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Prkg1 (NM_001013833) Mouse Tagged ORF Clone
CAT＃: MR226330
- TrueORF®
Prkg1 (Myc-DDK-tagged) - Mouse protein kinase, cGMP-dependent, type I (Prkg1), transcript variant 1
ORF Plasmid: tGFP
"NM_001013833" in other vectors (4)
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CNY 6,650.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW125416; CGKI; Gm19690; Prkg1b; Prkgr1b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226330 representing NM_001013833
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGAACTGGAGGAAGACTTTGCGAAGATTCTCATGCTCAAGGAGGAGAGGATCAAAGAGCTGGAGA AGCGGCTGTCAGAGAAGGAGGAAGAAATCCAGGAGCTGAAGAGGAAACTCCACAAATGCCAGTCGGTGCT GCCGGTGCCCTCCACCCACATCGGCCCGCGGACCACCCGGGCGCAGGGCATCTCCGCGGAGCCGCAGACC TACAGGTCCTTCCACGACCTGCGCCAGGCGTTCCGGAAGTTCACTAAATCCGAGAGGTCGAAGGATCTCA TAAAGGAGGCTATCCTTGACAATGACTTCATGAAGAACTTGGAGCTGTCACAGATCCAGGAGATTGTGGA CTGTATGTACCCCGTGGAATACGGCAAGGACAGCTGCATCATCAAGGAAGGCGATGTGGGGTCACTGGTG TACGTCATGGAAGATGGGAAGGTTGAAGTCACAAAAGAAGGCGTGAAGCTCTGCACCATGGGTCCTGGAA AAGTGTTCGGGGAGCTGGCTATACTTTACAACTGTACCCGGACAGCGACCGTCAAGACTCTTGTAAATGT GAAACTCTGGGCCATCGATCGACAATGTTTTCAAACAATAATGATGAGGACAGGACTCATCAAGCATACC GAGTACATGGAATTTTTAAAAAGTGTTCCAACATTCCAGAGCCTTCCTGATGAAATCCTCAGCAAGCTGG CTGATGTCCTCGAAGAGACCCACTATGAAAATGGAGAATATATCATCAGGCAGGGTGCAAGAGGAGACAC CTTCTTCATCATCAGTAAAGGGCAGGTGAATGTTACTCGAGAAGACTCACCAAGTGAAGACCCAGTCTTC CTTAGAACTTTAGGGAAGGGAGATTGGTTTGGAGAGAAAGCGTTGCAGGGGGAGGATGTGAGAACAGCAA ATGTTATTGCCGCAGAAGCTGTCACCTGCCTTGTGATTGACAGAGACTCTTTCAAGCATTTGATTGGAGG ACTGGACGATGTTTCTAACAAAGCATATGAGGACGCAGAAGCAAAAGCAAAATATGAAGCCGAAGCTGCC TTCTTCGCCAACCTGAAGCTGTCTGATTTCAACATCATTGACACCCTTGGAGTTGGAGGTTTCGGACGAG TAGAGCTGGTCCAGTTGAAAAGTGAAGAATCCAAAACATTTGCAATGAAGATCCTCAAGAAACGCCACAT TGTGGACACCAGACAGCAGGAGCACATCCGCTCAGAGAAGCAGATCATGCAGGGGGCTCATTCTGACTTC ATTGTGAGGCTGTACAGGACATTTAAAGACAGCAAATACTTGTATATGTTGATGGAAGCGTGCCTGGGTG GAGAGCTCTGGACTATTCTCAGGGATAGGGGTTCGTTTGAAGACTCAACAACCAGGTTTTACACTGCATG TGTGGTAGAAGCATTCGCCTATCTGCATTCCAAAGGAATCATTTACAGGGACCTCAAGCCGGAGAATCTC ATCCTAGATCATCGAGGCTATGCCAAACTGGTTGACTTTGGCTTTGCAAAGAAAATAGGATTTGGAAAGA AAACATGGACTTTTTGTGGGACTCCAGAATATGTAGCCCCAGAGATCATCCTGAACAAAGGCCATGACAT TTCAGCTGACTACTGGTCACTAGGAATTCTGATGTATGAGCTTCTGACTGGCAGCCCACCTTTCTCAGGC CCAGATCCAATGAAAACCTACAATATCATACTGCGGGGGATTGACATGATAGAATTTCCAAAGAAGATTG CAAAAAATGCTGCTAATTTAATTAAAAAACTATGCAGGGACAACCCATCAGAGAGGTTAGGAAATTTGAA AAACGGAGTCAAAGACATTCAGAAACACAAGTGGTTTGAGGGCTTTAATTGGGAAGGCTTAAGAAAAGGC ACCTTGACACCTCCCATAATTCCAAGTGTTGCGTCACCCACAGACACAAGCAATTTTGACAGTTTCCCTG AGGACAGCGATGAGCCACCACCTGATGACAACTCAGGCTGGGACATAGACTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226330 representing NM_001013833
Red=Cloning site Green=Tags(s) MSELEEDFAKILMLKEERIKELEKRLSEKEEEIQELKRKLHKCQSVLPVPSTHIGPRTTRAQGISAEPQT YRSFHDLRQAFRKFTKSERSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLV YVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHT EYMEFLKSVPTFQSLPDEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGQVNVTREDSPSEDPVF LRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAA FFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDF IVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENL ILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSG PDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKG TLTPPIIPSVASPTDTSNFDSFPEDSDEPPPDDNSGWDIDF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001013833 |
| ORF Size | 2013 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001013833.3, NP_001013855.1 |
| RefSeq Size | 3914 bp |
| RefSeq ORF | 2016 bp |
| Locus ID | 19091 |
| UniProt ID | P0C605 |
| MW | 76.8 kDa |
| Gene Summary | Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways: phosphorylates MRVI1/IRAG and inhibits IP3-induced Ca(2+) release from intracellular stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellular Ca(2+) levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regulation of RHOA translocation; decreasing contraction; controlling vesicle trafficking, reduction of myosin light chain phosphorylation resulting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regulation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. Regulates vasodilator-stimulated phosphoprotein (VASP) functions in platelets and smooth muscle.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220322 | Prkg1 (untagged) - Mouse protein kinase, cGMP-dependent, type I (Prkg1), transcript variant 1, (10ug) |
CNY 4,920.00 |
|
| MG226330 | Prkg1 (tGFP-tagged) - Mouse protein kinase cGMP-dependent type I (Prkg1) transcript variant 1, (10ug) |
CNY 7,008.00 |
|
| MR226330L3 | Lenti ORF clone of Prkg1 (Myc-DDK-tagged) - Mouse protein kinase, cGMP-dependent, type I (Prkg1), transcript variant 1 |
CNY 8,550.00 |
|
| MR226330L4 | Lenti ORF clone of Prkg1 (mGFP-tagged) - Mouse protein kinase, cGMP-dependent, type I (Prkg1), transcript variant 1 |
CNY 8,550.00 |
