United States
Germany
Japan
United Kingdom
China
Smarca4 (NM_011417) Mouse Tagged ORF Clone
CAT＃: MR226817
- TrueORF®
Smarca4 (Myc-DDK-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4), transcript variant 2
ORF Plasmid: tGFP
"NM_011417" in other vectors (5)
Need custom modification / cloning service?
Get a free quote
CNY 16,060.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | b2b508.1Clo; b2b692Clo; Brg1; HP1-BP72; SNF2beta; SW1/SNF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226817 representing NM_011417
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTACTCCAGACCCACCCTTGGGTGGGACTCCTCGGCCTGGTCCTTCCCCAGGCCCTGGTCCTTCAC CTGGTGCAATGCTGGGTCCTAGCCCTGGCCCCTCACCAGGTTCTGCCCACAGCATGATGGGGCCAAGCCC AGGACCTCCTTCAGCAGGACATCCCATGCCCACCCAGGGGCCTGGAGGGTACCCCCAGGACAACATGCAT CAGATGCACAAGCCTATGGAGTCCATGCACGAGAAGGGCATGCCTGATGACCCACGATACAACCAGATGA AAGGGATGGGCATGCGGTCAGGGGCCCACACAGGCATGGCACCTCCACCTAGTCCCATGGACCAGCATTC TCAAGGTTACCCCTCACCCCTCGGCGGCTCTGAACATGCCTCCAGTCCTGTCCCAGCCAGTGGCCCATCT TCAGGCCCCCAGATGTCCTCTGGGCCAGGAGGGGCCCCACTAGATGGTTCTGATCCCCAGGCCTTGGGAC AGCAAAACAGAGGCCCAACCCCATTTAACCAGAACCAGCTGCATCAACTCAGAGCTCAGATAATGGCCTA CAAGATGTTGGCCAGGGGCCAGCCATTGCCCGACCACCTGCAGATGGCCGTGCAAGGCAAGCGGCCGATG CCTGGAATGCAGCAACAGATGCCAACACTACCTCCACCCTCAGTGTCCGCCACAGGACCCGGACCTGGAC CCGGCCCTGGCCCTGGCCCTGGCCCAGGACCAGCCCCTCCAAATTACAGTAGACCCCATGGTATGGGAGG GCCCAACATGCCTCCCCCAGGACCCTCAGGTGTGCCCCCCGGGATGCCTGGTCAGCCGCCTGGAGGGCCT CCCAAGCCATGGCCTGAAGGACCCATGGCCAATGCTGCTGCCCCCACAAGCACCCCACAGAAGCTGATTC CTCCGCAACCAACAGGCCGTCCTTCACCTGCACCTCCTGCTGTCCCGCCTGCTGCCTCACCTGTAATGCC ACCACAAACACAGTCCCCAGGGCAGCCAGCCCAGCCTGCTCCATTGGTGCCACTGCACCAGAAGCAGAGC CGAATCACCCCCATCCAGAAGCCCCGAGGCCTTGACCCTGTGGAGATCCTACAAGAGCGGGAGTACAGGC TTCAGGCTCGAATCGCACACAGAATTCAGGAACTTGAAAACCTCCCTGGGTCCCTGGCTGGGGACCTTCG AACCAAAGCAACCATCGAACTCAAGGCCCTTAGGTTGCTGAACTTCCAGAGGCAGCTGCGCCAGGAGGTG GTGGTGTGCATGCGAAGAGACACAGCCCTGGAGACAGCCCTCAATGCCAAGGCCTACAAGCGCAGCAAAC GTCAGTCACTACGGGAGGCCCGCATCACTGAGAAGTTGGAGAAGCAGCAGAAGATTGAACAGGAGCGCAA GCGCCGCCAGAAGCACCAGGAGTACCTCAACAGCATTCTGCAGCATGCCAAGGACTTCAGGGAGTATCAC AGATCAGTCACAGGCAAACTCCAGAAACTCACCAAGGCTGTGGCCACCTACCATGCCAACACTGAGCGGG AGCAGAAGAAAGAAAATGAGCGCATTGAGAAGGAGCGAATGCGGAGGCTTATGGCTGAAGATGAGGAGGG CTACCGCAAACTCATTGACCAGAAGAAGGACAAGCGCCTGGCCTACCTTCTGCAGCAGACAGATGAGTAT GTGGCCAACCTCACAGAGCTGGTGCGGCAGCACAAAGCTGCCCAGGTTGCCAAGGAGAAGAAGAAGAAAA AGAAAAAGAAGAAGGCAGAAAATGCTGAAGGACAGACACCTGCTATTGGACCAGATGGTGAGCCTCTGGA TGAGACCAGCCAGATGAGTGACCTCCCTGTGAAGGTGATCCACGTGGAGAGTGGCAAGATCCTCACTGGC ACAGATGCCCCAAAAGCCGGGCAGCTGGAAGCCTGGCTTGAAATGAACCCAGGGTATGAAGTAGCCCCCA GGTCAGACAGTGAAGAAAGTGGCTCTGAAGAGGAGGAGGAGGAGGAGGAAGAGGAGCAGCCTCAGCCCGC ACAGCCCCCTACACTGCCTGTGGAAGAAAAGAAGAAGATTCCAGACCCAGACAGCGATGATGTCTCTGAG GTGGACGCCCGACACATTATTGAGAACGCCAAGCAAGATGTGGACGATGAGTACGGTGTGTCCCAGGCCC TTGCTCGTGGCCTGCAGTCTTACTATGCTGTGGCCCATGCAGTCACAGAGAGAGTAGATAAGCAGTCCGC CCTCATGGTCAACGGTGTCCTCAAACAGTACCAGATCAAGGGTTTGGAGTGGCTGGTGTCCCTGTACAAC AACAACCTGAATGGCATCCTGGCTGATGAGATGGGGCTGGGGAAGACCATCCAGACCATCGCGCTCATCA CATACCTCATGGAGCACAAGCGCATCAACGGGCCTTTCCTCATCATCGTGCCTCTCTCGACACTGTCAAA CTGGGCGTATGAATTTGACAAGTGGGCCCCCTCTGTGGTGAAGGTTTCTTACAAGGGCTCTCCAGCTGCA AGGCGAGCTTTTGTCCCACAGCTTCGCAGTGGGAAGTTCAACGTCTTACTGACCACCTATGAATATATCA TCAAAGACAAGCATATCCTAGCCAAGATCCGCTGGAAGTACATGATTGTGGATGAAGGCCACCGCATGAA AAACCACCACTGCAAGTTGACGCAGGTCCTTAACACACACTACGTGGCCCCTCGGCGCCTGCTTCTTACA GGCACACCACTGCAGAACAAGCTACCGGAGCTCTGGGCCCTGCTTAACTTCCTGCTCCCCACTATCTTCA AGAGCTGCAGCACCTTCGAACAGTGGTTCAATGCACCCTTTGCCATGACTGGAGAAAAGGTGGACCTGAA TGAAGAGGAGACTATCCTCATTATTCGTCGCCTACACAAAGTTCTGCGGCCCTTCCTGCTGCGGCGGCTC AAGAAGGAAGTTGAAGCCCAGCTCCCTGAGAAGGTAGAGTATGTCATCAAATGCGACATGTCAGCCCTGC AGCGTGTGCTGTACCGTCACATGCAGGCCAAAGGTGTGCTGCTGACTGACGGCTCCGAGAAGGACAAGAA GGGCAAAGGTGGCACCAAGACACTGATGAACACTATTATGCAACTGCGTAAGATCTGCAACCACCCCTAC ATGTTCCAGCACATCGAGGAGTCCTTTTCTGAGCACTTGGGGTTCACCGGCGGCATCGTGCAAGGATTGG ACCTTTACCGTGCCTCAGGGAAATTTGAACTTCTTGATAGAATTCTACCCAAACTCCGTGCAACGAACCA TAAAGTGCTCCTCTTTTGCCAAATGACCTCCCTCATGACCATCATGGAAGACTACTTTGCATACCGTGGC TTCAAATACCTCAGGCTTGATGGAACCACAAAAGCAGAAGACCGGGGCATGCTGTTGAAAACCTTTAATG AACCTGGCTCTGAGTATTTCATTTTCCTGCTCAGTACCCGTGCTGGGGGGCTGGGCCTGAATCTGCAGTC AGCTGACACTGTGATCATCTTTGACAGTGACTGGAATCCCCACCAGGACCTGCAAGCACAGGATCGAGCC CATCGCATTGGACAGCAGAATGAGGTGCGTGTTCTTCGCCTGTGCACGGTCAACAGTGTGGAAGAGAAGA TACTGGCTGCTGCCAAATACAAACTCAATGTGGATCAGAAGGTGATCCAGGCAGGCATGTTCGACCAGAA GTCGTCCAGCCATGAGAGGCGTGCCTTCCTGCAGGCCATCCTGGAGCACGAGGAGCAGGATGAGGAGGAA GATGAGGTGCCTGATGATGAGACCGTCAACCAGATGATTGCCCGGCACGAAGAAGAGTTTGACCTCTTCA TGCGCATGGACTTGGACCGCCGGCGTGAAGAAGCCCGCAACCCCAAGCGGAAGCCACGCCTGATGGAAGA GGATGAGCTCCCATCCTGGATCATCAAGGATGATGCCGAGGTGGAGCGGCTGACATGTGAAGAGGAAGAG GAGAAGATGTTCGGCCGTGGTTCTCGCCACCGCAAGGAGGTAGACTACAGCGACTCACTGACAGAGAAGC AGTGGCTCAAGGCTATCGAGGAGGGCACGCTGGAGGAGATCGAAGAGGAGGTCCGGCAGAAGAAATCTTC ACGTAAGCGTAAGCGAGACAGCGAGGCCGGCTCCTCCACCCCGACCACCAGCACCCGCAGCCGTGACAAG GATGAGGAGAGCAAGAAGCAGAAGAAACGTGGGCGGCCACCTGCTGAGAAGCTGTCCCCAAACCCACCTA ACCTCACCAAGAAGATGAAGAAGATCGTGGATGCTGTGATCAAGTACAAAGACAGCAGCAGTGGACGTCA GCTCAGCGAGGTGTTCATCCAGCTCCCCTCTCGCAAGGAGCTTCCTGAGTACTATGAGCTCATCCGAAAG CCTGTGGACTTCAAGAAGATCAAGGAACGCATCCGAAACCACAAGTACCGCAGCCTCAATGACCTGGAGA AGGATGTGATGCTGCTGTGCCAGAACGCTCAGACGTTCAACCTCGAGGGTTCCCTGATCTATGAGGACTC CATCGTCCTGCAGTCTGTCTTCACCAGCGTACGGCAGAAGATTGAGAAGGAGGACGACAGTGAAGGCGAG GAAAGCGAGGAGGAGGAGGAGGGCGAGGAGGAAGGCTCCGAGTCTGAGTCCCGCTCCGTCAAGGTGAAGA TCAAGCTGGGCCGCAAGGAGAAGGCCCAGGACCGACTCAAGGGGGGCCGCCGGCGGCCAAGCCGGGGATC CCGGGCCAAGCCGGTTGTGAGTGACGATGACAGTGAGGAGGAGCAGGAGGAGGACCGCTCAGGAAGTGGC AGTGAGGAAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226817 representing NM_011417
Red=Cloning site Green=Tags(s) MSTPDPPLGGTPRPGPSPGPGPSPGAMLGPSPGPSPGSAHSMMGPSPGPPSAGHPMPTQGPGGYPQDNMH QMHKPMESMHEKGMPDDPRYNQMKGMGMRSGAHTGMAPPPSPMDQHSQGYPSPLGGSEHASSPVPASGPS SGPQMSSGPGGAPLDGSDPQALGQQNRGPTPFNQNQLHQLRAQIMAYKMLARGQPLPDHLQMAVQGKRPM PGMQQQMPTLPPPSVSATGPGPGPGPGPGPGPGPAPPNYSRPHGMGGPNMPPPGPSGVPPGMPGQPPGGP PKPWPEGPMANAAAPTSTPQKLIPPQPTGRPSPAPPAVPPAASPVMPPQTQSPGQPAQPAPLVPLHQKQS RITPIQKPRGLDPVEILQEREYRLQARIAHRIQELENLPGSLAGDLRTKATIELKALRLLNFQRQLRQEV VVCMRRDTALETALNAKAYKRSKRQSLREARITEKLEKQQKIEQERKRRQKHQEYLNSILQHAKDFREYH RSVTGKLQKLTKAVATYHANTEREQKKENERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAYLLQQTDEY VANLTELVRQHKAAQVAKEKKKKKKKKKAENAEGQTPAIGPDGEPLDETSQMSDLPVKVIHVESGKILTG TDAPKAGQLEAWLEMNPGYEVAPRSDSEESGSEEEEEEEEEEQPQPAQPPTLPVEEKKKIPDPDSDDVSE VDARHIIENAKQDVDDEYGVSQALARGLQSYYAVAHAVTERVDKQSALMVNGVLKQYQIKGLEWLVSLYN NNLNGILADEMGLGKTIQTIALITYLMEHKRINGPFLIIVPLSTLSNWAYEFDKWAPSVVKVSYKGSPAA RRAFVPQLRSGKFNVLLTTYEYIIKDKHILAKIRWKYMIVDEGHRMKNHHCKLTQVLNTHYVAPRRLLLT GTPLQNKLPELWALLNFLLPTIFKSCSTFEQWFNAPFAMTGEKVDLNEEETILIIRRLHKVLRPFLLRRL KKEVEAQLPEKVEYVIKCDMSALQRVLYRHMQAKGVLLTDGSEKDKKGKGGTKTLMNTIMQLRKICNHPY MFQHIEESFSEHLGFTGGIVQGLDLYRASGKFELLDRILPKLRATNHKVLLFCQMTSLMTIMEDYFAYRG FKYLRLDGTTKAEDRGMLLKTFNEPGSEYFIFLLSTRAGGLGLNLQSADTVIIFDSDWNPHQDLQAQDRA HRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQKSSSHERRAFLQAILEHEEQDEEE DEVPDDETVNQMIARHEEEFDLFMRMDLDRRREEARNPKRKPRLMEEDELPSWIIKDDAEVERLTCEEEE EKMFGRGSRHRKEVDYSDSLTEKQWLKAIEEGTLEEIEEEVRQKKSSRKRKRDSEAGSSTPTTSTRSRDK DEESKKQKKRGRPPAEKLSPNPPNLTKKMKKIVDAVIKYKDSSSGRQLSEVFIQLPSRKELPEYYELIRK PVDFKKIKERIRNHKYRSLNDLEKDVMLLCQNAQTFNLEGSLIYEDSIVLQSVFTSVRQKIEKEDDSEGE ESEEEEEGEEEGSESESRSVKVKIKLGRKEKAQDRLKGGRRRPSRGSRAKPVVSDDDSEEEQEEDRSGSG SEED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011417 |
| ORF Size | 4842 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011417.3, NP_035547.2 |
| RefSeq Size | 6367 bp |
| RefSeq ORF | 4845 bp |
| Locus ID | 20586 |
| UniProt ID | Q3TKT4 |
| MW | 182 kDa |
| Gene Summary | Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating the calcium-dependent release of a repressor complex and the recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by SMARCA4-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves the release of HDAC1 and recruitment of CREBBP (By similarity). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development, a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role in regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1 (By similarity). Binds via DLX1 to enhancers located in the intergenic region between DLX5 and DLX6 and this binding is stabilized by the long non-coding RNA (lncRNA) Evf2 (PubMed:26138476). Binds to RNA in a promiscuous manner (PubMed:26138476). Binding to RNAs including lncRNA Evf2 leads to inhibition of SMARCA4 ATPase and chromatin remodeling activities (PubMed:26138476).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224755 | Smarca4 (untagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4), transcript variant 2, (10ug) |
CNY 20,710.00 |
|
| MG212055 | Smarca4 (tGFP-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4) |
CNY 13,300.00 |
|
| MG226817 | Smarca4 (tGFP-tagged) - Mouse SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 4 (Smarca4) transcript variant 2, (10ug) |
CNY 17,670.00 |
|
| MR226817L3 | Lenti ORF clone of Smarca4 (Myc-DDK-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4), transcript variant 2 |
CNY 17,960.00 |
|
| MR226817L4 | Lenti ORF clone of Smarca4 (mGFP-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4), transcript variant 2 |
CNY 17,960.00 |
其它Smarca4产品
