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Krit1 (NM_030675) Mouse Tagged ORF Clone
CAT＃: MR226187
- TrueORF®
Krit1 (Myc-DDK-tagged) - Mouse KRIT1, ankyrin repeat containing (Krit1), transcript variant 1
ORF Plasmid: tGFP
"NM_030675" in other vectors (4)
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CNY 5,392.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2010007K12Rik; A630036P20Rik; AA432855; AI450393; AI643869; BB155247; BB235701; Ccm1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226187 representing NM_030675
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAATCCAGAAAACATCGAAGATGCTTACGTTGCAGTTATTCGTCCAAAGAACACTGCTAGTCTCA ACTCCCGGGAGTATAGAGCTAAGTCCTATGAAATTTTATTGCATGAAGTTCCCATTGAAGGACAGAAAAA AAAGCGAAAGAAAGTTTTGCTGGAAACTAAACTTCAAAGCAACAGTGAAATAGCACAAGGCATATTGGAC TATGTAGTAGAAACTACCAAACCAATTTCTCCTGCAAACCAGGGGATTAAAGGGAAACGAGTGGTGCTGA TGAGGAAGTTTCCTCTGGACGGAGAGAAGACAGGCAGAGAAGCAGCACTGTTTATCGTGCCATCAGTTGT CAAAGATAATACTAAATATGCATATACTCCTGGATGCCCAATTTTTTACTGCTTACAAGATATTATGAGA GTTTGTAGTGAATCCAGTACTCACTTTGCAACACTTACAGCAAGGATGTTAATAGCCTTGGATAAGTGGT TAGATGAACGTCATGCGCAGTCTCACTTTATTCCAGCTTTATTCCGACCTTCTCCCCTTGAACGGATAAA GACAAATGTCATAAACCCTGCGTATGCTGCTGAATTAGGCCAGGTAGACAATTCACTACATATGGGCTAT AGTGCACTAGAAATAAAGAGTAAAATGCTAGCCCTAGAGAAAGCAGACACCTGCATTTACAACCCTTTGT TTGGATCAGATCTTCAGTATACAAATCGGGTAGATAAAGTGGTAATAAATCCATACTTTGGTCTCGGAGC TCCAGACTACTCAAAAATCCAAATTCCCAAACAGGAAAAATGGCAGCGAAGCATGAGCAGCGTTGTGGAA GACAAAGAACGACAGTGGGTTGATGACTTTCCTTTACATCGAAATGCCTGTGAAGGAGATTCAGAATTAC TGAGCCATCTTCTCGATAAAGGACTTTCAGTCAACCAACTAGATAATGACCACTGGGCACCCATTCATTA TGCATGCTGGTATGGAAAAGTTGAGGCCACTCGCATATTATTAGAGAAAGGAAAGTGCAATCCAAACCTT TTAAATGGGCAGCTCAGCTCACCGCTTCACTTTGCTGCTGGAGGCGGCCATGCTGAAATAGTGCAGATCC TCCTGACTCACCCAGACATTGACAGGCACATAACAGATCAACAAGGAAGATCCCCATTAAATGTTTGTGA AGAAAACAAACAAAATAACTGGGAAGAAGCTGCAAAATTGTTGAAAGACGCCATTAACAAGCCATATGAA AAAGTTCGAATCTATAGAATGGATGGATCATACCGTTCTGTTGAACTAAAGCATGGCAATAATACCACAG CACAGCAGATAATGGAGGGAATGCGGCTCTCTCAGGAAACTCAGCGATATTTCACTATTTGGATCTGTTC AGAAAATCTTAGTCTTCAGTTCAAGCCTTATCATAAACCCTTGCAACAAGTTCATGACTGGCCAGAAATA CTTGCTGAATTGACTAATTTGGATCCACAAAGAGAAACACCACAGCTTTTCCTAAGAAGAGATGTGGGAC TTCCTTTAGAAGTTGAGAAAAAGATTGAAGACCCACTAGCTATTCTTATTCTCTTTGATGAAGCCAGATA TAATTTACTGAAGGGCTTTTATACAGCTCCTGATGCTAAACTGATAACACTGGCAAGTCTACTGTTACAA ATAGTTTATGGGAATTATGAGAGTAAAAAGCACAAACAAGGTTTCTTAAATGAAGAAACTCTGAAATCCA TCGTACCTATTACTAAACTGAAAAGTAAGGCGCCTCACTGGATAAACCGAATACTCCATGAGTACAAGAA TCTGAGTCTGAGTGAGGGCGTCAGTAAGGAAATGCACCACCTGCAGCGCATGTTCCTACAGAACTGCTGG GAGATCCCTACGTACGGAGCCGCCTTCTTCACAGGACAGATATTTACAAAGGCAAGCCCAAGCAATCATA AAGTCATCCCTGTGTATGTAGGAGTGAATATAAAAGGACTTCATCTCCTGAACATGGAAACTAAGGCATT ACTCATCAGTCTCAAGTACTGTTGCTTTACGTGGCAGCTGGGAGATGCTGGTACTTGTTTTCAAATCCAT AGTATGGAAAATAAAATGAGCTTTATAGTACACACAAAACAGGCTGGCCTTGTGGTAAAGCTGCTAATGA AGTTAAATGGACAACTAATGCCCTCTGAAAGAAATTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226187 representing NM_030675
Red=Cloning site Green=Tags(s) MGNPENIEDAYVAVIRPKNTASLNSREYRAKSYEILLHEVPIEGQKKKRKKVLLETKLQSNSEIAQGILD YVVETTKPISPANQGIKGKRVVLMRKFPLDGEKTGREAALFIVPSVVKDNTKYAYTPGCPIFYCLQDIMR VCSESSTHFATLTARMLIALDKWLDERHAQSHFIPALFRPSPLERIKTNVINPAYAAELGQVDNSLHMGY SALEIKSKMLALEKADTCIYNPLFGSDLQYTNRVDKVVINPYFGLGAPDYSKIQIPKQEKWQRSMSSVVE DKERQWVDDFPLHRNACEGDSELLSHLLDKGLSVNQLDNDHWAPIHYACWYGKVEATRILLEKGKCNPNL LNGQLSSPLHFAAGGGHAEIVQILLTHPDIDRHITDQQGRSPLNVCEENKQNNWEEAAKLLKDAINKPYE KVRIYRMDGSYRSVELKHGNNTTAQQIMEGMRLSQETQRYFTIWICSENLSLQFKPYHKPLQQVHDWPEI LAELTNLDPQRETPQLFLRRDVGLPLEVEKKIEDPLAILILFDEARYNLLKGFYTAPDAKLITLASLLLQ IVYGNYESKKHKQGFLNEETLKSIVPITKLKSKAPHWINRILHEYKNLSLSEGVSKEMHHLQRMFLQNCW EIPTYGAAFFTGQIFTKASPSNHKVIPVYVGVNIKGLHLLNMETKALLISLKYCCFTWQLGDAGTCFQIH SMENKMSFIVHTKQAGLVVKLLMKLNGQLMPSERNS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030675 |
| ORF Size | 2208 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030675.3, NP_109600.2 |
| RefSeq Size | 6105 bp |
| RefSeq ORF | 2211 bp |
| Locus ID | 79264 |
| UniProt ID | Q6S5J6 |
| MW | 84 kDa |
| Gene Summary | Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity. Negative regulator of angiogenesis. Inhibits endothelial proliferation, apoptosis, migration, lumen formation and sprouting angiogenesis in primary endothelial cells. Promotes AKT phosphorylation in a NOTCH-dependent and independent manner, and inhibits ERK1/2 phosphorylation indirectly through activation of the DELTA-NOTCH cascade. Acts in concert with CDH5 to establish and maintain correct endothelial cell polarity and vascular lumen and these effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction, and cell junction stabilization. Plays a role in integrin signaling via its interaction with ITGB1BP1; this prevents the interaction between ITGB1 and ITGB1BP1. Microtubule-associated protein that binds to phosphatidylinositol 4,5-bisphosphate (PIP2)-containing membranes in a GTP-bound RAP1-dependent manner (By similarity). Plays an important role in the maintenance of the intracellular reactive oxygen species (ROS) homeostasis to prevent oxidative cellular damage. Regulates the homeostasis of intracellular ROS through an antioxidant pathway involving FOXO1 and SOD2. Facilitates the down-regulation of cyclin-D1 (CCND1) levels required for cell transition from proliferative growth to quiescence by preventing the accumulation of intracellular ROS through the modulation of FOXO1 and SOD2 levels.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221186 | Krit1 (untagged) - Mouse KRIT1, ankyrin repeat containing (Krit1), transcript variant 1, (10ug) |
CNY 9,500.00 |
|
| MG226187 | Krit1 (tGFP-tagged) - Mouse KRIT1 ankyrin repeat containing (Krit1) transcript variant 1, (10ug) |
CNY 6,370.00 |
|
| MR226187L3 | Lenti ORF clone of Krit1 (Myc-DDK-tagged) - Mouse KRIT1, ankyrin repeat containing (Krit1), transcript variant 1 |
CNY 7,700.00 |
|
| MR226187L4 | Lenti ORF clone of Krit1 (mGFP-tagged) - Mouse KRIT1, ankyrin repeat containing (Krit1), transcript variant 1 |
CNY 7,700.00 |
