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F9 (NM_007979) Mouse Tagged ORF Clone
CAT＃: MR226536
- TrueORF®
F9 (Myc-DDK-tagged) - Mouse coagulation factor IX (F9)
ORF Plasmid: tGFP
"NM_007979" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW111646; Cf-9; Cf9 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226536 representing NM_007979
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCACCTGAACACCGTCATGGCAGAATCCCCGGCTCTCATCACCATCTTCCTTTTAGGATATCTAC TCAGTACCGAATGTGCAGTTTTCCTTGATCGTGAAAATGCCACCAAAATTCTTACCCGTCCAAAGAGATA TAATTCAGGAAAACTAGAAGAGTTTGTTCGAGGAAACCTTGAAAGAGAGTGTATAGAAGAAAGATGTAGT TTTGAAGAAGCACGAGAAGTTTTTGAAAACACTGAAAAAACTACTGAATTTTGGAAGCAGTATGTTGATG GAGATCAGTGTGAATCAAATCCTTGTTTAAATGGTGGAATATGCAAGGATGATATTAGTTCCTATGAATG CTGGTGCCAAGTTGGATTTGAAGGAAGGAACTGTGAATTAGATGCAACGTGTAACATTAAAAATGGCAGG TGCAAGCAGTTTTGTAAAAACAGTCCTGATAACAAGGTAATTTGTTCCTGCACTGAGGGATACCAACTTG CAGAAGACCAGAAGTCCTGTGAACCAACAGTTCCATTTCCATGTGGGAGAGCTTCTATTTCATACAGTTC TAAAAAGATCACGAGAGCTGAGACTGTTTTCTCTAATATGGACTATGAAAATTCTACTGAAGCTGTATTC ATTCAAGATGACATCACTGATGGTGCCATTCTTAATAACGTCACTGAAAGTAGTGAATCACTTAATGACT TCACTCGAGTTGTTGGTGGAGAAAACGCAAAACCGGGTCAAATCCCTTGGCAGGTCATTTTAAATGGTGA AATTGAGGCATTCTGTGGAGGTGCCATCATTAATGAAAAATGGATTGTAACTGCTGCCCACTGTCTTAAA CCTGGTGATAAAATTGAGGTTGTTGCTGGTGAATATAACATTGATAAGAAGGAAGACACAGAACAAAGGA GAAATGTGATTCGAACTATCCCTCATCACCAGTACAATGCAACTATTAATAAGTATAGTCATGACATTGC CTTGCTGGAACTGGATAAACCTTTAATACTAAACAGCTATGTAACACCTATCTGTGTTGCCAATAGGGAA TATACAAATATCTTCCTCAAGTTTGGTTCTGGCTATGTCAGTGGCTGGGGAAAAGTCTTCAACAAAGGGA GACAGGCTTCCATTCTTCAGTACCTTAGAGTTCCACTGGTGGATAGAGCCACATGCCTTAGGTCCACAAC ATTCACTATCTATAACAACATGTTCTGTGCAGGCTACCGTGAAGGAGGCAAAGATTCGTGTGAAGGAGAT AGTGGGGGACCCCATGTTACTGAAGTAGAAGGGACAAGTTTCTTAACTGGCATTATTAGCTGGGGTGAAG AATGTGCAATGAAAGGCAAATATGGAATATATACTAAGGTTTCCCGGTACGTCAACTGGATTAAGGAAAA AACAAAGCTAACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226536 representing NM_007979
Red=Cloning site Green=Tags(s) MKHLNTVMAESPALITIFLLGYLLSTECAVFLDRENATKILTRPKRYNSGKLEEFVRGNLERECIEERCS FEEAREVFENTEKTTEFWKQYVDGDQCESNPCLNGGICKDDISSYECWCQVGFEGRNCELDATCNIKNGR CKQFCKNSPDNKVICSCTEGYQLAEDQKSCEPTVPFPCGRASISYSSKKITRAETVFSNMDYENSTEAVF IQDDITDGAILNNVTESSESLNDFTRVVGGENAKPGQIPWQVILNGEIEAFCGGAIINEKWIVTAAHCLK PGDKIEVVAGEYNIDKKEDTEQRRNVIRTIPHHQYNATINKYSHDIALLELDKPLILNSYVTPICVANRE YTNIFLKFGSGYVSGWGKVFNKGRQASILQYLRVPLVDRATCLRSTTFTIYNNMFCAGYREGGKDSCEGD SGGPHVTEVEGTSFLTGIISWGEECAMKGKYGIYTKVSRYVNWIKEKTKLT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007979 |
| ORF Size | 1413 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007979.2, NP_032005.1 |
| RefSeq Size | 2733 bp |
| RefSeq ORF | 1416 bp |
| Locus ID | 14071 |
| UniProt ID | P16294 |
| MW | 53.4 kDa |
| Gene Summary | This gene encodes a vitamin K-dependent serine protease that plays a critical role in the intrinsic pathway of blood coagulation. The encoded protein is an inactive zymogen that is activated by coagulation factor XIa to generate factor IXa, a heterodimer containing heavy and light chains. In association with factor VIII, membrane phospholipids and calcium ions, factor IXa cleaves the inactive zymogen factor X to generate active factor Xa. Genetic deletion of this gene in mice results in a severe bleeding phenotype. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216451 | F9 (untagged) - Mouse coagulation factor IX (F9), (10ug) |
CNY 3,656.00 |
|
| MG226536 | F9 (tGFP-tagged) - Mouse coagulation factor IX (F9), (10ug) |
CNY 3,710.00 |
|
| MR226536L3 | Lenti ORF clone of F9 (Myc-DDK-tagged) - Mouse coagulation factor IX (F9) |
CNY 5,230.00 |
|
| MR226536L4 | Lenti ORF clone of F9 (mGFP-tagged) - Mouse coagulation factor IX (F9) |
CNY 5,230.00 |
