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Mapk8ip1 (NM_011162) Mouse Tagged ORF Clone
CAT＃: MR226852
- TrueORF®
Mapk8ip1 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase 8 interacting protein 1 (Mapk8ip1), transcript variant 1
ORF Plasmid: tGFP
"NM_011162" in other vectors (4)
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CNY 5,176.00
CNY 6,270.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | IB1; JIP-1; Jip1; mjip-2a; Prkm8ip; Skip |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226852 representing NM_011162
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCGAGAGAGCGGCCTGGGCGGGGGCGCCGCGTCCCCACCGGCCGCTTCCCCATTCCTGGGAC TGCACATCGCGTCGCCTCCCAATTTCAGGCTCACCCATGACATCAGCCTGGAGGAGTTTGAGGATGAAGA CCTTTCGGAGATCACTGACGAGTGTGGCATCAGCCTGCAGTGCAAAGACACCCTGTCTCTCCGGCCCCCG CGCGCCGGGCTGCTGTCTGCGGGTAGCAGCGGCAGCGCGGGGAGCCGGCTGCAGGCGGAGATGCTGCAGA TGGACCTGATCGACGCGGCAGGTGACACTCCGGGCGCCGAGGACGACGAGGAGGAGGAGGACGACGAGCT CGCTGCCCAACGACCAGGAGTGGGGCCTCCCAAAGCGGAGTCCAACCAGGATCCGGCGCCTCGCAGCCAG GGCCAGGGCCCGGGCACAGGCAGCGGAGACACCTACCGACCCAAGAGGCCTACCACGCTCAACCTTTTCC CGCAGGTGCCGCGGTCTCAGGACACGCTGAATAATAACTCTTTAGGCAAAAAGCACAGTTGGCAGGACCG TGTGTCTCGATCATCCTCCCCTCTGAAGACAGGAGAACAGACGCCTCCACATGAACACATCTGCCTGAGT GATGAGCTGCCACCCCAGGGCAGTCCTGTTCCCACCCAGGACCGCGGCACTTCCACCGACAGCCCTTGTC GCCGAAGTGCAGCCACCCAGATGGCACCTCCAAGCGGTCCCCCTGCCACTGCGCCTGGTGGCCGGGGCCA CTCCCATCGAGACCGAATCCACTACCAGGCAGATGTGCGGCTCGAGGCGACTGAGGAGATCTACCTGACC CCAGTGCAGAGGCCCCCAGACCCTGCAGAACCCACCTCCACCTTCATGCCACCCACGGAGAGCCGGATGT CAGTTAGCTCCGATCCAGACCCTGCCGCTTACTCTGTAACTGCGGGGCGGCCACACCCCTCCATCAGTGA AGAGGATGAGGGCTTCGACTGCCTGTCATCCCCAGAGCGAGCTGAGCCACCAGGTGGAGGGTGGCGGGGA AGCCTCGGGGAGCCACCACCGCCTCCACGGGCCTCACTGAGCTCGGACACCAGCGCACTGTCCTACGACT CGGTCAAGTACACACTGGTGGTGGATGAACATGCCCAGCTTGAGTTGGTGAGCCTGCGGCCGTGCTTTGG AGATTACAGTGACGAAAGCGACTCTGCCACTGTCTATGACAACTGTGCCTCTGCCTCCTCGCCCTACGAG TCAGCCATTGGTGAGGAGTATGAGGAGGCCCCTCAGCCCCGGCCTCCCACCTGCCTCTCAGAGGACTCCA CCCCGGATGAGCCTGATGTCCACTTCTCTAAGAAGTTTCTGAATGTCTTCATGAGTGGCCGCTCTCGTTC CTCCAGTGCTGAGTCCTTTGGGCTGTTCTCCTGCGTCATCAATGGGGAGGAGCATGAGCAAACCCATCGG GCTATATTCAGGTTTGTGCCTCGGCATGAAGATGAACTTGAGCTGGAAGTGGATGACCCCCTGCTGGTGG AGCTGCAGGCAGAAGACTATTGGTATGAGGCCTATAACATGCGCACCGGAGCCCGCGGGGTCTTCCCTGC CTACTATGCCATTGAGGTCACCAAGGAGCCTGAGCACATGGCAGCCCTTGCCAAAAACAGCGACTGGATT GACCAGTTCCGGGTGAAGTTCCTGGGGTCTGTCCAGGTTCCTTATCACAAGGGCAATGATGTCCTCTGTG CTGCTATGCAAAAGATCGCCACCACCCGCCGGCTCACCGTGCACTTTAACCCGCCCTCCAGCTGTGTCCT TGAGATCAGTGTCAGGGGTGTCAAGATAGGCGTCAAAGCTGATGATGCTCTGGAGGCCAAGGGAAATAAA TGTAGCCACTTCTTCCAGCTAAAGAACATCTCTTTCTGTGGATACCATCCAAAGAATAACAAGTACTTTG GGTTTATCACTAAGCACCCTGCTGACCACCGGTTTGCCTGCCATGTCTTTGTGTCTGAAGATTCCACCAA AGCCCTGGCGGAGTCTGTGGGGCGTGCATTTCAGCAGTTCTACAAGCAGTTTGTGGAGTATACCTGTCCT ACAGAAGATATCTACTTGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226852 representing NM_011162
Red=Cloning site Green=Tags(s) MAERESGLGGGAASPPAASPFLGLHIASPPNFRLTHDISLEEFEDEDLSEITDECGISLQCKDTLSLRPP RAGLLSAGSSGSAGSRLQAEMLQMDLIDAAGDTPGAEDDEEEEDDELAAQRPGVGPPKAESNQDPAPRSQ GQGPGTGSGDTYRPKRPTTLNLFPQVPRSQDTLNNNSLGKKHSWQDRVSRSSSPLKTGEQTPPHEHICLS DELPPQGSPVPTQDRGTSTDSPCRRSAATQMAPPSGPPATAPGGRGHSHRDRIHYQADVRLEATEEIYLT PVQRPPDPAEPTSTFMPPTESRMSVSSDPDPAAYSVTAGRPHPSISEEDEGFDCLSSPERAEPPGGGWRG SLGEPPPPPRASLSSDTSALSYDSVKYTLVVDEHAQLELVSLRPCFGDYSDESDSATVYDNCASASSPYE SAIGEEYEEAPQPRPPTCLSEDSTPDEPDVHFSKKFLNVFMSGRSRSSSAESFGLFSCVINGEEHEQTHR AIFRFVPRHEDELELEVDDPLLVELQAEDYWYEAYNMRTGARGVFPAYYAIEVTKEPEHMAALAKNSDWI DQFRVKFLGSVQVPYHKGNDVLCAAMQKIATTRRLTVHFNPPSSCVLEISVRGVKIGVKADDALEAKGNK CSHFFQLKNISFCGYHPKNNKYFGFITKHPADHRFACHVFVSEDSTKALAESVGRAFQQFYKQFVEYTCP TEDIYLE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011162 |
| ORF Size | 2121 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011162.5, NP_035292.2 |
| RefSeq Size | 2954 bp |
| RefSeq ORF | 2124 bp |
| Locus ID | 19099 |
| UniProt ID | Q9WVI9 |
| MW | 77.3 kDa |
| Gene Summary | The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Required for JNK activation in response to excitotoxic stress. Cytoplasmic MAPK8IP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and thus inhibiting the JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response (By similarity). Acts as a scaffold protein that coordinates with SH3RF1 in organizing different components of the JNK pathway, including RAC1 or RAC2, MAP3K11/MLK3 or MAP3K7/TAK1, MAP2K7/MKK7, MAPK8/JNK1 and/or MAPK9/JNK2 into a functional multiprotein complex to ensure the effective activation of the JNK signaling pathway. Regulates the activation of MAPK8/JNK1 and differentiation of CD8(+) T-cells (PubMed:23963642).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220748 | Mapk8ip1 (untagged) - Mouse mitogen-activated protein kinase 8 interacting protein 1 (Mapk8ip1), transcript variant 1, (10ug) |
CNY 9,120.00 |
|
| MG226852 | Mapk8ip1 (tGFP-tagged) - Mouse mitogen-activated protein kinase 8 interacting protein 1 (Mapk8ip1), (10ug) |
CNY 6,940.00 |
|
| MR226852L3 | Lenti ORF clone of Mapk8ip1 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase 8 interacting protein 1 (Mapk8ip1), transcript variant 1 |
CNY 8,170.00 |
|
| MR226852L4 | Lenti ORF clone of Mapk8ip1 (mGFP-tagged) - Mouse mitogen-activated protein kinase 8 interacting protein 1 (Mapk8ip1), transcript variant 1 |
CNY 8,170.00 |
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