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Col17a1 (NM_007732) Mouse Tagged ORF Clone
CAT＃: MR226746
- TrueORF®
Col17a1 (Myc-DDK-tagged) - Mouse collagen, type XVII, alpha 1 (Col17a1)
ORF Plasmid: tGFP
"NM_007732" in other vectors (4)
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CNY 9,808.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BP180; Bpag; Bpag2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226746 representing NM_007732
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGTGACCAAGAAAAGCAAGCGAGATGGCACTGAAGTCACCGAGAGAATTGTCACGGAAACAGTAA CGACAAGACTTACATCCCTACCGCCAAAAGGGAGCACCAGCAATGGATATGCTAAGACAGGCTCTCTGGG TGGAGGAAGTCGGTTGGAGAAGCAGAGCTTGACCCATGGCAGCAGCGGCTACATCAACTCAAGTGGAAGC ATCCGAGGCAACGCTTCCACCTCCAGTTACAGGAGAACGCACTCACCGGCCTCTACCCTGCCCAACTCTC CCGGCTCCACCTTTGAAAGGAAAGCTCACATGACCCGCCATGGAACTTATGAAGGGAGCTCTAGCGGCAA CTCCTCCCCTGAGTACCCACGAAAGGAACTCGCCTCTTCTTCAACCAGAGGACGAAGCCAAACACGAGAG AGCGAAATTCGAGTTCGGTTGCAGAGCGCGTCGCCATCCACCAGATGGACAGAACTGGATGAGGTGAAGC GTTTGCTTAAGGGGAGCCGCTCTGCAAGTGCAAGTCCCACCAGGAACACCTCCAACACACTCCCCATCCC CAAGAAAGGCACCGTAGAGACCAAAACAGTGACAGCGAGCTCCCACTCAGTGTCCGGAACCTATGATTCA GCAATACTGGACACCAACTTTCCGCCCCACATGTGGTCCTCCACCTTGCCTGCAGGGTCCTCCCTGGGGA CCTATCAGAACAACATAACAGCCCAGAGCACATCCCTCCTCAACACCAATGCCTACTCAACGGGATCAGT CTTTGGAGTGCCAAATAACATGGCGTCCTGCTCTCCCACCCTGCACCCCGGACTCAGCAGCTGCTCCTCA GTGTTTGGCATGCAGAACAATCTGGCCCCCAGCTCTTCTGTCCTCTCCCATGGCACAACCACCGCTTCCA CAGCATACGGAGCGAAGAAAAACGTGCCCCAGCCTCCCACTGTCACCAGCACCGGCGTGTCCACCTCTGC CACCTGCACCACCAGTGTCCAGAGTGACGACCTCTTGCATAAAGACTGCAAGTTCCTGATTCTGGAGAAA GACAACACACCAGCTAAGAAAGAGATGGAACTCTTGATCATGACCAAGGACAGTGGGAAGGTCTTCACTG CCTCCCCTGCCACCATCTCTTCAACTTCCTTTTCAGAAGACACCCTGAAAAAGGAGAAGCAGGCTGCATA TGCCGCTGACACCTGCCTGAAGGCAGATGTGAATGGAGACCTAAATACCGTGTCCACAAAGAGCAAGATG ACCTCGGCAGAAAACCATGGCTACGACCGAGGTGGCGGTGGTGGCAGAGGCAAAGGCGGAGGTGCTGGTG GTGGCGGTGGTGGCGGTGGCGCCAGTGGCGGTGGAGGAGCATGGGGGGCTGCACCAGCCTGGTGCCCCTG CGGCTCCTGCTGCAGCTGGTGGAAGTGGCTGCTGGGCCTGCTGCTCACCTGGCTGCTGCTGCTGGGTCTG CTCTTCGGCCTCATTGCTCTGGCGGAGGAGGTAAGAAAGCTGAAGGCCCGCGTGGAGGAGCTGGAAAAGA CCAAGGTGCTATATCATGACGTCCAGATGGACAAAAGCAACAGGGACCGCCTCCAGGCCGAGGCACCCAG CCTGGGACCTGGATTAGGCAAGGCTGAGCTGGACGGCTACAGCCAGGAGGCCATCTGGCTGTTTGTAAGG AACAAGCTGATGACCGAGCAGGAGAACGGGAATCTCAGAGGAAGTCCTGGTCCAAAAGGTGACATGGGGA GTCAAGGACCTAAAGGAGACCGAGGCCTTCCTGGGACCCCAGGTATCCCTGGGCCCCTGGGCCACCCTGG CCCGGAAGGACCAAAGGGACAAAAAGGCAGCATTGGAGATCCTGGCATGGAAGGACCCATAGGCCAGAGA GGACTAGCAGGCCCCATGGGACCTCGTGGTGAACCCGGGCCTCCTGGGTCTGGAGAGAAAGGAGACAGAG GGATTGCTGGTGAACAAGGTCCTCAGGGCCTTCCTGGTGTCCCAGGTCCTCCGGGTCTCAGAGGTCACAG CGGCTCTCCTGGTCCCCAAGGCCCCCCAGGTGCTGTGGGTCCCCAAGGGCTCCGAGGTGACGTGGGACTT CCTGGTGTCAAAGGTGACAAAGGACTCATGGGACCACCAGGACCCAAAGGTGACCAGGGTGAGAAGGGAC CCAGAGGCCTCACAGGGGAGCCTGGCATTCGAGGTTTGCCTGGAGCTGTGGGTGAACCCGGAGCCAAAGG CGCAATGGGTCCGGCTGGCGCTGATGGACAGCAAGGTTCCAGAGGTGAACAAGGCTTGACAGGGATGCCT GGAACCCGGGGCCCCCCAGGACCCGCTGGAGACCCAGGAAAGCCAGGTCTCACAGGACCCCAGGGACCTC AGGGACTTCCTGGTAGCCCTGGCCGACCAGGGACTAAAGGCGAACCCGGCGCTCCCGGCAGAGTCATGAC TTCAGAGGGATCATCAACAATCACTGTGCCCGGACCTCCCGGACCTCCTGGTGCCATGGGTCCCCCAGGA CCTCCAGGGACGCCAGGTCCAGCTGGCCCTGCTGGTCTCCCAGGACAACAAGGCCCACGAGGGGAGCCAG GACTTGCTGGTGACTCATTCCTAAGCAGTGGCAGCTCCATCTCTGAGGTCCTCTCTGCCCAAGGTGTTGA CTTACGAGGTCCCCCTGGCCCACCTGGCCCACGAGGGCCACCAGGGCCTTCCATCCCAGGCCCGCCAGGA CCCAGAGGTCCACCAGGGGAAGGCGTACCAGGCCCACCCGGGCCACCAGGATCCTTCCTGACTGACTCAG AAACCTTCTTCACTGGCCCTCCAGGTCCACCCGGCCCCCCAGGTCCCAAGGGAGACCAAGGTGATCCTGG TGTCCCAGGTACTCCTGGCATCTCTGGTGGTCTTTCTCATGGGGCGTCATCAAGCACTCTGTACATGCAG GGCCCCCCTGGCCCTCCAGGGCCCCCTGGGCCTCCGGGCTCCCTCAGCAGCTCCGGCCAGGATATCCAGC ACTACATCGCTGAATACATGCAAAGTGACAACATCAGGACTTATCTCTCAGGGGTTCAGGGTCCCCCAGG CCCACCAGGTCCCCCAGGGCCTGTCATCACCATCACAGGAGAGACTTTCGACTACTCCCAGCTGGCAAGC CAGGTCGTAAGCTACTTGCGGTCATCAGGCTACGGTGCTGGCTTGTCCTCTGCCTCCTCCTCAGAAGATA TCTTGGCCATGCTGCGACGGAACGATGTGTGGCAGTACTTACGTCAGAATCTGGTGGGTCCTCCCGGTCC CCCAGGGCCACCAGGCGTCAGTGGAGATGGGTCCCTCCTGTCTTTGGACTATGGAGAGCTGAGCAGACAC ATTCTCAACTATATGTCAAGTTCTGGGATCAGCTTTGGGCATCCTGGCCCACCGGGACCCCCTGGCCTGC CAGGAACATCCTACGAGGAGCTACTGACCATGCTCCGAGCCGCTGGTTTGTCCTCCATCCCAGGCCCTCC AGGCCCACCAGGGCCCCCAGGTCCTCGAGGGCCCCCAGGTGTCTCAGCAGCTCTGTCCACCTATGCAGCT GAGAACAGCGACAACTTCCGCAGCGAGCTGATTAGCTACCTCACAAGTCCTGATGTTCGCAGCTTCATCG TTGGCCCTCCAGGCCCTCCGGGGCCACAGGGACCACCTGGAGATGGTCACTTAAGGGAGAACTACAACTG GAGCAGCAACTCCTCAGCCAGAAGGGGCACTTCCTACAGCTCTTCCACGGGCACAGGGGGAACCAACGGA GGCTCCCTGGGTGAAGGTGGAGCCTATGGTGCAGGAGATGGGGGCCCCTATGGTACCGACATCGGCCCAG GCGGAGGCTATGGGGCAGCTGCAGGTGGTGGTATATATGGCACCAATGGTGACTCGTTCCGGGATGGCTT CACTGGAGACCTAGATTACAACAAGCTGGCAGTGCGGGTGTCGGAGAGCATGCAGCGTCAGGGTCTGCTA CAAGGGATGGCCTACACTGTCCAGGGCCCACCTGGGCCCCAAGGCCCTCCTGGCATCAGCAGGGTCTTCT CAGCCTACAGCAACGTGACACAAGACCTCATGGACTTCTTCCAAACCTACGGCACTATCCCGGGACCACC TGGGCAGAAGGGAGATGTGGGAACCCCAGGCCCCAAAGGTGACAGGGGCCCTGCTGGACCACGAGGTCCT CCAGGGCCACCCGGCCCCAGAGGGAACAAAGGAGAGAAAGGAGACAAAGGTGACCAAGTCTATACTGGGA GAAGGAAGAGAAGTATTGCCATCAAGCCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226746 representing NM_007732
Red=Cloning site Green=Tags(s) MDVTKKSKRDGTEVTERIVTETVTTRLTSLPPKGSTSNGYAKTGSLGGGSRLEKQSLTHGSSGYINSSGS IRGNASTSSYRRTHSPASTLPNSPGSTFERKAHMTRHGTYEGSSSGNSSPEYPRKELASSSTRGRSQTRE SEIRVRLQSASPSTRWTELDEVKRLLKGSRSASASPTRNTSNTLPIPKKGTVETKTVTASSHSVSGTYDS AILDTNFPPHMWSSTLPAGSSLGTYQNNITAQSTSLLNTNAYSTGSVFGVPNNMASCSPTLHPGLSSCSS VFGMQNNLAPSSSVLSHGTTTASTAYGAKKNVPQPPTVTSTGVSTSATCTTSVQSDDLLHKDCKFLILEK DNTPAKKEMELLIMTKDSGKVFTASPATISSTSFSEDTLKKEKQAAYAADTCLKADVNGDLNTVSTKSKM TSAENHGYDRGGGGGRGKGGGAGGGGGGGGASGGGGAWGAAPAWCPCGSCCSWWKWLLGLLLTWLLLLGL LFGLIALAEEVRKLKARVEELEKTKVLYHDVQMDKSNRDRLQAEAPSLGPGLGKAELDGYSQEAIWLFVR NKLMTEQENGNLRGSPGPKGDMGSQGPKGDRGLPGTPGIPGPLGHPGPEGPKGQKGSIGDPGMEGPIGQR GLAGPMGPRGEPGPPGSGEKGDRGIAGEQGPQGLPGVPGPPGLRGHSGSPGPQGPPGAVGPQGLRGDVGL PGVKGDKGLMGPPGPKGDQGEKGPRGLTGEPGIRGLPGAVGEPGAKGAMGPAGADGQQGSRGEQGLTGMP GTRGPPGPAGDPGKPGLTGPQGPQGLPGSPGRPGTKGEPGAPGRVMTSEGSSTITVPGPPGPPGAMGPPG PPGTPGPAGPAGLPGQQGPRGEPGLAGDSFLSSGSSISEVLSAQGVDLRGPPGPPGPRGPPGPSIPGPPG PRGPPGEGVPGPPGPPGSFLTDSETFFTGPPGPPGPPGPKGDQGDPGVPGTPGISGGLSHGASSSTLYMQ GPPGPPGPPGPPGSLSSSGQDIQHYIAEYMQSDNIRTYLSGVQGPPGPPGPPGPVITITGETFDYSQLAS QVVSYLRSSGYGAGLSSASSSEDILAMLRRNDVWQYLRQNLVGPPGPPGPPGVSGDGSLLSLDYGELSRH ILNYMSSSGISFGHPGPPGPPGLPGTSYEELLTMLRAAGLSSIPGPPGPPGPPGPRGPPGVSAALSTYAA ENSDNFRSELISYLTSPDVRSFIVGPPGPPGPQGPPGDGHLRENYNWSSNSSARRGTSYSSSTGTGGTNG GSLGEGGAYGAGDGGPYGTDIGPGGGYGAAAGGGIYGTNGDSFRDGFTGDLDYNKLAVRVSESMQRQGLL QGMAYTVQGPPGPQGPPGISRVFSAYSNVTQDLMDFFQTYGTIPGPPGQKGDVGTPGPKGDRGPAGPRGP PGPPGPRGNKGEKGDKGDQVYTGRRKRSIAIKP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007732 |
| ORF Size | 4299 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007732.2, NP_031758.2 |
| RefSeq Size | 5428 bp |
| RefSeq ORF | 4302 bp |
| Locus ID | 12821 |
| UniProt ID | Q07563 |
| MW | 144.6 kDa |
| Gene Summary | May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224447 | Col17a1 (untagged) - Mouse collagen, type XVII, alpha 1 (Col17a1), (10ug) |
CNY 18,430.00 |
|
| MG226746 | Col17a1 (tGFP-tagged) - Mouse collagen type XVII alpha 1 (Col17a1), (10ug) |
CNY 11,408.00 |
|
| MR226746L3 | Lenti ORF clone of Col17a1 (Myc-DDK-tagged) - Mouse collagen, type XVII, alpha 1 (Col17a1) |
CNY 12,730.00 |
|
| MR226746L4 | Lenti ORF clone of Col17a1 (mGFP-tagged) - Mouse collagen, type XVII, alpha 1 (Col17a1) |
CNY 12,730.00 |
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