAAAAGCACGTGCACACCTACCGC ATTCTGCCAGATGGAGAGGATTTCCTGGCTGTGCAGACCTCACAGGGTGTTCCTGTGCGTCGCTTCCAGA CCCTGGGTGAGCTTATAGGCCTATATGCCCAGCCCAACCAGGGTCTTGTTTGTGCCCTGCTGCTGCCTGT AGAGGGGGAGAGAGAGCCAGACCCACCAGATGACCGAGATGCCTCAGATGTGGAGGACGAGAAGCCCCCA CTACCCCCGCGCTCTGGCTCTACCAGCATTTCTGCCCCTGTGGGGCCCAGCAGCCCCCTGCCGACTCCCG AGACTCCCACAACTCCAGCAGCTGAGAGCACTCCTAATGGACTCAGCACTGTGTCACATGAGTATCTGAA GGGCAGCTACGGGCTGGACCTGGAGGCTGTACGAGGTGGAGCCAGCAACTTGCCACATCTCACCAGAACC CTTGTCACCTCGTGCCGAAGGCTACACAGCGAGGTGGACAAGGTCCTGTCAGGCTTGGAGATCCTGTCGA AGGTGTTTGACCAGCAGAGCTCGCCCATGGTGACCCGCCTTTTACAGCAGCAGAGCTTACCACAGACTGG AGAGCAGGAGCTGGAGAGCCTTGTGCTGAAGCTATCTGTGTTAAAGGACTTCCTGTCAGGCATCCAGAAG AAGGCCCTAAAGGCACTGCAGGACATGAGCTCCACAGCGCCTCCGGCTCCATTGCAGCCCTCCATACGCA AGGCCAAGACCATCCCCGTGCAGGCCTTTGAGGTGAAGCTGGATGTAACACTGGGTGACCTGACCAAGAT TGGGAAGTCCCAGAAGTTCACGCTGAGTGTGGATGTCGAGGGTGGGAGGCTGGTTCTGCTGAGGAGACAG CGTGACTCCCAGGAGGACTGGACAACCTTCACACATGACCGGATCCGGCAGCTCATTAAATCCCAGCGTG TGCAGAACAAGCTGGGTGTTGTGTTTGAAAAGGAGAAAGATCGGACCCAGCGCAAGGACTTCATCTTTGT CAGTGCCCGGAAACGAGAAGCCTTCTGCCAGCTCCTGCAGCTCATGAAGAACAGGCACTCCAAGCAGGAC GAGCCCGACATGATCTCTGTCTTCATAGGCACCTGGAACATGGGAAGTGTACCACCACCCAAAAACGTGA CATCTTGGTTCACATCAAAGGGACTGGGGAAAGCTCTGGATGAGGTCACAGTGACTATACCCCACGATAT CTATGTCTTTGGGACTCAGGAGAACTCAGTGGGTGACAGAGAGTGGCTGGATCTGCTGCGTGGGGGCCTC AAGGAGCTTACAGATCTGGATTACCGTCCGATTGCTATGCAGTCACTATGGAACATCAAGGTGGCTGTGT TGGTCAAGCCAGAACATGAGAATCGCATCAGCCACGTCAGTACGTCCAGTGTGAAGACTGGTATCGCCAA CACCCTGGGGAACAAGGGAGCCGTGGGTGTGTCCTTCATGTTCAATGGCACCTCATTTGGCTTCGTGAAT TGCCATCTCACCTCAGGGAATGAGAAGACTACTCGGCGGAACCAGAATTATCTGGACATTCTGCGTCTCC TCTCGTTGGGTGATCGGCAGCTCAGTGCCTTTGACATCTCTTTGCGGTTCACTCATCTCTTCTGGTTTGG GGACCTTAACTACCGCTTAGACATGGATATCCAGGAGATCCTGAACTACATTAGTAGGAGAGAGTTTGAG CCTCTGCTCAGGGTGGACCAGCTCAACCTGGAGCGGGAGAAGCATAAGGTCTTCCTTCGATTTAGTGAGG AGGAGATATCTTTCCCACCCACCTACCGCTACGAGCGGGGTTCCCGGGACACATACGCTTGGCACAAGCA GAAGCCAACTGGGGTCCGGACCAATGTGCCTTCGTGGTGTGACCGGATTCTATGGAAATCCTATCCTGAA ACCCACATCATCTGCAATTCCTATGGTTGCACTGATGACATTGTTACCAGTGACCATTCTCCTGTGTTTG GGACATTTGAGGTTGGAGTTACTTCCCAGTTCATCTCCAAGAAAGGTCTCTCTAAGACCTCAGATCAAGC CTACATTGAGTTTGAGAGTATCGAAGCCATTGTGAAGACAGCCAGCCGCACCAAGTTCTTCATTGAGTTC TATTCTACCTGCTTGGAAGAGTATAAGAAGAGCTTCGAGAATGATGCTCAGAGCAGCGACAACATCAATT TCCTCAAGGTGCAGTGGTCCTCCCGCCAGCTACCCACGCTCAAGCCAATTCTGGCTGACATTGAGTACCT GCAGGATCAGCATCTCCTGCTCACAGTCAAGTCCATGGATGGCTACGAATCATATGGGGAGTGTGTGGTT GCACTCAAGTCCATGATTGGCAGCACAGCCCAGCAGTTCCTGACCTTCTTGTCCCACCGTGGAGAGGAGA CAGGCAACATCCGTGGCTCCATGAAGGTGCGGGTGCCCACAGAGCGCCTGGGCACCCGTGAGCGGCTCTA TGAATGGATTAGCATTGATAAGGATGACACAGGAGCCAAAAGCAAGGTTCCTTCAGTGTCAAGAGGCAGC CAGGAGCACAGATCTGGGAGCCGCAAGCCAGCTTCCACAGAGACCTCCTGTCCACTGTCCAAGTTGTTTG AAGAGCCTGAAAAGCCACCACCGACTGGCAGACCCCCGGCCCCACCACGGGCAGTTCCTAGGGAGGAGCC CTTGAACCCAAGGCTGAAGTCAGAAGGGACATCTGAGCAGGAAGGAGTAGCAGCCCCCCCACCCAAGAAC AGCTTCAATAACCCTGCCTACTACGTCCTTGAAGGGGTCCCACATCAGCTGCTGCCCCTGGAGCCACCCT CACTTGCCAGGGCCCCTCTCCCACCTGCCACCAAGAACAAAGTGGCCATCACAGTGCCTGCTCCTCAGCT TGGGCGCCACCGGACCCCTCGTGTGGGGGAGGGAAGCTCATCAGATGAGGACTCTGGGGGCACGCTGCCT CCTCCAGATTTCCCACCTCCACCACTGCCAGACTCAGCCATCTTCCTGCCCCCTAACCTGGATCCTTTGT CAATGCCAGTGGTCAGGGGCCGAAGTGGGGGTGAGGCCCGTGGCCCACCACCTCCCAAGGCCCATCCAAG ACCACCACTACCGCCAGGCACCTCACCTGCCAGTACTTTTTTGGGAGAGGTCGCAAGTGGGGATGACCGG TCTTGTTCGGTACTGCAAATGGCCAAAACACTCAGTGAGGTAGATTATGCTCCAGGGCCTGGACGCTCAG CACTCCTCCCCAACCCCTTGGAATTGCAGCCTCCCCGAGGGCCCTCGGACTATGGCCGGCCCCTCAGCTT CCCTCCACCCCGCATCCGGGAGAGCATCCAAGAAGACTTGGCAGAGGAGGCTCCGTGCCCGCAGGGCGGG CGGGCCAGCGGGCTGGGAGAGGCGGGCATGGGTGCCTGGCTGCGGGCCATCGGCTTGGAGCGCTATGAGG AGGGCCTGGTGCACAATGGCTGGGACGACCTGGAGTTTCTCAGTGACATCACTGAGGAAGACCTTGAGGA AGCTGGGGTGCAGGATCCTGCTCACAAGCGCCTTCTTCTGGACACCTTGCAGCTCAGCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226853 representing NM_001122739
Red=Cloning site Green=Tags(s) MASVCGTPSPGGALGSPAPAWYHRDLSRAAAEELLARAGRDGSFLVRDSESVAGAFALCVLYQKHVHTYR ILPDGEDFLAVQTSQGVPVRRFQTLGELIGLYAQPNQGLVCALLLPVEGEREPDPPDDRDASDVEDEKPP LPPRSGSTSISAPVGPSSPLPTPETPTTPAAESTPNGLSTVSHEYLKGSYGLDLEAVRGGASNLPHLTRT LVTSCRRLHSEVDKVLSGLEILSKVFDQQSSPMVTRLLQQQSLPQTGEQELESLVLKLSVLKDFLSGIQK KALKALQDMSSTAPPAPLQPSIRKAKTIPVQAFEVKLDVTLGDLTKIGKSQKFTLSVDVEGGRLVLLRRQ RDSQEDWTTFTHDRIRQLIKSQRVQNKLGVVFEKEKDRTQRKDFIFVSARKREAFCQLLQLMKNRHSKQD EPDMISVFIGTWNMGSVPPPKNVTSWFTSKGLGKALDEVTVTIPHDIYVFGTQENSVGDREWLDLLRGGL KELTDLDYRPIAMQSLWNIKVAVLVKPEHENRISHVSTSSVKTGIANTLGNKGAVGVSFMFNGTSFGFVN CHLTSGNEKTTRRNQNYLDILRLLSLGDRQLSAFDISLRFTHLFWFGDLNYRLDMDIQEILNYISRREFE PLLRVDQLNLEREKHKVFLRFSEEEISFPPTYRYERGSRDTYAWHKQKPTGVRTNVPSWCDRILWKSYPE THIICNSYGCTDDIVTSDHSPVFGTFEVGVTSQFISKKGLSKTSDQAYIEFESIEAIVKTASRTKFFIEF YSTCLEEYKKSFENDAQSSDNINFLKVQWSSRQLPTLKPILADIEYLQDQHLLLTVKSMDGYESYGECVV ALKSMIGSTAQQFLTFLSHRGEETGNIRGSMKVRVPTERLGTRERLYEWISIDKDDTGAKSKVPSVSRGS QEHRSGSRKPASTETSCPLSKLFEEPEKPPPTGRPPAPPRAVPREEPLNPRLKSEGTSEQEGVAAPPPKN SFNNPAYYVLEGVPHQLLPLEPPSLARAPLPPATKNKVAITVPAPQLGRHRTPRVGEGSSSDEDSGGTLP PPDFPPPPLPDSAIFLPPNLDPLSMPVVRGRSGGEARGPPPPKAHPRPPLPPGTSPASTFLGEVASGDDR SCSVLQMAKTLSEVDYAPGPGRSALLPNPLELQPPRGPSDYGRPLSFPPPRIRESIQEDLAEEAPCPQGG RASGLGEAGMGAWLRAIGLERYEEGLVHNGWDDLEFLSDITEEDLEEAGVQDPAHKRLLLDTLQLSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001122739 |
| ORF Size | 3771 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001122739.1, NP_001116211.1 |
| RefSeq Size | 4778 bp |
| RefSeq ORF | 3774 bp |
| Locus ID | 16332 |
| UniProt ID | Q6P549 |
| MW | 139.4 kDa |
| Gene Summary | Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways (PubMed:10958682). Plays a central role in regulation of PI3K-dependent insulin signaling, although the precise molecular mechanisms and signaling pathways remain unclear. While overexpression reduces both insulin-stimulated MAP kinase and Akt activation, its absence does not affect insulin signaling or GLUT4 trafficking. Confers resistance to dietary obesity. May act by regulating AKT2, but not AKT1, phosphorylation at the plasma membrane. Part of a signaling pathway that regulates actin cytoskeleton remodeling. Required for the maintenance and dynamic remodeling of actin structures as well as in endocytosis, having a major impact on ligand-induced EGFR internalization and degradation. Participates in regulation of cortical and submembraneous actin by hydrolyzing PtdIns(3,4,5)P3 thereby regulating membrane ruffling (By similarity). Regulates cell adhesion and cell spreading (PubMed:29749928). Required for HGF-mediated lamellipodium formation, cell scattering and spreading. Acts as a negative regulator of EPHA2 receptor endocytosis by inhibiting via PI3K-dependent Rac1 activation. Acts as a regulator of neuritogenesis by regulating PtdIns(3,4,5)P3 level and is required to form an initial protrusive pattern, and later, maintain proper neurite outgrowth. Acts as a negative regulator of the FC-gamma-RIIA receptor (FCGR2A). Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Involved in EGF signaling pathway. Upon stimulation by EGF, it is recruited by EGFR and dephosphorylates PtdIns(3,4,5)P3. Plays a negative role in regulating the PI3K-PKB pathway, possibly by inhibiting PKB activity. Down-regulates Fc-gamma-R-mediated phagocytosis in macrophages independently of INPP5D/SHIP1. In macrophages, down-regulates NF-kappa-B-dependent gene transcription by regulating macrophage colony-stimulating factor (M-CSF)-induced signaling. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6. Involved in endochondral ossification (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224086 | Inppl1 (untagged) - Mouse inositol polyphosphate phosphatase-like 1 (Inppl1), transcript variant 2, (10ug) |
CNY 16,250.00 |
|
| MG226853 | Inppl1 (tGFP-tagged) - Mouse inositol polyphosphate phosphatase-like 1 (Inppl1) transcript variant 2, (10ug) |
CNY 10,550.00 |
|
| MR226853L3 | Lenti ORF clone of Inppl1 (Myc-DDK-tagged) - Mouse inositol polyphosphate phosphatase-like 1 (Inppl1), transcript variant 2 |
CNY 11,500.00 |
|
| MR226853L4 | Lenti ORF clone of Inppl1 (mGFP-tagged) - Mouse inositol polyphosphate phosphatase-like 1 (Inppl1), transcript variant 2 |
CNY 11,500.00 |
