Ret (NM_009050) Mouse Tagged ORF Clone
CAT#: MR226724
- TrueORF®
Ret (Myc-DDK-tagged) - Mouse ret proto-oncogene (Ret), transcript variant 2
ORF Plasmid: tGFP
"NM_009050" in other vectors (3)
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CNY 11,648.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | c-Ret; PTC; RET9; RET51 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226724 representing NM_009050
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAAAGCGACGTCCGGCGCCGCAGGGCTGGGGCTGAAGCTGATTTTGCTCCTGCCGCTGCTAGGAG AAGCCCCACTGGGCCTCTATTTCTCAAGGGATGCTTACTGGGAGAGGCTGTATGTAGACCAGCCAGCTGG CACACCTCTGCTCTATGTCCATGCCCTACGGGATGCCCCTGGAGAAGTGCCGAGCTTCCGCCTGGGCCAG CATCTCTATGGCGTCTACCGTACACGGCTGCATGAGAATGACTGGATCCGCATCAATGAGACTACTGGCC TTCTCTACCTCAATCAGAGCCTGGACCACAGTTCCTGGGAACAGCTCAGCATCCGCAATGGTGGTTTCCC CCTGCTCACCATCTTCCTCCAGGTCTTTCTGGGGTCCACAGCCCAGAGAGAGGGAGAATGCCATTGGCCA GGCTGTACCCGTGTGTACTTCTCCTTCATCAACGACACCTTCCCAAATTGTAGCTCCTTCAAAGCCCAGG ATCTCTGCATCCCAGAGACAGCCGTGTCCTTCCGAGTCAGGGAGAACAGGCCTCCTGGCACCTTCTACCA CTTCCACATGTTACCCGTGCAGTTCCTTTGTCCCAACATCAGTGTGAAGTACAGTCTCTTAGGAGGGGAT AGTCTGCCCTTCCGTTGTGACCCAGACTGCCTGGAGGTGAGCACTCGCTGGGCCCTGGATCGAGAGCTCC GGGAGAAGTATGTGCTGGAGGCTTTGTGCATAGTGGCAGGCCCTGGTGCCAACAAAGAGACGGTGACTCT GTCCTTCCCAGTGACAGTGTATGATGAGGACGACTCGGCGCCCACCTTCTCTGGAGGTGTGGGCACTGCC AGCGCGGTGGTGGAGTTTAAGCGGAAGGAGGGCACTGTGGTGGCCACCCTGCAGGTGTTCGATGCAGATG TGGTGCCAGCGTCTGGGGAGCTGGTGAGACGGTACACAAACACACTCCTCTCAGGGGACTCCTGGGCCCA GCAGACCTTCCGGGTGGAGCATTCGCCCATCGAGACCTTGGTCCAGGTCAACAACAACTCCGTTCGGGCA ACCATGCACAATTACAAGCTGATTCTCAACAGGAGCCTGTCTATCTCAGAGAGCCGAGTCCTGCAGCTCG CGGTCCTGGTCAACGACTCAGACTTCCAGGGGCCTGGGGCAGGTGGTATCCTCGTCCTCCATTTCAACGT GTCTGTACTGCCCGTCACCCTGAACCTACCCAGGGCCTACTCCTTCCCAGTGAATAAGAGGGCCCGCCGC TATGCCCAGATCGGGAAAGTCTGTGTGGAAAACTGCCAGGAGTTCAGCGGTGTCTCCATCCAGTACAAGC TGCAGCCTTCCAGCATCAACTGCACTGCCCTAGGTGTGGTCACCTCACCCGAGGACACCTCGGGGACCCT ATTTGTAAATGACACAGAGGCCCTGCGGCGACCTGAGTGCACCAAGCTTCAGTACACGGTGGTAGCCACT GACCGGCAGACCCGCAGACAGACCCAGGCTTCGCTAGTGGTCACTGTGGAGGGGACATCCATTACTGAAG AAGTAGGCTGCCCCAAGTCCTGTGCAGTAAACAAGAGGCGCCCCGAGTGTGAGGAATGTGGTGGCCTGGG TTCTCCAACTGGCAGGTGCGAGTGGCGCCAGGGAGATGGTAAAGGGATCACCAGGAACTTCTCCACCTGC TCCCCCAGTACCAGGACCTGCCCCGACGGCCACTGTGATGCTGTGGAGAGCCGGGATGCCAACATTTGCC CCCAGGACTGTCTCCGTGCCGACATTGTTGGAGGACACGAGCGAGGGGAGCGCCAGGGCATTAAAGCAGG CTACGGCATCTGCAACTGTTTCCCTGATGAGAAGAAATGCTTCTGCGAGCCAGAGGACAGCCAGGGCCCA CTGTGTGATGCGCTGTGCCGCACGATCATCACAGCTGCCCTCTTCTCCCTTATCATCTCCATCCTGCTGT CCATCTTCTGTGTCTGCCACCACCACAAGCATGGGCACAAGCCGCCCATTGCATCAGCGGAAATGACCTT CTGCCGGCCGGCCCAGGGCTTCCCAATCAGTTATTCCTCCTCAGGCACCCGCCGGCCCTCACTGGATTCC ACGGAGAACCAGGTTCCTGTGGACTCTTTCAAGATCCCGGAGGATCCAAAGTGGGAATTTCCTCGGAAGA ACTTAGTTCTTGGGAAAACTCTGGGAGAAGGCGAGTTTGGAAAAGTTGTCAAGGCCACAGCCTTCCGTCT GAAAGGCCGGGCAGGATACACCACAGTGGCTGTGAAAATGCTGAAAGAAAACGCCTCCCAGAGTGAGTTA CGAGACCTGCTGTCTGAGTTCAACCTTCTGAAACAAGTCAACCATCCACATGTCATCAAGTTGTATGGGG CCTGCAGCCAGGATGGGCCACTTCTTCTCATTGTGGAGTATGCCAAGTATGGCTCCCTGCGGGGATTCCT CCGTGACAGCCGCAAGATTGGGCCTGCCTATGTGAGCGGTGGAGGCAGCCGCAACTCCAGCTCGCTGGAC CACCCAGATGAAAGGGTACTGACCATGGGTGACCTCATCTCCTTCGCCTGGCAGATCTCGAGGGGTATGC AGTACTTGGCAGAAATGAAGCTTGTACATCGGGACTTAGCTGCCAGGAACATCTTGGTGGCTGAGGGACG GAAGATGAAGATTTCCGACTTTGGGCTGTCCCGAGATGTTTATGAGGAAGATTCCTATGTGAAGAAAAGC AAGGGCCGGATTCCCGTCAAGTGGATGGCAATTGAGTCCCTTTTCGATCACATCTATACTACTCAAAGTG ATGTGTGGTCCTTTGGAGTGCTGCTCTGGGAGATTGTGACCCTGGGAGGCAACCCCTACCCTGGAATTCC TCCTGAACGACTCTTCAACCTTCTGAAGACAGGCCACAGGATGGAGAGGCCAGACAACTGCAGCGAGGAA ATGTACCGTCTGATGCTGCAGTGCTGGAAGCAGGAGCCAGACAAGAGGCCAGTGTTTGCTGACATCAGCA AGGATCTGGAGAAGATGATGGTCAAGAGCAGAGACTACTTGGACCTGGCTGCATCCACACCTTCGGACTC ACTGCTGTATGACGATGGGCTCTCAGAAGAGGAGACACCCCTGGTGGACTGTAACAATGCTCCCCTCCCG CGCTCCCTCCCTTCCACATGGATTGAAAACAAACTCTATGGCATGTCAGACCCGAACTGGCCTGGAGAGA GTCCTGTACCACTCACGAGAGCCGATGGCACTAGCACTGGGTTCCCAAGATATGCAAATGATAGTGTATA TGCTAACTGGATGGTTTCACCCTCAGCGGCAAAATTAATGGACACATTTGATAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226724 representing NM_009050
Red=Cloning site Green=Tags(s) MAKATSGAAGLGLKLILLLPLLGEAPLGLYFSRDAYWERLYVDQPAGTPLLYVHALRDAPGEVPSFRLGQ HLYGVYRTRLHENDWIRINETTGLLYLNQSLDHSSWEQLSIRNGGFPLLTIFLQVFLGSTAQREGECHWP GCTRVYFSFINDTFPNCSSFKAQDLCIPETAVSFRVRENRPPGTFYHFHMLPVQFLCPNISVKYSLLGGD SLPFRCDPDCLEVSTRWALDRELREKYVLEALCIVAGPGANKETVTLSFPVTVYDEDDSAPTFSGGVGTA SAVVEFKRKEGTVVATLQVFDADVVPASGELVRRYTNTLLSGDSWAQQTFRVEHSPIETLVQVNNNSVRA TMHNYKLILNRSLSISESRVLQLAVLVNDSDFQGPGAGGILVLHFNVSVLPVTLNLPRAYSFPVNKRARR YAQIGKVCVENCQEFSGVSIQYKLQPSSINCTALGVVTSPEDTSGTLFVNDTEALRRPECTKLQYTVVAT DRQTRRQTQASLVVTVEGTSITEEVGCPKSCAVNKRRPECEECGGLGSPTGRCEWRQGDGKGITRNFSTC SPSTRTCPDGHCDAVESRDANICPQDCLRADIVGGHERGERQGIKAGYGICNCFPDEKKCFCEPEDSQGP LCDALCRTIITAALFSLIISILLSIFCVCHHHKHGHKPPIASAEMTFCRPAQGFPISYSSSGTRRPSLDS TENQVPVDSFKIPEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASQSEL RDLLSEFNLLKQVNHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLRDSRKIGPAYVSGGGSRNSSSLD HPDERVLTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRDVYEEDSYVKKS KGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEE MYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYLDLAASTPSDSLLYDDGLSEEETPLVDCNNAPLP RSLPSTWIENKLYGMSDPNWPGESPVPLTRADGTSTGFPRYANDSVYANWMVSPSAAKLMDTFDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009050 |
ORF Size | 3345 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009050.2, NP_033076.2 |
RefSeq Size | 6064 bp |
RefSeq ORF | 3348 bp |
Locus ID | 19713 |
UniProt ID | P35546 |
MW | 124.3 kDa |
Gene Summary | Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis; involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor; in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers; these diseases are characterized by aberrant integrins-regulated cell migration. Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell-signaling in the brainstem which induces inhibition of food-intake. Activates MAPK- and AKT-signaling pathways (PubMed:28846099).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Planar cell polarity genes in motor axon guidance in the limb
,Chai, G;,
Thesis
[RET]
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Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG226724 | Ret (tGFP-tagged) - Mouse ret proto-oncogene (Ret) transcript variant 2, (10ug) |
CNY 12,260.00 |
|
MR226724L3 | Lenti ORF clone of Ret (Myc-DDK-tagged) - Mouse ret proto-oncogene (Ret), transcript variant 2 |
CNY 13,020.00 |
|
MR226724L4 | Lenti ORF clone of Ret (mGFP-tagged) - Mouse ret proto-oncogene (Ret), transcript variant 2 |
CNY 13,020.00 |