Cspg4 (NM_139001) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4732461B14Rik; AN2; NG2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR226732 representing NM_139001 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCTCGGCCCGGGACACCCGCTGTCAGCTCCAGCCCTGGCCTTGGCTCTTACCTTGGCCTTGTTGG TCAGATCTACAGCTCCTGCCTCCTTCTTCGGGGAGAACCACCTGGAGGTGCCCGTGCCCTCAGCTCTGAC CAGAGTAGACCTGCTGCTCCAGTTCTCCACATCCCAGCCCGAAGCCCTGCTCCTCTTGGCAGCAGGCCAA GATGACCATCTCTTGCTCCAGCTCCACTCAGGATGCCTCCAGGTCAGACTTGCCCTGGGACAGAAGGAGC TAAAGCTGCAGACGCCAGCAGACACCGTGCTGAGTGACTCTGCCCCCCACACTGTAGTGCTCACTGTCTC CGACAGCTGGGCTGTGCTGTCTGTTGACGGAGTGTTGAACACCTCTGCTCCCATCCCAAGAGCATCCCAC CTCAAGGCCACCTACGGGCTCTTCGTGGGCTCCTCTGGAAGCCTTGACCTGCCTTACCTGAAGGGAATCA GCCGACCCCTAAGGGGCTGCCTCCACTCAGCCATTCTCAATGGCCGCAACCTCCTCCGTCCGCTGACCTC CGATGTTCATGAAGGTTGTGCTGAAGAATTCTCTGCTGGTGATGAAGTGGGCCTGGGCTTCTCTGGGCCC CACTCACTGGCTGCCTTCCCTGCCTGGAGCACTCGGGAGGAAGGCACCCTGGAGTTTACGCTCACCACTC GGAGTCAGCAAGCACCCCTGGCCTTCCAAGCAGGGGACAAGCGTGGCAACTTTATCTACGTGGACATATT TGAGGGCCACTTGCGGGCTGTGGTTGAGAAAGGCCAAGGTACCATGCTACTCCGCAACAGCGTGCCTGTG GCTGATGGGCAGCCCCACGAGGTCAGCGTACACATAGATGTTCACCGGCTGGAAATCTCTGTAGACCAAT ACCCTACGCGTACTTTCAACCGTGGGGTCCTCAGCTACCTGGAGCCACGTGGCAGTCTCCTCCTTGGGGG TCTGGACACAGAAGCCTCTCGCCACCTCCAAGAGCACCGTCTGGGCCTGGCACCGGGGGCTGCCAATATC TCCCTGGTAGGCTGCATAGAAGATTTCAGTGTTAATGGCAGGAGGCAGGGCCTCCGGGATGCCTGGCTGA CCCGTGACATGTCAGCAGGATGCAGGCCTGAGGAGGATGAATATGAAGAAGAGGTCTATGGCCCATATGA AACTTTCTCCACCCTGGCACCAGAAGCTTGGCCAGCCATGGAACTGCCAGAGCCATGTATTCCTGAGCCG GGACTGCCTGCTGTCTTTGCAAACTTCACCCAGCTGCTTACCATCAGCCCCCTGGTGGTGGCCGAGGGTG GCACGGCCTGGCTTGAGTGGCGGCACGTGCAGCCCACCCTGGACCTGACAGAGGCAGAGCTGCGCAAGTC CCAGGTGCTGTTCAGCGTGAGCCAGAGTGCACGACATGGTGACCTGGAGCTAGACATCCTGGGAGCCCAA ACCCGGAAAATGTTTACCCTGTTGGACGTGGTGAACCGTAAGGCCCGCTTTGTTCACGATGGCTCTGAAG ACACCTCTGACCAGCTGATGCTGGAGGTGTCTGTGACTGCTCGGGCACCTGTGCCCTCCTGCCTGCGGAG GGGCCAAATTTACATCCTGCCCATCCAGGTCAATCCTGTCAACGATCCACCTCGCATCATCTTCCCCCAC GGCAGCCTCATGGTGATCCTGGAACACACACAGAAGCCTCTGGGACCGGAGATTTTCCAGGCCTACGACC CGGACTCTGCCTGTGAGGGTCTCACCTTCCAGCTCCTTGGCGTCTCCTCTGGTGTCCCTGTGGAACACCG AGACCAGCCAGGAGAGCCAGCAACTGAGTTTTCCTGTCGGGAACTGGAGGTGGGCGACATAGTCTATGTC CACCGTGGTGGGCCTGCACAGGACCTAACATTCCGGGTCAGTGATGGGATGCAGGCCAGTGCTCCAGCGA CACTGAAGGTAGTGGCCGTCCGGCCAGCCATACAGATCCTCCACAACACAGGGCTGCACCTGGCCCAGGG CTCTGCCGCAGCCATCTTGCCTGCCAACCTGTCAGTAGAAACGAACGCGGTGGGACAGGATGTGAGCGTG CTGTTCCGAGTCACTGGTACCTTGCAATTCGGGGAGCTGCAGAAGCAGGGGGCCGGAGGGGTCGAGGGCA CCGAGTGGTGGGACACACTGGCCTTCCACCAGCGCGATGTGGAGCAAGGCCGAGTGAGGTACCTGAGCAC TGACCCACAGCACCACACCCAAGACACAGTGGAGGACCTGATCTTAGAGGTGCAGGTGGGCCAGGAGACA CTGAGCAATCTGTCTTTCCCAGTGACCATCCAGAGGGCCACAGTATGGATGCTGAGGCTTGAACCTCTGC ATACACAGAACCCTCATCAGGAGACCCTCACCCCAGCCCACCTAGAGGCATCCCTGGAGGAAGAGGAGGA AGAGGGAAGCCCACAACCTCATACCTTCCACTATGAGTTGGTTCAGGCCCCCAGAAGAGGCAACCTCCTG CTTCAGGGTACAAGGCTGTCAGACGGCGAGAGCTTCAGCCAGAGTGACCTGCAGGCCGGTCGGGTGACCT ACAGGGCCACAATGCGGACCTCTGAGGCAGCCGATGACTCCTTCCGTTTCCGGGTCACATCCCCACCCCA TTTCTCCCCGCTCTACACATTCCCCATCCACATTGGTGGTGACCCAAATGCTCCCGTCCTCACTAACGTC CTTCTCATGGTCCCCGAGGGAGGGGAGGGCGTGCTGTCTGCCGACCACCTCTTCGTCAAGAGTCTCAACA GCGCCAGCTATCTCTATGAGGTGATGGAGCAGCCTCACCATGGCAAGTTGGCTTGGAGGGACCCAAAGGG GAAGTCCACCCCAGTGACATCCTTCACTAATGAGGACCTACTTCATGGCCGACTGGTCTACCAGCACGAT GACTCTGAGACCATAGAGGATGATATCCCGTTTGTGGCCACACGCCAGGGAGAGGGCAGTGGTGACATGG CCTGGGAGGAGGTGCGTGGTGTCTTCCGAGTGGCCATCCAGCCTGTGAATGACCACGCCCCTGTGCAGAC CATTAGCCGTGTCTTCCACGTGGCCCGGGGTGGACAGCGACTGCTGACTACAGATGATGTAGCCTTCAGC GATGCTGATTCAGGGTTCAGTGATGCGCAACTGGTGCTGACCCGCAAGGACCTCCTCTTTGGCAGCATTG TGGCTATGGAGGAGCCCACGAGGCCCATCTACCGGTTCACCCAAGAGGATCTCAGGAAGAAGCAAGTCCT GTTTGTGCACTCGGGGGCCGACCACGGCTGGCTCCAGCTGCAGGTGTCTGATGGGCAGCACCAGGCTACT GCCATGCTGGAGGTCCAAGCCTCAGAACCCTATCTTCACGTAGCCAATAGTTCTAGTCTCGTGGTTCCTC AAGGAGGCCAGGGCACCATTGACACTGCTGTGCTCCAGCTGGACACCAACCTGGACATACGAAGTGGAAA TGAGGTCCACTACCATGTAACCGCTGGTCCTCAGTGGGGGCAGCTGCTCCGGGATGGCCAGTCAGTCACC TCCTTCTCCCAACGGGACTTGCTCGATGGGGCCATCCTCTACAGCCACAATGGCAGCCTCAGCCCCCAAG ACACCCTGGCGTTTTCTGTGGCAGCAGGGCCAGTACACACTAACACCTTCCTACAAGTGACCATTGCCCT CGAGGGTCCCCTGGCCCCACTGCAACTGGTGCAGCACAAAAAGATCTACGTCTTCCAGGGGGAAGCGGCT GAGATCAGAAGGGACCAGCTAGAGGTGGTCCAGGAGGCAGTGCTGCCTGCCGACATCATGTTCTCGTTGA GAAGCCCCCCGAACGCCGGCTACCTGGTGATGGTGTCCCACGGCGCTTCAGCAGAGGAGCCGCCCAGCCT GGACCCTGTGCAGAGCTTCTCCCAGGAGGCAGTGAATTCAGGCCGGGTTCTCTACCTGCACTCTCGCCCT GGAGCCTGGAGTGATTCCTTCTCCCTGGATGTGGCCTCGGGCCTGGGTGATCCTCTTGAAGGCATCTCTG TGGAGCTGGAGGTGCTGCCCACAGTCATCCCCCTGGATGTTCAAAACTTCAGCGTTCCCGAGGGCGGCAC CCGGACGCTGGCCCCTCCTCTGGTCCAGATCACTGGGCCTTACTTCCCCACGCTGCCAGGCCTTGTCCTG CAGGTGCTAGAGCCACCGCAGCACGGGGCCCTGCAGAAGGAGGATCATTCTCAAGATGGGAGCCTCAGCA CCTTCTCCTGGAGAGAGGTGGAAGAGCAGCTGATCCGCTACGTGCATGATGGAAGTGAGACACAGACAGA CGCCTTTGTTCTGCTAGCTAATGCCTCAGAGATGGACCGCCAGAGCCAGCCCGTGGCCTTCACGATCACC ATCCTTCCTGTTAATGACCAACCCCCTGTTCTCACCACAAACACAGGCCTGCAAATCTGGGAGGGGGCCA TTGTGCCCATCCCTCCTGAGGCCCTCAGGGGAACAGACAATGATTCGGGCCCAGAAGACTTGGTCTACAC CATCGAGCAGCCCAGCAACGGACGGATCGCCTTGAGGGTGGCACCAGACACCGAGGTCCACCGCTTCACA CAGGCCCAGCTGGACAGTGGGCTTGTGCTGTTCTCACACAGAGGAGCCCTGGAAGGAGGCTTCCACTTCG ACCTCTCTGACGGCGCACACACTTCTCCTGGACATTTCTTCCGAGTGGTGGCCCAGAAGCAGGCACTCCT CTCCTTGGAGGGCACCCGGAAGTTGACCGTCTGTCCAGAGTCTGTGCAGCCGCTCAGCAGCCAGAGCCTG AGCGCCAGTTCCAGCACAGGCGCTGACCCTCGTCACCTGCTCTACCGGGTGGTAAGAGGCCCCCAGCTTG GCCGACTCCTCCATGCCCAGCAAGGAAGTGCAGAGGAGGTCTTGGTGAACTTCACCCAGGCTGAGGTAAA TGCTGGGAATATTCTGTATGAGCACGAGATGTCCTCTGAGCCCTTCTGGGAAGCCCATGACACCATCGGT CTCCTACTGTCCTCACCACCTGCCAGGGATCTGGCTGCCACCCTGGCCGTGATGGTGTCTTTCGATGCTG CCTGTCCCCAGCGCCCCAGCCGTCTCTGGAAGAACAAAGGTCTTTGGGTCCCCGAGGGCCAGCGGGCCAA GATCACTGTGGCTGCCCTTGATGCCGCCAACCTCCTAGCCAGTGTGCCAGCATCTCAGCGCAGTCGGCAT GATGTACTCTTCCAGGTCACACAGTTCCCCACCCGAGGCCAGCTCCTGGTGTCTGAGGAGCCACTCCATG CCAGGAGGCCCTACTTCCTGCAATCTGAGCTGGCCGCGGGACAGCTGGTGTACGCCCATGGTGGTGGGGG CACGCAGCAGGATGGCTTCCGCTTCCGTGCCCACCTCCAGGGACCGACGGGGACTTCTGTGGCAGGACCC CAGACCTCTGAGGCCTTTGTCATCACTGTGAGGGACGTGAATGAGCGGCCCCCTCAGCCACAGGCCTCCA TTCCTCTCCGTGTCACCAGGGGCTCACGAGCCCCTGTATCTCGAGCCCAGCTGAGTGTAGTCGACCCAGA CTCAGCTCCAGGGGAAATTGAATATGAAGTACAGAGGGCACCCCACAATGGCTTCCTGAGCCTGGCTGGG GACAACACTGGACCTGTGACTCACTTCACACAGGCTGATGTGGATGCAGGGCGACTGGCCTTCGTGGCAA ATGGGAGCAGTGTGGCCGGCGTCTTCCAGTTGAGCATGTCTGATGGAGCCAGCCCCCCCATACCCATGTC CCTGGCTGTGGATGTCTTGCCATCCACCATTGAGGTGCAGCTGCGTGCACCCCTGGAGGTGCCCCAAGCT CTAGGACGTACCTCACTGAGCCGGCAGCAGCTTCAGGTTATTTCCGATCGTGAGGAACCAGATGTAGCTT ACCGCCTCACTCAGGGGCCCCTGTATGGGCAGCTACTAGTAGGGGGGCAGCCTGCCTCCGCCTTCAGCCA GCTGCAGGTAGACCAGGGGGACGTGGTCTTTGTCTTCACCAACTTCTCTTCCTCTCAGGATCACTTCAAA GTTGTAGCTCTGGCCAGGGGTGTGAACGCATCAGCCACCGTAAATGTCACAGTGCAGGCTCTGTTGCATG TGTGGGCTGGTGGGCCATGGCCTCAGGGTACCACCCTGCGCCTTGACCCCACTGTGCTCGATGCCAGTGA GCTGGCCAACCGCACAGGCAGTATGCCCCACTTCCGGCTCCTGGCAGGACCCCGCTATGGCCGTGTGGTC CGAGTGTCCCAAGGCCGAACAGAATCTAGGAGCAACCAACTTGTGGAACATTTCACTCAGCGGGACCTGG AAGAGGGACAGCTGGGGCTAGAGGTGGGCAAGCCAGAGGGCAGGTCCACTGGCCCAGCAGGTGACAGACT TACTCTGGAGCTGTGGGCAAAGGGTGTCCCACCTGCTGTGGCCTTGCTGGACTTTGCCACTGAGCCTTAC CATGCGGCCAAATCCTACAGTGTGGCCCTACTCAGTGTCCCTGAGGCTGTTCGTACAGAAACAGAGAAAC CAGGAAGAAGCGTCCCCACTGGCCAGCCAGGCCAGGCAGCATCCAGCCCCGTGCCCACTGCGGCCAAAGG TGGTTTCCTGGGCTTCTTAGAGGCCAACATGTTCAGCATCATCATTCCGGTGTGCCTGATCCTCCTGCTC CTGGCCCTTATCTTGCCTCTGCTCTTCTACCTCCGCAAACGCAACAAGACGGGCAAGCACGATGTCCAGG TGTTGACCGCCAAACCCCGCAATGGCCTAGCCGGTGACACAGAGACCTTTCGAAAGGTAGAGCCAGGCCA AGCTATTCCACTCATAACTGTGCCTGGCCAGGGGCCCCCACCAGGAGGCCAGCCTGACCCAGAACTATTG CAGTTTTGCCGGACACCCAATCCTGCCCTCAGGAATGGCCAGTACTGGGTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR226732 representing NM_139001 Red=Cloning site Green=Tags(s) MLLGPGHPLSAPALALALTLALLVRSTAPASFFGENHLEVPVPSALTRVDLLLQFSTSQPEALLLLAAGQ DDHLLLQLHSGCLQVRLALGQKELKLQTPADTVLSDSAPHTVVLTVSDSWAVLSVDGVLNTSAPIPRASH LKATYGLFVGSSGSLDLPYLKGISRPLRGCLHSAILNGRNLLRPLTSDVHEGCAEEFSAGDEVGLGFSGP HSLAAFPAWSTREEGTLEFTLTTRSQQAPLAFQAGDKRGNFIYVDIFEGHLRAVVEKGQGTMLLRNSVPV ADGQPHEVSVHIDVHRLEISVDQYPTRTFNRGVLSYLEPRGSLLLGGLDTEASRHLQEHRLGLAPGAANI SLVGCIEDFSVNGRRQGLRDAWLTRDMSAGCRPEEDEYEEEVYGPYETFSTLAPEAWPAMELPEPCIPEP GLPAVFANFTQLLTISPLVVAEGGTAWLEWRHVQPTLDLTEAELRKSQVLFSVSQSARHGDLELDILGAQ TRKMFTLLDVVNRKARFVHDGSEDTSDQLMLEVSVTARAPVPSCLRRGQIYILPIQVNPVNDPPRIIFPH GSLMVILEHTQKPLGPEIFQAYDPDSACEGLTFQLLGVSSGVPVEHRDQPGEPATEFSCRELEVGDIVYV HRGGPAQDLTFRVSDGMQASAPATLKVVAVRPAIQILHNTGLHLAQGSAAAILPANLSVETNAVGQDVSV LFRVTGTLQFGELQKQGAGGVEGTEWWDTLAFHQRDVEQGRVRYLSTDPQHHTQDTVEDLILEVQVGQET LSNLSFPVTIQRATVWMLRLEPLHTQNPHQETLTPAHLEASLEEEEEEGSPQPHTFHYELVQAPRRGNLL LQGTRLSDGESFSQSDLQAGRVTYRATMRTSEAADDSFRFRVTSPPHFSPLYTFPIHIGGDPNAPVLTNV LLMVPEGGEGVLSADHLFVKSLNSASYLYEVMEQPHHGKLAWRDPKGKSTPVTSFTNEDLLHGRLVYQHD DSETIEDDIPFVATRQGEGSGDMAWEEVRGVFRVAIQPVNDHAPVQTISRVFHVARGGQRLLTTDDVAFS DADSGFSDAQLVLTRKDLLFGSIVAMEEPTRPIYRFTQEDLRKKQVLFVHSGADHGWLQLQVSDGQHQAT AMLEVQASEPYLHVANSSSLVVPQGGQGTIDTAVLQLDTNLDIRSGNEVHYHVTAGPQWGQLLRDGQSVT SFSQRDLLDGAILYSHNGSLSPQDTLAFSVAAGPVHTNTFLQVTIALEGPLAPLQLVQHKKIYVFQGEAA EIRRDQLEVVQEAVLPADIMFSLRSPPNAGYLVMVSHGASAEEPPSLDPVQSFSQEAVNSGRVLYLHSRP GAWSDSFSLDVASGLGDPLEGISVELEVLPTVIPLDVQNFSVPEGGTRTLAPPLVQITGPYFPTLPGLVL QVLEPPQHGALQKEDHSQDGSLSTFSWREVEEQLIRYVHDGSETQTDAFVLLANASEMDRQSQPVAFTIT ILPVNDQPPVLTTNTGLQIWEGAIVPIPPEALRGTDNDSGPEDLVYTIEQPSNGRIALRVAPDTEVHRFT QAQLDSGLVLFSHRGALEGGFHFDLSDGAHTSPGHFFRVVAQKQALLSLEGTRKLTVCPESVQPLSSQSL SASSSTGADPRHLLYRVVRGPQLGRLLHAQQGSAEEVLVNFTQAEVNAGNILYEHEMSSEPFWEAHDTIG LLLSSPPARDLAATLAVMVSFDAACPQRPSRLWKNKGLWVPEGQRAKITVAALDAANLLASVPASQRSRH DVLFQVTQFPTRGQLLVSEEPLHARRPYFLQSELAAGQLVYAHGGGGTQQDGFRFRAHLQGPTGTSVAGP QTSEAFVITVRDVNERPPQPQASIPLRVTRGSRAPVSRAQLSVVDPDSAPGEIEYEVQRAPHNGFLSLAG DNTGPVTHFTQADVDAGRLAFVANGSSVAGVFQLSMSDGASPPIPMSLAVDVLPSTIEVQLRAPLEVPQA LGRTSLSRQQLQVISDREEPDVAYRLTQGPLYGQLLVGGQPASAFSQLQVDQGDVVFVFTNFSSSQDHFK VVALARGVNASATVNVTVQALLHVWAGGPWPQGTTLRLDPTVLDASELANRTGSMPHFRLLAGPRYGRVV RVSQGRTESRSNQLVEHFTQRDLEEGQLGLEVGKPEGRSTGPAGDRLTLELWAKGVPPAVALLDFATEPY HAAKSYSVALLSVPEAVRTETEKPGRSVPTGQPGQAASSPVPTAAKGGFLGFLEANMFSIIIPVCLILLL LALILPLLFYLRKRNKTGKHDVQVLTAKPRNGLAGDTETFRKVEPGQAIPLITVPGQGPPPGGQPDPELL QFCRTPNPALRNGQYWV SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_139001
ORF Size	6981 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_139001.2, NP_620570.2
RefSeq Size	8050 bp
RefSeq ORF	6984 bp
Locus ID	121021
UniProt ID	Q8VHY0
MW	252.8 kDa
Gene Summary	Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.[UniProtKB/Swiss-Prot Function]

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