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Mep1b (NM_008586) Mouse Tagged ORF Clone
CAT＃: MR226519
- TrueORF®
Mep1b (Myc-DDK-tagged) - Mouse meprin 1 beta (Mep1b)
ORF Plasmid: tGFP
"NM_008586" in other vectors (4)
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CNY 7,736.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Mep-1b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226519 representing NM_008586
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCCCGGCATCAGCCTTGGTTTCTGGTTTTTGCCACATTTCTCCTGGTTTCTGGTTTGCCAGCTC CGGAAAAGTTTGTCAAAGACATAGATGGAGGAATTGACCAAGACATATTTGATATTAACCAAGGTTTGGG TCTGGACCTTTTTGAGGGAGACATCAAACTCGAGGCAAATGGGAAGAATTCCATCATTGGAGACCACAAG AGATGGCCACATACCATTCCATATGTTCTAGAGGACAGCTTGGAAATGAATGCTAAAGGAGTTATCCTCA ATGCTTTTGAGCGCTATCGCCTTAAAACATGCATTGACTTCAAGCCTTGGTCCGGAGAAGCTAACTATAT CTCAGTGTTCAAGGGCAGTGGGTGCTGGTCTTCAGTGGGAAACATTCATGCTGGGAAGCAGGAGTTGTCC ATTGGGACAAACTGTGACAGAATAGCAACAGTTCAGCATGAGTTCCTCCACGCCCTGGGATTCTGGCATG AGCAGTCACGTGCTGACCGGGATGATTACGTCATTATAGTTTGGGACAGGATTCAGCCAGGCAAGGAACA CAACTTCAACATCTACAATGACAGTGTGTCTGATTCCCTGAATGTCCCATATGACTACACCTCAGTAATG CACTACAGTAAAACCGCTTTCCAGAACGGGACAGAGTCTACCATCGTCACGAGAATCTCAGAGTTCGAGG ACGTGATTGGCCAACGAATGGACTTCAGTGACTATGACCTTCTGAAGCTAAATCAGCTGTATAACTGCAC TTCTTCCTTGAGTTTTATGGACTCCTGTGATTTTGAATTGGAAAATATCTGTGGCATGATCCAAAGTTCG GGGGATAGTGCTGACTGGCAGCGGGTTTCACAGGTTCTCAGTGGCCCAGAGAGCGACCACTCCAAGATGG GCCAGTGCAAAGACTCTGGCTTCTTCATGCATTTCAACACTAGCATTTTGAATGAGGGGGCCACGGCGAT GTTGGAGAGCAGACTGTTGTACCCCAAGAGAGGGTTTCAGTGCTTGGAGTTTTATCTGTACAACAGTGGA AGTGGAAATGACCAACTGAACATTTACACCCGGGAGTACACTACAGGCCAGCAGGGTGGTGTTTTAACCC TTCAGAGACAAATAAAAGAGGTACCCATTGGGAGCTGGCAACTTCACTATGTAACACTGCAAGTGACTAA AAAGTTTCGAGTGGTGTTTGAAGGACTCAGAGGCCCTGGCACGTCATCAGGTGGTCTGTCTATCGATGAC ATCAATCTCTCAGAAACAAGGTGTCCTCACCATATCTGGCACATACAGAATTTCACACAGATTCTAGGCG GCCAGGACACATCTGTATACAGTCCTCCATTTTATTCTTCTAAAGGTTACGCTTTTCAGATCTACATGGA TCTAAGATCCTCAACAAATGTAGGAATTTATTTCCACCTGATCTCCGGTGCCAATGATGATCAATTACAG TGGCCATGTCCTTGGCAACAAGCTACAATGACACTCTTGGATCAAAATCCTGACATCCGACAGCGTATGT TCAACCAGCGGAGTATAACCACAGACCCAACGATGACCAGTGATAATGGAAGCTACTTTTGGGACAGGCC TTCCAAGGTGGGAGTGACGGATGTTTTCCCTAACGGAACTCAGTTTAGCAGAGGTATAGGCTATGGAACG ACTGTCTTCATAACCCGAGAGAGGCTGAAGAGCAGAGAGTTCATAAAAGGAGATGACATTTACATCTTAC TGACTGTTGAAGACATATCTCACCTCAATTCTACATCAGCTGTCCCCGACCCAGTCCCCACCTTGGCTGT CCATAATGCCTGCTCTGAGGTTGTATGTCAGAACGGTGGCATCTGTGTTGTCCAAGATGGCAGAGCTGAG TGCAAGTGTCCTGCAGGAGAAGACTGGTGGTACATGGGCAAAAGGTGTGAGAAGAGAGGGTCCACCCGAG ACACTGTTATCATCGCTGTTTCTTCCACGGTCACCGTGTTTGCTGTGATGCTAATCATCACTCTCGTCAG TGTCTACTGCACCAGGAGGAAATATCGTAAGAAGGCTAGAGCAAATACGGCAGCCATGACTCTAGAAAAC CAACATGCGTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226519 representing NM_008586
Red=Cloning site Green=Tags(s) MDARHQPWFLVFATFLLVSGLPAPEKFVKDIDGGIDQDIFDINQGLGLDLFEGDIKLEANGKNSIIGDHK RWPHTIPYVLEDSLEMNAKGVILNAFERYRLKTCIDFKPWSGEANYISVFKGSGCWSSVGNIHAGKQELS IGTNCDRIATVQHEFLHALGFWHEQSRADRDDYVIIVWDRIQPGKEHNFNIYNDSVSDSLNVPYDYTSVM HYSKTAFQNGTESTIVTRISEFEDVIGQRMDFSDYDLLKLNQLYNCTSSLSFMDSCDFELENICGMIQSS GDSADWQRVSQVLSGPESDHSKMGQCKDSGFFMHFNTSILNEGATAMLESRLLYPKRGFQCLEFYLYNSG SGNDQLNIYTREYTTGQQGGVLTLQRQIKEVPIGSWQLHYVTLQVTKKFRVVFEGLRGPGTSSGGLSIDD INLSETRCPHHIWHIQNFTQILGGQDTSVYSPPFYSSKGYAFQIYMDLRSSTNVGIYFHLISGANDDQLQ WPCPWQQATMTLLDQNPDIRQRMFNQRSITTDPTMTSDNGSYFWDRPSKVGVTDVFPNGTQFSRGIGYGT TVFITRERLKSREFIKGDDIYILLTVEDISHLNSTSAVPDPVPTLAVHNACSEVVCQNGGICVVQDGRAE CKCPAGEDWWYMGKRCEKRGSTRDTVIIAVSSTVTVFAVMLIITLVSVYCTRRKYRKKARANTAAMTLEN QHAF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008586 |
| ORF Size | 2112 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008586.2, NP_032612.2 |
| RefSeq Size | 2286 bp |
| RefSeq ORF | 2115 bp |
| Locus ID | 17288 |
| UniProt ID | Q61847 |
| MW | 80 kDa |
| Gene Summary | Membrane metallopeptidase that sheds many membrane-bound proteins. Exhibits a strong preference for acidic amino acids at the P1' position (PubMed:11278902). Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220737 | Mep1b (untagged) - Mouse meprin 1 beta (Mep1b), (10ug) |
CNY 7,744.00 |
|
| MG226519 | Mep1b (tGFP-tagged) - Mouse meprin 1 beta (Mep1b), (10ug) |
CNY 7,700.00 |
|
| MR226519L3 | Lenti ORF clone of Mep1b (Myc-DDK-tagged) - Mouse meprin 1 beta (Mep1b) |
CNY 8,930.00 |
|
| MR226519L4 | Lenti ORF clone of Mep1b (mGFP-tagged) - Mouse meprin 1 beta (Mep1b) |
CNY 8,930.00 |
