Dhx15 (NM_007839) Mouse Tagged ORF Clone
CAT#: MR226475
- TrueORF®
Dhx15 (Myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 15 (Dhx15), transcript variant 2
ORF Plasmid: tGFP
"NM_007839" in other vectors (4)
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CNY 8,736.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | DBP1; Ddx15; DEAH9; HRH2; mDEAH9 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226475 representing NM_007839
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAAGAGGCATCGGTTGGACCTGGGGGAGGATTACCCCTCCGGCAAGAAGCGTGCGGGGACCGATG GGAAAGACCGGGAACGAGATCGGGATAGAGAGGACCGATCTAAAGATCGTGACCGAGAACGTGATAGAGG AGACAGAGAACGGGAGAGGGAAAAAGAAAAGGAAAAAGAATTGAGAGCTTCCACCAATGCTATGCTTATC AGTGCTGGATTGCCACCTTTAAAAGCTTCTCATTCAGCTCACTCAACCCACTCGGCTCATTCAACACATT CTACACATTCTGCCCACTCAACACACACTGGACACACAGGACACACATCACTTCCACAGTGCATTAATCC ATTCACCAACCTGCCCCATACTCCTCGATACTATGATATTCTAAAGAAACGACTTCAGCTCCCTGTATGG GAATACAAGGATAGATTTACAGATATTCTTGTTAGACATCAGTCATTTGTACTTGTTGGTGAGACTGGGT CTGGTAAAACAACACAGATACCACAGTGGTGTGTGGAGTATATGCGATCCTTGCCAGGACCCAAAAGAGG AGTCGCCTGTACACAGCCCAGGAGAGTGGCTGCAATGAGTGTGGCTCAGAGAGTTGCTGATGAGATGGAC GTGATGTTAGGCCAGGAAGTTGGATACTCCATTCGATTTGAAGACTGCAGTAGTGCAAAAACCATTCTTA AGTATATGACTGACGGGATGCTACTTCGTGAAGCCATGAACGATCCCCTTCTGGAGCGTTATGGTGTGAT AATTCTTGATGAGGCCCATGAAAGAACATTGGCTACAGATATTCTCATGGGTGTTCTAAAGGAAGTTGTA AGACAGAGATCAGATTTAAAGGTTATAGTTATGAGTGCTACCCTAGATGCTGGGAAATTCCAGATTTACT TTGATAACTGTCCTCTCTTAACTATTCCTGGCCGAACACATCCTGTTGAGATTTTTTATACTCCAGAACC AGAGAGAGATTATCTTGAAGCAGCAATTCGGACAGTGATCCAAATACATATGTGTGAAGAGGAGGAAGGA GACCTTCTTCTTTTCTTGACTGGTCAAGAGGAAATTGATGAGGCCTGTAAGAGAATAAAGCGTGAAGTTG ATGATTTGGGCCCTGAAGTTGGTGATATTAAAATCATTCCATTATATTCTACACTTCCACCCCAGCAGCA ACAACGCATTTTTGAGCCACCACCTCCAAAAAAACAGAATGGAGCAATTGGAAGAAAGGTGGTGGTGTCA ACTAATATTGCAGAGACCTCTTTGACAATAGACGGTGTGGTGTTTGTAATTGACCCTGGATTTGCAAAAC AAAAGGTATATAATCCTAGAATCAGAGTCGAGTCTCTTCTGGTGACTGCCATTAGTAAAGCTTCAGCTCA GCAAAGGGCGGGTCGAGCTGGACGGACCAGACCTGGGAAATGCTTCAGGCTTTATACAGAGAAAGCTTAT AAAACAGAAATGCAGGACAACACCTATCCTGAGATCCTGCGCTCTAACCTAGGATCGGTTGTGTTGCAGC TGAAGAAGCTTGGTATTGATGACTTGGTACACTTTGATTTTATGGACCCACCAGCTCCTGAAACTCTGAT GAGAGCCCTAGAACTTTTGAATTATTTGGCTGCTTTAAATGATGATGGAGATCTGACTGAATTGGGATCC ATGATGGCAGAGTTCCCTTTAGATCCACAGCTCGCTAAAATGGTTATTGCAAGTTGTGACTACAACTGTT CTAATGAGGTCCTATCTATTACTGCTATGTTGTCAGTCCCACAGTGTTTTGTTCGCCCCACGGAGGCCAA GAAAGCTGCAGATGAGGCCAAGATGAGATTTGCCCACATAGATGGAGATCATCTGACACTGCTGAATGTC TACCACGCTTTTAAACAAAATCATGAATCTGTTCAGTGGTGTTATGACAACTTCATTAACTACCGGTCCC TGATGTCTGCAGACAATGTACGCCAGCAGCTATCAAGAATTATGGACAGATTTAATTTGCCTCGTCGAAG TACTGATTTCACAAGCAGGGACTATTATATTAATATAAGAAAAGCTTTGGTTACTGGGTATTTTATGCAA GTGGCACATTTAGAACGAACGGGGCATTATTTAACTGTGAAAGATAACCAGGTGGTCCAGTTGCATCCCT CTACTGTGCTTGATCACAAGCCTGAATGGGTGCTTTATAATGAGTTTGTGCTGACGACAAAGAATTATAT CCGGACATGTACAGATATCAAGCCAGAATGGTTGGTGAAAATTGCCCCTCAATATTATGACATGAGCAAT TTCCCACAGTGTGAAGCAAAGAGACAGTTGGACCGCATTATTGCCAAACTTCAATCCAAGGAATATTCAC AGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226475 representing NM_007839
Red=Cloning site Green=Tags(s) MSKRHRLDLGEDYPSGKKRAGTDGKDRERDRDREDRSKDRDRERDRGDREREREKEKEKELRASTNAMLI SAGLPPLKASHSAHSTHSAHSTHSTHSAHSTHTGHTGHTSLPQCINPFTNLPHTPRYYDILKKRLQLPVW EYKDRFTDILVRHQSFVLVGETGSGKTTQIPQWCVEYMRSLPGPKRGVACTQPRRVAAMSVAQRVADEMD VMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVV RQRSDLKVIVMSATLDAGKFQIYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEEEG DLLLFLTGQEEIDEACKRIKREVDDLGPEVGDIKIIPLYSTLPPQQQQRIFEPPPPKKQNGAIGRKVVVS TNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAY KTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGS MMAEFPLDPQLAKMVIASCDYNCSNEVLSITAMLSVPQCFVRPTEAKKAADEAKMRFAHIDGDHLTLLNV YHAFKQNHESVQWCYDNFINYRSLMSADNVRQQLSRIMDRFNLPRRSTDFTSRDYYINIRKALVTGYFMQ VAHLERTGHYLTVKDNQVVQLHPSTVLDHKPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAPQYYDMSN FPQCEAKRQLDRIIAKLQSKEYSQY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007839 |
ORF Size | 2385 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007839.3, NP_031865.2 |
RefSeq Size | 3009 bp |
RefSeq ORF | 2388 bp |
Locus ID | 13204 |
UniProt ID | O35286 |
MW | 91.5 kDa |
Gene Summary | Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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DHX15 Senses Double-Stranded RNA in Myeloid Dendritic Cells
,Lu, H;Lu, N;Weng, L;Yuan, B;Liu, YJ;Zhang, Z;,
J. Immunol.
,PubMed ID 24990078
[Dhx15]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC221687 | Dhx15 (untagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 15 (Dhx15), transcript variant 2, (10ug) |
CNY 10,260.00 |
|
MG226475 | Dhx15 (tGFP-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 15 (Dhx15) transcript variant 2, (10ug) |
CNY 8,650.00 |
|
MR226475L3 | Lenti ORF clone of Dhx15 (Myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 15 (Dhx15), transcript variant 2 |
CNY 9,790.00 |
|
MR226475L4 | Lenti ORF clone of Dhx15 (mGFP-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 15 (Dhx15), transcript variant 2 |
CNY 9,790.00 |